Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	596,266	C→A	N153K (AAC→AAA)	U712_02945 →	Mannan endo‑1,4‑beta‑mannosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	596,266	0	C	A	100.0%	307.5 / NA	96	N153K (AAC→AAA)	U712_02945	Mannan endo‑1,4‑beta‑mannosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/58);  total (38/58)

GATGTAAGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAACATATTAGATTCAGCAACAGCGGAAGGGAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTGCATGAAATGAACGGCGAATGG  >  CP006881/596132‑596401                                                                                                                                       |                                                                                                                                        gATGTAAGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAatac                                                                                                                                     >  1:40980/1‑138 (MQ=255)   tGTAAGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAatac                                                                                                                                     >  7:148557/1‑136 (MQ=255)   tGTAAGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTa                                                                                                                                   <  7:368908/139‑1 (MQ=255)   tGTAAGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTa                                                                                                                                   <  5:210535/139‑1 (MQ=255)    gTAAGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAatac                                                                                                                                     >  2:118022/1‑135 (MQ=255)    gTAAGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAatac                                                                                                                                     >  7:89729/1‑135 (MQ=255)     tAAGCTGCAACGGCGATTTAATGTCGTATTGGAAATATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGa                                                                                                                                 <  3:426594/139‑1 (MQ=255)     tAAGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAatac                                                                                                                                     >  6:186562/1‑134 (MQ=255)     tAAGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGa                                                                                                                                 <  4:348059/139‑1 (MQ=255)       aGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGAtt                                                                                                                               >  7:72026/1‑139 (MQ=255)       aGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGAtt                                                                                                                               >  3:180479/1‑139 (MQ=255)       aGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGATTTTCAGTCAGGGCATTTTAAAACACCGATTACAAAAGATCAGTATAAAAAAATACTAGAt                                                                                                                                >  1:409144/1‑138 (MQ=255)       aGCTGCAACGGCGATTCAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGTCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGAt                                                                                                                                >  8:329176/1‑138 (MQ=255)        gCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTc                                                                                                                              <  2:347701/139‑1 (MQ=255)            cAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTcttc                                                                                                                           >  6:261265/1‑135 (MQ=255)            cAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTctta                                                                                                                           >  4:396368/1‑135 (MQ=255)            cAACGGCGATTTAAAGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTcttc                                                                                                                           <  2:130535/138‑4 (MQ=255)             aaCGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTcttcaa                                                                                                                         <  1:404602/139‑6 (MQ=255)             aaCGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTcttcaa                                                                                                                         <  4:146078/139‑6 (MQ=255)             aaCGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTcttcaa                                                                                                                         <  8:200124/139‑6 (MQ=255)             aaCGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTcttcaa                                                                                                                         >  5:140675/1‑134 (MQ=255)               cGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTcttcaa                                                                                                                         >  6:235919/1‑132 (MQ=255)                ggCGATTTAATGTCGTATTGGAAAAATGTAGGAATTCCGCAAATCAGTTTGCACCTGTCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAg                                                                                                                      <  8:154924/139‑1 (MQ=255)                ggCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAg                                                                                                                      <  8:72026/139‑1 (MQ=255)                    aTTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAgtag                                                                                                                   >  5:319453/1‑135 (MQ=255)                            tCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTagaaggaaag                                                                                                           <  3:380251/138‑1 (MQ=255)                              gTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATCCTAGATTCTTCAACAGTAGAAGGAAAGCgg                                                                                                        <  5:128903/139‑1 (MQ=255)                               tATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGc                                                                                                       >  5:254923/1‑139 (MQ=255)                               tATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGc                                                                                                       >  3:435242/1‑139 (MQ=255)                                 tttgAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCATGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCt                                                                                                      <  2:283936/135‑1 (MQ=255)                                 ttGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCt                                                                                                      <  7:260785/138‑1 (MQ=255)                                 ttGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCt                                                                                                      <  6:319453/138‑1 (MQ=255)                                 ttGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCt                                                                                                      <  4:12572/138‑1 (MQ=255)                                 ttGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCt                                                                                                      <  1:246223/138‑1 (MQ=255)                                 ttGGAAAAATGGCGGAAATCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCt                                                                                                      <  4:399801/138‑1 (MQ=255)                                    gAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAAt                                                                                                  >  6:238673/1‑139 (MQ=255)                                      aaaaTGGCGGAATTACGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATAATAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGc                                                                                                >  2:102924/1‑139 (MQ=255)                                       aaaTGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTTCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTATATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGcc                                                                                               <  7:329176/139‑1 (MQ=255)                                       aaaTGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGcc                                                                                               <  1:428697/139‑1 (MQ=255)                                       aaaTGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGcc                                                                                               <  1:140776/139‑1 (MQ=255)                                         aTGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCAt                                                                                             >  8:420035/1‑139 (MQ=255)                                          tGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATg                                                                                            <  7:420035/139‑1 (MQ=255)                                           ggCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCATCAGTAGAAGGAAAGCGGATAAATGCCATGc                                                                                           >  5:9187/1‑139 (MQ=255)                                                 aTTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTATCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCa                                                                                     <  5:423517/139‑1 (MQ=255)                                                  atCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATACTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCa                                                                                     >  1:222473/2‑138 (MQ=255)                                                           tCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGa                                                                           <  7:282603/139‑1 (MQ=255)                                                             aGTTTTCACCTGGCGAACACTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATTAAAAAATACTAGATTCTTCAACTGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACg                                                                         <  2:409144/139‑1 (MQ=255)                                                             aGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACg                                                                         <  4:360989/139‑1 (MQ=255)                                                                        ggCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAagag                                                              >  1:206664/1‑139 (MQ=255)                                                                         gCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCg                                                                                                         >  1:101488/1‑95 (MQ=255)                                                                         gCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCg                                                                                                         <  2:101488/95‑1 (MQ=255)                                                                         gCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATg                                                                                                 >  3:71154/1‑103 (MQ=255)                                                                         gCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATg                                                                                                 <  4:71154/103‑1 (MQ=255)                                                                         gCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGt                                                             >  7:410700/1‑139 (MQ=255)                                                                         gCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGt                                                             >  5:2488/1‑139 (MQ=255)                                                                           gAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTg                                                           <  5:186562/139‑1 (MQ=255)                                                                             aCCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGga                                                         <  2:206664/139‑1 (MQ=255)                                                                             aCCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGga                                                         <  8:89729/139‑1 (MQ=255)                                                                                       tcagtcagGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAgg                                                <  3:251318/138‑1 (MQ=255)                                                                                       tcagtcagGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAgg                                                <  7:400688/138‑1 (MQ=255)                                                                                       tcagtcagGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAgg                                                <  4:435242/138‑1 (MQ=255)                                                                                           tcagGGCATTTTAAAACACCTATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGc                                           <  3:99873/139‑1 (MQ=255)                                                                                           tcagGGCATTTTAAAACACCTATTACAAATGATAATTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGc                                           <  7:9033/139‑1 (MQ=255)                                                                                           tcagGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGc                                           >  7:80448/1‑139 (MQ=255)                                                                                           tcagGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGc                                           <  2:428846/139‑1 (MQ=255)                                                                                           tcagGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGc                                           <  2:40980/139‑1 (MQ=255)                                                                                           tcagGGAATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTAAACAGTAGAAGGAAAGCTGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGc                                           <  4:308074/139‑1 (MQ=255)                                                                                            cagGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGc                                           <  8:80448/138‑1 (MQ=255)                                                                                                gCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCctgtt                                      <  6:172429/139‑1 (MQ=255)                                                                                                gCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCctgtt                                      <  3:219657/139‑1 (MQ=255)                                                                                                gCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCctgtt                                      <  6:279569/139‑1 (MQ=255)                                                                                                gCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCctgtt                                      <  8:76873/139‑1 (MQ=255)                                                                                                gCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCctgtt                                      <  5:27555/139‑1 (MQ=255)                                                                                                gCATTTTAAAACACCGAATACAAATCATCCGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCctgtt                                      <  5:299681/139‑1 (MQ=255)                                                                                                gCACTTTAAAACACCGATTACAAATTATCATTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGATAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCctgtt                                      <  6:180882/139‑1 (MQ=255)                                                                                                   ttttAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCTACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGCCTTCACGAGTTGGAGACCCAAGGTGTGCctgttctg                                   >  8:9033/1‑139 (MQ=255)                                                                                                    tttAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCctgttctgt                                  >  1:288752/1‑139 (MQ=255)                                                                                                     ttAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCctgttctgtt                                 >  5:401955/1‑139 (MQ=255)                                                                                                        aaaCACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCa                               <  1:118022/138‑1 (MQ=255)                                                                                                         aaCACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAgg                             >  5:330752/1‑139 (MQ=255)                                                                                                            acCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCg                          <  7:235999/139‑1 (MQ=255)                                                                                                            acCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCg                          <  1:102924/139‑1 (MQ=255)                                                                                                            acCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCg                          <  5:261265/139‑1 (MQ=255)                                                                                                            acCGATTAAAAATTATCACTATAAAAAAATACTAGAATCTTCAACAGTACAAGGAAAGCCTCTAAATGCCATGCTCAGCAAAATTCCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCg                          <  8:148557/139‑1 (MQ=255)                                                                                                              cGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCt                        >  4:211656/1‑139 (MQ=255)                                                                                                              cGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCt                        >  2:243500/1‑139 (MQ=255)                                                                                                                aTTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTg                       >  1:107151/1‑138 (MQ=255)                                                                                                                aTTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTg                       >  3:236598/1‑138 (MQ=255)                                                                                                                     aaaTGATCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTGCatgaa                 <  1:243500/139‑1 (MQ=255)                                                                                                                         gATCATTATAAAAAAATTCTAGATTCTTCAACAGTAGATGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGAATTCAAGAGTTTGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTGCatgaaatg              <  2:222473/138‑1 (MQ=255)                                                                                                                          aTCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTGCatgaaatgaa            <  7:284483/139‑1 (MQ=255)                                                                                                                          aTCAGTATAAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTGCatgaaatgaa            >  7:193496/1‑139 (MQ=255)                                                                                                                               tataAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTGCATGAAATGAACGGc        >  3:93061/1‑138 (MQ=255)                                                                                                                               tataAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTGCATGAAATGAACGGc        >  5:30545/1‑138 (MQ=255)                                                                                                                                 taAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTGCATGAAATGAACGGCGaa     <  2:288752/139‑1 (MQ=255)                                                                                                                                 taAAAAAATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTGCATGAAATGAACGGCGaa     <  5:235919/139‑1 (MQ=255)                                                                                                                                    aaaaATACTAGATTCTTCAACAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTGCATGAAATGAACGGCGAATgg  >  6:91597/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                        GATGTAAGCTGCAACGGCGATTTAATGTCGTATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGTCAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAACATATTAGATTCAGCAACAGCGGAAGGGAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGTTGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTGCATGAAATGAACGGCGAATGG  >  CP006881/596132‑596401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑