Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,187,583	T→C	K215K (AAA→AAG)	U712_10960 ←	Prephenate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,187,583	0	T	C	100.0%	159.7 / NA	51	K215K (AAA→AAG)	U712_10960	Prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/29);  total (22/29)

CGTCTCAATTTCACGAATCCACTCTTCAAAACGGTCCAAGATTTTATCTTTATTATGTAATAGAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCCCTGAACCCTCCGGCAGCAAAACGTTTAACAAGCGGATAAAGTTCTTCCGAATGATGGGTTTGATGAACTAGGCTTGCTGCGACAATATGCGGAAAATGGCTGATCACGCTTGTAACACCATCATGCTCTTCCGGCGACATTTCCACAAAATGGG  >  CP006881/2187448‑2187713                                                                                                                                        |                                                                                                                                   cttgtcAATTTCACGAATCCACTCATCAAAACGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGctt                                                                                                                                  >  2:67961/5‑138 (MQ=255)    gtcAATTTCACGAATCCACTCATCAAAACGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTacc                                                                                                                               >  2:358432/2‑136 (MQ=255)     tcAATTTCACGAATCCACTCATCAAAACGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACaa                                                                                                                             <  7:413831/139‑1 (MQ=255)      cAATTTCACGAATCCACTCATCAAAACGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAg                                                                                                                            <  1:312066/139‑1 (MQ=255)       aaTTTCACGAATCCACTCATCAAAACGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACaa                                                                                                                             <  8:247408/137‑1 (MQ=255)       aaTTTCACGAATCCACTCATCAAAACGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACaa                                                                                                                             >  7:247408/1‑137 (MQ=255)         tttCACGAATCCACTCATCAAAACGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCg                                                                                                                          >  8:77322/1‑138 (MQ=255)                 aTCCACTCATCAAAACGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAggt                                                                                                                 <  1:126366/139‑3 (MQ=255)                     aCTCATCAAAACGGTTTAAGATTTTATATTTATAATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGgttttc                                                                                                             <  3:40424/139‑1 (MQ=255)                           cAAAACGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAat                                                                                                        >  2:255228/1‑138 (MQ=255)                            aaaaCGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAatg                                                                                                       <  6:144000/138‑1 (MQ=255)                                   tctaaGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGttt                                                                                               >  2:385286/4‑139 (MQ=255)                                        gATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTtggtga                                                                                          <  1:71282/139‑1 (MQ=255)                                         aTTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCGGCTTGATGCACTCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTAAACAAGCGGATACAGGTTTTCCGAATGATGGGTGTGGTGaa                                                                                         <  6:6836/139‑1 (MQ=255)                                          ttttATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAAc                                                                                        <  7:77322/139‑1 (MQ=255)                                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGCTGCAATCCTTGTACTATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCGGAATGAGGGGTTTGGTGAACAAg                                                                                     >  3:26762/1‑139 (MQ=255)                                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTtg                                                                                              <  1:321216/130‑1 (MQ=255)                                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTtg                                                                                              >  2:321216/1‑130 (MQ=255)                                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAAc                                                                                        >  3:260043/1‑136 (MQ=255)                                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAAc                                                                                        <  4:260043/136‑1 (MQ=255)                                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAg                                                                                     >  7:224794/1‑139 (MQ=255)                                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAg                                                                                     >  8:209350/1‑139 (MQ=255)                                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAg                                                                                     >  5:239690/1‑139 (MQ=255)                                               tCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAgg                                                                                    <  4:303839/138‑1 (MQ=255)                                                   tattatGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCtag                                                                                <  3:219752/138‑3 (MQ=255)                                                   tattatGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCtag                                                                                <  8:41020/138‑3 (MQ=255)                                                   tattatGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCtag                                                                                <  6:325203/138‑3 (MQ=255)                                                   tattatGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCt                                                                                  <  4:58536/136‑1 (MQ=255)                                                   tattatGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCt                                                                                  <  1:296322/136‑1 (MQ=255)                                                         gTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATgg                                                                                                   >  5:211369/1‑113 (MQ=255)                                                         gTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATgg                                                                                                   <  6:211369/113‑1 (MQ=255)                                                               aaaTATCCCGCCACATTGCCGGGCTTCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATg                                                                   <  1:437060/138‑1 (MQ=255)                                                                    tCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGa                                                               >  2:126327/1‑138 (MQ=255)                                                                              ttGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                    <  1:255228/139‑1 (MQ=255)                                                                                 ccGGGCTGCTTGATGCAATCATTGTAATATCTCTGTCCCCGCCGGCAGCATAACGCTTAACACGCGGATACAGGTTTTCCGAATGCTGGGGTTGGTGAACAAGGCTAGCTGCTACAATTTGCGGAAAATGACTGATTAc                                                 <  5:252487/139‑1 (MQ=255)                                                                                         ctTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTaa                                         <  4:329125/139‑1 (MQ=255)                                                                                            gATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATTCAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAacg                                       <  3:303320/138‑2 (MQ=255)                                                                                               gCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCcat                                   >  8:56326/1‑139 (MQ=255)                                                                                                   tCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCtag                                                                                <  5:305840/90‑3 (MQ=255)                                                                                                   tCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATg                               <  1:410175/139‑1 (MQ=255)                                                                                                   tCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATg                               <  6:433136/139‑1 (MQ=255)                                                                                                     cTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGct                             <  1:126327/139‑1 (MQ=255)                                                                                                               ctcTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGT‑TTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGa                   >  7:235192/3‑138 (MQ=255)                                                                                                                 cTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACa                 >  6:403720/1‑139 (MQ=255)                                                                                                                      cccGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                    >  7:398322/1‑99 (MQ=255)                                                                                                                      cccGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                    <  8:398322/99‑1 (MQ=255)                                                                                                                         gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac          >  1:141571/2‑138 (MQ=255)                                                                                                                         gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac          >  8:251851/2‑138 (MQ=255)                                                                                                                         gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac          >  6:157069/2‑138 (MQ=255)                                                                                                                         gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac          >  1:285686/2‑138 (MQ=255)                                                                                                                          ccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcaca         >  8:250613/1‑138 (MQ=255)                                                                                                                                gcaAAACGCTTAACAAGCTGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATggg  <  1:177696/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   CGTCTCAATTTCACGAATCCACTCTTCAAAACGGTCCAAGATTTTATCTTTATTATGTAATAGAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCCCTGAACCCTCCGGCAGCAAAACGTTTAACAAGCGGATAAAGTTCTTCCGAATGATGGGTTTGATGAACTAGGCTTGCTGCGACAATATGCGGAAAATGGCTGATCACGCTTGTAACACCATCATGCTCTTCCGGCGACATTTCCACAAAATGGG  >  CP006881/2187448‑2187713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑