Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,187,598	A→C	L210L (CTT→CTG)	U712_10960 ←	Prephenate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,187,598	0	A	C	100.0%	184.2 / NA	58	L210L (CTT→CTG)	U712_10960	Prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/36);  total (22/36)

ATCCACTCTTCAAAACGGTCCAAGATTTTATCTTTATTATGTAATAGAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCCCTGAACCCTCCGGCAGCAAAACGTTTAACAAGCGGATAAAGTTCTTCCGAATGATGGGTTTGATGAACTAGGCTTGCTGCGACAATATGCGGAAAATGGCTGATCACGCTTGTAACACCATCATGCTCTTCCGGCGACATTTCCACAAAATGGGCATTTGTCCCCTTCAGCA  >  CP006881/2187464‑2187731                                                                                                                                       |                                                                                                                                      aTCCACTCATCAAAACGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAggt                                                                                                                                   <  1:126366/139‑3 (MQ=255)     aCTCATCAAAACGGTTTAAGATTTTATATTTATAATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGgttttc                                                                                                                               <  3:40424/139‑1 (MQ=255)           cAAAACGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAat                                                                                                                          >  2:255228/1‑138 (MQ=255)            aaaaCGGTCTAAGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAatg                                                                                                                         <  6:144000/138‑1 (MQ=255)                   tctaaGATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGttt                                                                                                                 >  2:385286/4‑139 (MQ=255)                        gATTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTtggtga                                                                                                            <  1:71282/139‑1 (MQ=255)                         aTTTTATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCGGCTTGATGCACTCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTAAACAAGCGGATACAGGTTTTCCGAATGATGGGTGTGGTGaa                                                                                                           <  6:6836/139‑1 (MQ=255)                          ttttATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAAc                                                                                                          <  7:77322/139‑1 (MQ=255)                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGCTGCAATCCTTGTACTATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCGGAATGAGGGGTTTGGTGAACAAg                                                                                                       >  3:26762/1‑139 (MQ=255)                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTtg                                                                                                                >  2:321216/1‑130 (MQ=255)                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTtg                                                                                                                <  1:321216/130‑1 (MQ=255)                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAAc                                                                                                          <  4:260043/136‑1 (MQ=255)                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAAc                                                                                                          >  3:260043/1‑136 (MQ=255)                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAg                                                                                                       >  7:224794/1‑139 (MQ=255)                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAg                                                                                                       >  5:239690/1‑139 (MQ=255)                             tATCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAg                                                                                                       >  8:209350/1‑139 (MQ=255)                               tCTTTATTATGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAgg                                                                                                      <  4:303839/138‑1 (MQ=255)                                   tattatGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCtag                                                                                                  <  6:325203/138‑3 (MQ=255)                                   tattatGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCtag                                                                                                  <  8:41020/138‑3 (MQ=255)                                   tattatGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCtag                                                                                                  <  3:219752/138‑3 (MQ=255)                                   tattatGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCt                                                                                                    <  1:296322/136‑1 (MQ=255)                                   tattatGTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCt                                                                                                    <  4:58536/136‑1 (MQ=255)                                         gTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATgg                                                                                                                     <  6:211369/113‑1 (MQ=255)                                         gTAATAAAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATgg                                                                                                                     >  5:211369/1‑113 (MQ=255)                                               aaaTATCCCGCCACATTGCCGGGCTTCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATg                                                                                     <  1:437060/138‑1 (MQ=255)                                                    tCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGa                                                                                 >  2:126327/1‑138 (MQ=255)                                                              ttGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                      <  1:255228/139‑1 (MQ=255)                                                                 ccGGGCTGCTTGATGCAATCATTGTAATATCTCTGTCCCCGCCGGCAGCATAACGCTTAACACGCGGATACAGGTTTTCCGAATGCTGGGGTTGGTGAACAAGGCTAGCTGCTACAATTTGCGGAAAATGACTGATTAc                                                                   <  5:252487/139‑1 (MQ=255)                                                                         ctTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTaa                                                           <  4:329125/139‑1 (MQ=255)                                                                            gATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATTCAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAacg                                                         <  3:303320/138‑2 (MQ=255)                                                                               gCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCcat                                                     >  8:56326/1‑139 (MQ=255)                                                                                   tCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCtag                                                                                                  <  5:305840/90‑3 (MQ=255)                                                                                   tCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATg                                                 <  1:410175/139‑1 (MQ=255)                                                                                   tCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATg                                                 <  6:433136/139‑1 (MQ=255)                                                                                     cTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGct                                               <  1:126327/139‑1 (MQ=255)                                                                                               ctcTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGT‑TTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGa                                     >  7:235192/3‑138 (MQ=255)                                                                                                 cTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACa                                   >  6:403720/1‑139 (MQ=255)                                                                                                      cccGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                      <  8:398322/99‑1 (MQ=255)                                                                                                      cccGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                      >  7:398322/1‑99 (MQ=255)                                                                                                         gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac                            >  6:157069/2‑138 (MQ=255)                                                                                                         gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac                            >  1:141571/2‑138 (MQ=255)                                                                                                         gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac                            >  8:251851/2‑138 (MQ=255)                                                                                                         gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac                            >  1:285686/2‑138 (MQ=255)                                                                                                          ccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcaca                           >  8:250613/1‑138 (MQ=255)                                                                                                                gcaAAACGCTTAACAAGCTGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATggg                    <  1:177696/139‑1 (MQ=255)                                                                                                                      cgcttAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGTCTATCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCAttcg              <  7:122554/136‑3 (MQ=255)                                                                                                                      cgcttAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCAttcg              <  7:209350/136‑3 (MQ=255)                                                                                                                        cttAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATCTGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTc            <  1:385286/138‑1 (MQ=255)                                                                                                                        cttAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTc            >  6:120770/2‑139 (MQ=255)                                                                                                                           aaCAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTcccc         >  6:1597/1‑139 (MQ=255)                                                                                                                           aaCAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTccc          <  7:56326/138‑1 (MQ=255)                                                                                                                                 cGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                      >  5:284184/1‑72 (MQ=255)                                                                                                                                 cGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                      <  6:284184/72‑1 (MQ=255)                                                                                                                                  ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca  <  7:39927/139‑1 (MQ=255)                                                                                                                                  ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca  <  8:224794/139‑1 (MQ=255)                                                                                                                                  ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca  <  4:301247/139‑1 (MQ=255)                                                                                                                                  ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca  <  5:1597/139‑1 (MQ=255)                                                                                                                                  ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACACTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca  <  5:392385/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      ATCCACTCTTCAAAACGGTCCAAGATTTTATCTTTATTATGTAATAGAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCCCTGAACCCTCCGGCAGCAAAACGTTTAACAAGCGGATAAAGTTCTTCCGAATGATGGGTTTGATGAACTAGGCTTGCTGCGACAATATGCGGAAAATGGCTGATCACGCTTGTAACACCATCATGCTCTTCCGGCGACATTTCCACAAAATGGGCATTTGTCCCCTTCAGCA  >  CP006881/2187464‑2187731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑