Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,187,640	G→T	V196V (GTC→GTA)	U712_10960 ←	Prephenate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,187,640	0	G	T	100.0%	144.3 / NA	47	V196V (GTC→GTA)	U712_10960	Prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/28);  total (19/28)

GAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCCCTGAACCCTCCGGCAGCAAAACGTTTAACAAGCGGATAAAGTTCTTCCGAATGATGGGTTTGATGAACTAGGCTTGCTGCGACAATATGCGGAAAATGGCTGATCACGCTTGTAACACCATCATGCTCTTCCGGCGACATTTCCACAAAATGGGCATTTGTCCCCTTCAGCAGGTTTTTCAATTGTTCTACCGCTTGTCTGTCCGTTTTCTGGC  >  CP006881/2187510‑2187773                                                                                                                                   |                                                                                                                                      aaaTATCCCGCCACATTGCCGGGCTTCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATg                                                                                                                               <  1:437060/138‑1 (MQ=255)      tCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGa                                                                                                                           >  2:126327/1‑138 (MQ=255)                ttGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                                                                <  1:255228/139‑1 (MQ=255)                   ccGGGCTGCTTGATGCAATCATTGTAATATCTCTGTCCCCGCCGGCAGCATAACGCTTAACACGCGGATACAGGTTTTCCGAATGCTGGGGTTGGTGAACAAGGCTAGCTGCTACAATTTGCGGAAAATGACTGATTAc                                                                                                             <  5:252487/139‑1 (MQ=255)                           ctTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTaa                                                                                                     <  4:329125/139‑1 (MQ=255)                              gATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATTCAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAacg                                                                                                   <  3:303320/138‑2 (MQ=255)                                 gCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCcat                                                                                               >  8:56326/1‑139 (MQ=255)                                     tCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATg                                                                                           <  1:410175/139‑1 (MQ=255)                                     tCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATg                                                                                           <  6:433136/139‑1 (MQ=255)                                       cTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGct                                                                                         <  1:126327/139‑1 (MQ=255)                                                 ctcTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGT‑TTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGa                                                                               >  7:235192/3‑138 (MQ=255)                                                   cTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACa                                                                             >  6:403720/1‑139 (MQ=255)                                                        cccGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                                                                >  7:398322/1‑99 (MQ=255)                                                        cccGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                                                                <  8:398322/99‑1 (MQ=255)                                                           gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac                                                                      >  8:251851/2‑138 (MQ=255)                                                           gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac                                                                      >  6:157069/2‑138 (MQ=255)                                                           gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac                                                                      >  1:141571/2‑138 (MQ=255)                                                           gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac                                                                      >  1:285686/2‑138 (MQ=255)                                                            ccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcaca                                                                     >  8:250613/1‑138 (MQ=255)                                                                  gcaAAACGCTTAACAAGCTGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATggg                                                              <  1:177696/139‑1 (MQ=255)                                                                        cgcttAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGTCTATCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCAttcg                                                        <  7:122554/136‑3 (MQ=255)                                                                        cgcttAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCAttcg                                                        <  7:209350/136‑3 (MQ=255)                                                                          cttAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATCTGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTc                                                      <  1:385286/138‑1 (MQ=255)                                                                          cttAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTc                                                      >  6:120770/2‑139 (MQ=255)                                                                             aaCAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTcccc                                                   >  6:1597/1‑139 (MQ=255)                                                                             aaCAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTccc                                                    <  7:56326/138‑1 (MQ=255)                                                                                   cGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                                                                >  5:284184/1‑72 (MQ=255)                                                                                   cGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                                                                <  6:284184/72‑1 (MQ=255)                                                                                    ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                            <  5:1597/139‑1 (MQ=255)                                                                                    ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                            <  8:224794/139‑1 (MQ=255)                                                                                    ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                            <  7:39927/139‑1 (MQ=255)                                                                                    ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                            <  4:301247/139‑1 (MQ=255)                                                                                    ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACACTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                            <  5:392385/139‑1 (MQ=255)                                                                                               ttcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGTATTCGTCCCCTTCAGCAGGTTTTTTaa                                  <  2:285686/138‑1 (MQ=255)                                                                                               ttcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaac                                 >  4:162875/1‑138 (MQ=255)                                                                                               ttcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                  <  6:187105/138‑1 (MQ=255)                                                                                               ttcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                  <  2:141571/138‑1 (MQ=255)                                                                                               ttcCGAATGATGGGCTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                  <  4:26762/138‑1 (MQ=255)                                                                                                tcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATg                                                                                           >  3:325316/1‑80 (MQ=255)                                                                                                tcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATg                                                                                           <  4:325316/80‑1 (MQ=255)                                                                                                tcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaac                                 <  6:161525/138‑2 (MQ=255)                                                                                                   gAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGATAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCAGTCGTCCCCTTCAGCAGGTTTTTTAACTGtt                             >  6:298915/1‑139 (MQ=255)                                                                                                      tgatgGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTcca                          <  7:250613/139‑3 (MQ=255)                                                                                                            gTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCtt                    >  8:155599/1‑139 (MQ=255)                                                                                                                    aaCAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTcc            <  1:67961/139‑1 (MQ=255)                                                                                                                      caaGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGt          >  8:50425/3‑139 (MQ=255)                                                                                                                         ggCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCAGTCGTCCCCTGCAGCAGGTTTTTTAACTGTTCCCCAGCTTGTGTGTCCGtttt       >  6:35394/1‑139 (MQ=255)                                                                                                                            tagctgctACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTctg    >  8:444560/3‑139 (MQ=255)                                                                                                                              gctgctACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTc                                                      >  6:196368/1‑87 (MQ=255)                                                                                                                              gctgctACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTc                                                      <  5:196368/87‑1 (MQ=255)                                                                                                                              gctgctACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTctggc  >  3:385252/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                      GAATATCCCGCCACATTGCCGGGCTGCTTGATGCAATCCTTGTAATATCCCTGAACCCTCCGGCAGCAAAACGTTTAACAAGCGGATAAAGTTCTTCCGAATGATGGGTTTGATGAACTAGGCTTGCTGCGACAATATGCGGAAAATGGCTGATCACGCTTGTAACACCATCATGCTCTTCCGGCGACATTTCCACAAAATGGGCATTTGTCCCCTTCAGCAGGTTTTTCAATTGTTCTACCGCTTGTCTGTCCGTTTTCTGGC  >  CP006881/2187510‑2187773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑