Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,187,658	G→A	S190S (AGC→AGT)	U712_10960 ←	Prephenate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,187,658	0	G	A	100.0%	240.0 / NA	76	S190S (AGC→AGT)	U712_10960	Prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/39);  total (37/39)

TTGCCGGGCTGCTTGATGCAATCCTTGTAATATCCCTGAACCCTCCGGCAGCAAAACGTTTAACAAGCGGATAAAGTTCTTCCGAATGATGGGTTTGATGAACTAGGCTTGCTGCGACAATATGCGGAAAATGGCTGATCACGCTTGTAACACCATCATGCTCTTCCGGCGACATTTCCACAAAATGGGCATTTGTCCCCTTCAGCAGGTTTTTCAATTGTTCTACCGCTTGTCTGTCCGTTTTCTGGCCTGGCGTTAAAATA  >  CP006881/2187525‑2187787                                                                                                                                      |                                                                                                                                  ttGCCGGGCTGCTTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                                                                              <  1:255228/139‑1 (MQ=255)    ccGGGCTGCTTGATGCAATCATTGTAATATCTCTGTCCCCGCCGGCAGCATAACGCTTAACACGCGGATACAGGTTTTCCGAATGCTGGGGTTGGTGAACAAGGCTAGCTGCTACAATTTGCGGAAAATGACTGATTAc                                                                                                                           <  5:252487/139‑1 (MQ=255)            ctTGATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTaa                                                                                                                   <  4:329125/139‑1 (MQ=255)               gATGCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATTCAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAacg                                                                                                                 <  3:303320/138‑2 (MQ=255)                  gCAATCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCcat                                                                                                             >  8:56326/1‑139 (MQ=255)                      tCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATg                                                                                                         <  1:410175/139‑1 (MQ=255)                      tCCTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATg                                                                                                         <  6:433136/139‑1 (MQ=255)                        cTTGTAATATCTCTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGct                                                                                                       <  1:126327/139‑1 (MQ=255)                                  ctcTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGT‑TTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGa                                                                                             >  7:235192/3‑138 (MQ=255)                                    cTGAACCCGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACa                                                                                           >  6:403720/1‑139 (MQ=255)                                         cccGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                                                                              >  7:398322/1‑99 (MQ=255)                                         cccGCCGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                                                                              <  8:398322/99‑1 (MQ=255)                                            gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac                                                                                    >  1:141571/2‑138 (MQ=255)                                            gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac                                                                                    >  8:251851/2‑138 (MQ=255)                                            gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac                                                                                    >  6:157069/2‑138 (MQ=255)                                            gccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcac                                                                                    >  1:285686/2‑138 (MQ=255)                                             ccGGCAGCAAAACGCTTAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCcaca                                                                                   >  8:250613/1‑138 (MQ=255)                                                   gcaAAACGCTTAACAAGCTGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATggg                                                                            <  1:177696/139‑1 (MQ=255)                                                         cgcttAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGTCTATCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCAttcg                                                                      <  7:122554/136‑3 (MQ=255)                                                         cgcttAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCAttcg                                                                      <  7:209350/136‑3 (MQ=255)                                                           cttAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATCTGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTc                                                                    <  1:385286/138‑1 (MQ=255)                                                           cttAACAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTc                                                                    >  6:120770/2‑139 (MQ=255)                                                              aaCAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTcccc                                                                 >  6:1597/1‑139 (MQ=255)                                                              aaCAAGCGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTccc                                                                  <  7:56326/138‑1 (MQ=255)                                                                    cGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                                                                              <  6:284184/72‑1 (MQ=255)                                                                    cGGATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGAt                                                                                                                              >  5:284184/1‑72 (MQ=255)                                                                     ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                                          <  7:39927/139‑1 (MQ=255)                                                                     ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                                          <  5:1597/139‑1 (MQ=255)                                                                     ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                                          <  8:224794/139‑1 (MQ=255)                                                                     ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                                          <  4:301247/139‑1 (MQ=255)                                                                     ggATACAGGTTTTCCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACACTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                                          <  5:392385/139‑1 (MQ=255)                                                                                ttcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGTATTCGTCCCCTTCAGCAGGTTTTTTaa                                                <  2:285686/138‑1 (MQ=255)                                                                                ttcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaac                                               >  4:162875/1‑138 (MQ=255)                                                                                ttcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                                <  6:187105/138‑1 (MQ=255)                                                                                ttcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                                <  2:141571/138‑1 (MQ=255)                                                                                ttcCGAATGATGGGCTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                                <  4:26762/138‑1 (MQ=255)                                                                                 tcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATg                                                                                                         <  4:325316/80‑1 (MQ=255)                                                                                 tcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATg                                                                                                         >  3:325316/1‑80 (MQ=255)                                                                                 tcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaac                                               <  6:161525/138‑2 (MQ=255)                                                                                    gAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGATAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCAGTCGTCCCCTTCAGCAGGTTTTTTAACTGtt                                           >  6:298915/1‑139 (MQ=255)                                                                                       tgatgGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTcca                                        <  7:250613/139‑3 (MQ=255)                                                                                             gTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCtt                                  >  8:155599/1‑139 (MQ=255)                                                                                                     aaCAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTcc                          <  1:67961/139‑1 (MQ=255)                                                                                                       caaGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGt                        >  8:50425/3‑139 (MQ=255)                                                                                                          ggCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCAGTCGTCCCCTGCAGCAGGTTTTTTAACTGTTCCCCAGCTTGTGTGTCCGtttt                     >  6:35394/1‑139 (MQ=255)                                                                                                             tagctgctACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTctg                  >  8:444560/3‑139 (MQ=255)                                                                                                               gctgctACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTc                                                                    >  6:196368/1‑87 (MQ=255)                                                                                                               gctgctACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTc                                                                    <  5:196368/87‑1 (MQ=255)                                                                                                               gctgctACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTctggc                >  3:385252/1‑139 (MQ=255)                                                                                                                  gctaCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                                          <  7:205113/91‑1 (MQ=255)                                                                                                                  gctaCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                                          >  8:205113/4‑94 (MQ=255)                                                                                                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                                >  6:420967/2‑102 (MQ=255)                                                                                                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                                <  5:420967/101‑1 (MQ=255)                                                                                                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTcc                          >  3:346744/2‑124 (MQ=255)                                                                                                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTcc                          <  4:346744/123‑1 (MQ=255)                                                                                                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTctggcctggc           >  8:188766/2‑139 (MQ=255)                                                                                                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTctggcctggc           >  6:132503/2‑139 (MQ=255)                                                                                                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTctggcctggc           >  1:176303/2‑139 (MQ=255)                                                                                                                       aaTATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGtt        <  7:188766/139‑1 (MQ=255)                                                                                                                        atatGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAACTTGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTa       <  8:34575/139‑1 (MQ=255)                                                                                                                        atatGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTa       <  5:120770/139‑1 (MQ=255)                                                                                                                         tatGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTa       >  5:421112/1‑138 (MQ=255)                                                                                                                         tatGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTGTTCTGGCCTGGCGTTa       >  4:344610/1‑138 (MQ=255)                                                                                                                         tatGCGGAAAATGACTGATTACGATTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGTTTATTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTa       >  5:140665/1‑138 (MQ=255)                                                                                                                           tGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                                          >  2:6421/1‑85 (MQ=255)                                                                                                                           tGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTcagca                                                          <  1:6421/85‑1 (MQ=255)                                                                                                                           tGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGtttttt                                                  >  6:381147/1‑92 (MQ=255)                                                                                                                           tGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGtttttt                                                  <  5:381147/93‑2 (MQ=255)                                                                                                                           tGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTtgtt                               >  4:385747/1‑111 (MQ=255)                                                                                                                           tGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTtgtt                               <  3:385747/112‑2 (MQ=255)                                                                                                                           tGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGtttt                     <  4:234468/122‑1 (MQ=255)                                                                                                                           tGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGtttt                     >  3:234468/1‑122 (MQ=255)                                                                                                                             cGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAata  >  4:178229/1‑139 (MQ=255)                                                                                                                             cGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTGAAAata  >  7:221263/1‑139 (MQ=255)                                                                                                                                aaaaTGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTcca                                        >  7:71461/1‑96 (MQ=255)                                                                                                                                aaaaTGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTcca                                        <  8:71461/98‑3 (MQ=255)                                                                                                                                      |                                                                                                                                  TTGCCGGGCTGCTTGATGCAATCCTTGTAATATCCCTGAACCCTCCGGCAGCAAAACGTTTAACAAGCGGATAAAGTTCTTCCGAATGATGGGTTTGATGAACTAGGCTTGCTGCGACAATATGCGGAAAATGGCTGATCACGCTTGTAACACCATCATGCTCTTCCGGCGACATTTCCACAAAATGGGCATTTGTCCCCTTCAGCAGGTTTTTCAATTGTTCTACCGCTTGTCTGTCCGTTTTCTGGCCTGGCGTTAAAATA  >  CP006881/2187525‑2187787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑