Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,187,739 C→T L163L (TTG→TTA)  U712_10960 ← Prephenate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,187,7390CT98.7% 225.9 / ‑4.6 74L163L (TTG→TTA) U712_10960Prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (42/31);  total (43/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

TTCCGAATGATGGGTTTGATGAACTAGGCTTGCTGCGACAATATGCGGAAAATGGCTGATCACGCTTGTAACACCATCATGCTCTTCCGGCGACATTTCCACAAAATGGGCATTTGTCCCCTTCAGCAGGTTTTTCAATTGTTCTACCGCTTGTCTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAATTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCTGCCATCGGATGCCCCCCGACAAAT  >  CP006881/2187604‑2187871
                                                                                                                                       |                                                                                                                                    
ttcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGTATTCGTCCCCTTCAGCAGGTTTTTTaa                                                                                                                                    <  2:285686/138‑1 (MQ=255)
ttcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaac                                                                                                                                   >  4:162875/1‑138 (MQ=255)
ttcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                                                                                                                    <  2:141571/138‑1 (MQ=255)
ttcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                                                                                                                    <  6:187105/138‑1 (MQ=255)
ttcCGAATGATGGGCTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                                                                                                                    <  4:26762/138‑1 (MQ=255)
 tcCGAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaac                                                                                                                                   <  6:161525/138‑2 (MQ=255)
    gAATGATGGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGATAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCAGTCGTCCCCTTCAGCAGGTTTTTTAACTGtt                                                                                                                               >  6:298915/1‑139 (MQ=255)
       tgatgGGTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTcca                                                                                                                            <  7:250613/139‑3 (MQ=255)
             gTTTGGTGAACAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCtt                                                                                                                      >  8:155599/1‑139 (MQ=255)
                     aaCAAGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTcc                                                                                                              <  1:67961/139‑1 (MQ=255)
                       caaGGCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGt                                                                                                            >  8:50425/3‑139 (MQ=255)
                          ggCTAGCTGCTACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCAGTCGTCCCCTGCAGCAGGTTTTTTAACTGTTCCCCAGCTTGTGTGTCCGtttt                                                                                                         >  6:35394/1‑139 (MQ=255)
                             tagctgctACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTctg                                                                                                      >  8:444560/3‑139 (MQ=255)
                               gctgctACAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTctggc                                                                                                    >  3:385252/1‑139 (MQ=255)
                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                                                                                                                    >  6:420967/2‑102 (MQ=255)
                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTaa                                                                                                                                    <  5:420967/101‑1 (MQ=255)
                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTcc                                                                                                              <  4:346744/123‑1 (MQ=255)
                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTcc                                                                                                              >  3:346744/2‑124 (MQ=255)
                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTctggcctggc                                                                                               >  6:132503/2‑139 (MQ=255)
                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTctggcctggc                                                                                               >  1:176303/2‑139 (MQ=255)
                                    taCAATATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTctggcctggc                                                                                               >  8:188766/2‑139 (MQ=255)
                                       aaTATGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGtt                                                                                            <  7:188766/139‑1 (MQ=255)
                                        atatGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAACTTGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTa                                                                                           <  8:34575/139‑1 (MQ=255)
                                        atatGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTa                                                                                           <  5:120770/139‑1 (MQ=255)
                                         tatGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTa                                                                                           >  5:421112/1‑138 (MQ=255)
                                         tatGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTGTTCTGGCCTGGCGTTa                                                                                           >  4:344610/1‑138 (MQ=255)
                                         tatGCGGAAAATGACTGATTACGATTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGTTTATTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTa                                                                                           >  5:140665/1‑138 (MQ=255)
                                           tGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTtgtt                                                                                                                   <  3:385747/112‑2 (MQ=255)
                                           tGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTtgtt                                                                                                                   >  4:385747/1‑111 (MQ=255)
                                           tGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGtttt                                                                                                         <  4:234468/122‑1 (MQ=255)
                                           tGCGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGtttt                                                                                                         >  3:234468/1‑122 (MQ=255)
                                             cGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAata                                                                                      >  4:178229/1‑139 (MQ=255)
                                             cGGAAAATGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTGAAAata                                                                                      >  7:221263/1‑139 (MQ=255)
                                                aaaaTGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTcca                                                                                                                            >  7:71461/1‑96 (MQ=255)
                                                aaaaTGACTGATTACGCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTcca                                                                                                                            <  8:71461/98‑3 (MQ=255)
                                                              cgcgTGTAACGCCATCAAGCTCCTCTCGCGACATATCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTATAACTGGTCCACAGCTTGTTAGTCCGTGTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCaa                                                                      <  6:92781/138‑1 (MQ=255)
                                                               gCTTGTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAAAGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCaaa                                                                     >  7:402034/1‑138 (MQ=255)
                                                                   gTAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAgg                                                                 <  5:298915/138‑1 (MQ=255)
                                                                    tAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGaa                                                               >  2:433319/1‑139 (MQ=255)
                                                                    tAACGCCATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGaa                                                               >  3:251910/1‑139 (MQ=255)
                                                                         ccATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGaa                                                               >  7:87917/1‑134 (MQ=255)
                                                                         ccATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGaa                                                               <  8:87917/134‑1 (MQ=255)
                                                                         ccATCATGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCt                                                          >  1:229146/1‑139 (MQ=255)
                                                                           atcatGCTCCTCTGGCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTc                                                        >  7:338052/1‑139 (MQ=255)
                                                                            tcatGCTCTTCCGGCGACATTTCCACAAAATGGGCATTTGTCCCCTTCAGCAGGTTTTTCAATTGTTCTACCGCTTGTCTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAATTCCTTCg                                                       >  5:142899/1‑139 (MQ=255)
                                                                                   cctctggCGACATTTCCTCAAAATGGGCATTCGTCCACTTCAGCATGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTTAAATATAAAATGCATTTTCAAACTGGAACTCCTTCGCAGCGGc                                                <  6:239690/134‑1 (MQ=255)
                                                                                    ctctggCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGcc                                               >  3:241956/5‑139 (MQ=255)
                                                                                    ctctggCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGc                                                <  5:132503/134‑1 (MQ=255)
                                                                                    ctctggCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGc                                                <  4:385252/134‑1 (MQ=255)
                                                                                    ctctggCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGc                                                <  7:155599/134‑1 (MQ=255)
                                                                                    ctctggCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGc                                                <  3:162875/134‑1 (MQ=255)
                                                                                    ctctggCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGc                                                <  3:178229/134‑1 (MQ=255)
                                                                                    ctctggCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCA‑TTTCAAACAGGAACTCCTTCGCAGCGGccc                                              >  1:236594/5‑138 (MQ=255)
                                                                                    ctctggCGACATTTCCACAAAATGGGCAATCGTCCCCTTCAGCAGGTTTTTTAACTGATCCACAGCTTGTATGTCCCTTATCTGGCCTGGCGTTATAATATAAAATGCATATTCAAACAGGAACTCCTTCTCATCGccc                                               >  2:205747/5‑139 (MQ=255)
                                                                                      ctggCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAAtata                                                                                    >  1:442016/3‑100 (MQ=255)
                                                                                      ctggCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAAtata                                                                                    <  2:442016/98‑1 (MQ=255)
                                                                                      ctggCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCAc                                             >  3:115761/3‑139 (MQ=255)
                                                                                      ctggCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCACCGGCCAc                                             >  3:37991/3‑139 (MQ=255)
                                                                                         gCGACATTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTc                                           <  4:37991/138‑1 (MQ=255)
                                                                                              aTTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTa                                                                                           >  6:192808/1‑85 (MQ=255)
                                                                                              aTTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTa                                                                                           <  5:192808/85‑1 (MQ=255)
                                                                                              aTTTCCACAAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGAtt                                     >  4:141006/1‑139 (MQ=255)
                                                                                                   cacaAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCa                                                     >  7:437532/1‑118 (MQ=255)
                                                                                                   cacaAAATGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCa                                                     <  8:437532/118‑1 (MQ=255)
                                                                                                        aaTGGGCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAAcc                           >  8:430800/1‑139 (MQ=255)
                                                                                                             gCATTCGTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCa                      >  7:239858/1‑139 (MQ=255)
                                                                                                                ttcgTCCCCTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCg                   >  8:440539/4‑139 (MQ=255)
                                                                                                                        cTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGc                                                <  6:45686/102‑1 (MQ=255)
                                                                                                                        cTTCAGCAGGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGc                                                >  5:45686/1‑102 (MQ=255)
                                                                                                                           cagcagGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTTGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGccctcc         <  8:235192/138‑1 (MQ=255)
                                                                                                                              cagGTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGAc      >  2:107460/1‑138 (MQ=255)
                                                                                                                                 gTTTTTTAACTGTTCCACAGCTTGTTTTTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGc                                                <  5:51108/93‑1 (MQ=255)
                                                                                                                                 gTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGc                                                >  6:51108/1‑93 (MQ=255)
                                                                                                                                 gTTTTTTAACTGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAAt  >  3:48194/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                    
TTCCGAATGATGGGTTTGATGAACTAGGCTTGCTGCGACAATATGCGGAAAATGGCTGATCACGCTTGTAACACCATCATGCTCTTCCGGCGACATTTCCACAAAATGGGCATTTGTCCCCTTCAGCAGGTTTTTCAATTGTTCTACCGCTTGTCTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAATTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCTGCCATCGGATGCCCCCCGACAAAT  >  CP006881/2187604‑2187871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑