Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,187,877	T→G	R117R (CGA→CGC)	U712_10960 ←	Prephenate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,187,877	0	T	G	99.0%	313.2 / ‑4.7	97	R117R (CGA→CGC)	U712_10960	Prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (48/48);  total (48/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

TGTTCTACCGCTTGTCTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAATTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCTGCCATCGGATGCCCCCCGACAAATTGGTATCGGCCAGGCAGCACTTGCTCAGCGTATCGAACCACTTTTTGCTTTGTGCTTCCTACATCGGTAATCAAAAGTTGATGTTCAATTCCTGAATGAGCCAGCTCATCAAGCATAAGCATGGTTTGCGCAACAGGT  >  CP006881/2187743‑2188009                                                                                                                                       |                                                                                                                                     tGTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCTCTCCTGATTTATGTGAACCCGCCATCGGAGGCCCTCCGACAAATTGATAGCGGc                                                                                                                                  <  6:140665/139‑1 (MQ=255)  gTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGc                                                                                                                                  >  7:69353/1‑138 (MQ=255)  gTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGc                                                                                                                                  >  7:126585/1‑138 (MQ=255)  gTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGc                                                                                                                                  >  1:401862/1‑138 (MQ=255)  gTTCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGAATTATGTGAACCCGCCATCGGATGCCCTCCCACAAATTGATAGCgtc                                                                                                                                  >  6:14874/1‑136 (MQ=255)   ttCCACAGCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGc                                                                                                                                  >  5:228584/1‑137 (MQ=255)      cacagCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGgc                                                                                                                             <  3:344610/135‑1 (MQ=255)      cacagCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGgc                                                                                                                             <  7:50425/135‑1 (MQ=255)      cacagCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGgc                                                                                                                             <  5:403720/135‑1 (MQ=255)      cacagCTTGGTTGGCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGgc                                                                                                                             <  7:251851/135‑1 (MQ=255)        cagCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGgcag                                                                                                                           <  8:338052/137‑1 (MQ=255)        cagCTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGgcag                                                                                                                           <  7:440539/137‑1 (MQ=255)           cTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCAc                                                                                                                        >  1:183670/1‑139 (MQ=255)           cTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCAc                                                                                                                        <  6:421112/139‑1 (MQ=255)           cTTGTTTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGAATTATGTGAACCCGCCATCGGATGCCCTCCGACTAATTTATAGCGGCTAGGCAGCAc                                                                                                                        >  4:75572/1‑139 (MQ=255)           cTTGTCTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAATTCCTTCGCAGCGGCCACTCCTGATTTATGTGAAACTGCCATCGGATGCCCCCCGACAAATTGGTATCGGCCAGGCAGCAc                                                                                                                        <  6:142899/139‑1 (MQ=255)             tgtttgtcCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACtt                                                                                                                      >  4:205991/1‑139 (MQ=255)             tgtttgtcCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACtt                                                                                                                      >  2:361385/1‑139 (MQ=255)             tgtttgtcCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACtt                                                                                                                      >  3:195425/1‑139 (MQ=255)                 tgtcCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTgat                                                                                                                   <  5:157069/138‑3 (MQ=255)                 tgtcCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTgat                                                                                                                   <  6:228584/138‑3 (MQ=255)                   tcCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAg                                                                                                                >  6:90449/1‑139 (MQ=255)                     cGTTTTCTGGTCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCg                                                                                                              <  1:70767/139‑1 (MQ=255)                        tttCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTaa                                                                                                           >  7:240969/1‑138 (MQ=255)                              gcctggcGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTaatcaa                                                                                                       <  1:107460/137‑6 (MQ=255)                                  ggcGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGgc                                                                                                                             <  5:215456/111‑1 (MQ=255)                                  ggcGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGgc                                                                                                                             >  6:215456/1‑111 (MQ=255)                                  ggcGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACtt                                                                                                 >  8:297015/1‑139 (MQ=255)                                   gcGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACttt                                                                                                >  1:419858/1‑139 (MQ=255)                                   gcGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACttt                                                                                                <  7:297015/139‑1 (MQ=255)                                   gcGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACttt                                                                                                <  2:183670/139‑1 (MQ=255)                                   gcGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACttt                                                                                                >  7:105590/1‑139 (MQ=255)                                    cGTTAAAATATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACtttt                                                                                               >  3:311792/1‑139 (MQ=255)                                        aaaaTATAAAAAGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCt                                                                                           >  2:265202/1‑139 (MQ=255)                                         aaaTATAAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCt                                                                                           <  4:241956/138‑1 (MQ=255)                                              taAAATGCCTTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTtgtg                                                                                      <  8:221263/138‑1 (MQ=255)                                              taAAATGCATTTTCAAACAGGAACTCCTTCGCAGCGTCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTtgtg                                                                                      <  8:126585/138‑1 (MQ=255)                                                     cATTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTAc                                                                              <  3:205991/139‑1 (MQ=255)                                                      aTTTTCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACa                                                                             >  3:40126/1‑139 (MQ=255)                                                       ttttCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACAt                                                                            <  4:48194/139‑1 (MQ=255)                                                        tttCAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATc                                                                           >  6:11196/1‑139 (MQ=255)                                                           cAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATccg                                                                         <  5:14874/138‑3 (MQ=255)                                                           cAAACAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATccg                                                                         <  8:402034/138‑3 (MQ=255)                                                               cAGGAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATc                                                                    <  4:311792/139‑1 (MQ=255)                                                                  gAACTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGACATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCaa                                                                  >  7:31927/1‑138 (MQ=255)                                                                        cTTCGCAGCGGCCACTCCTGATTTATGTTAACCCGCCATCGGATCCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTGTTTGCTATGTGCTTCCTACTACCGAAATCAAAAgctca                                                           >  4:248454/1‑135 (MQ=255)                                                                        cTTCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAgct                                                             <  1:361385/137‑3 (MQ=255)                                                                          tCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATTCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTg                                                                                                                     <  2:292364/79‑1 (MQ=255)                                                                          tCGCAGCGGCCACTCCTGATTTATGTGAACCCGCCATCGGATTCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTg                                                                                                                     >  1:292364/1‑79 (MQ=255)                                                                                  gCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACACATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTc                                                 <  1:259361/139‑1 (MQ=255)                                                                                  gCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCaaa                                                                 <  7:2686/123‑1 (MQ=255)                                                                                  gCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCaaa                                                                 >  8:2686/1‑123 (MQ=255)                                                                                                ttgtgAACCCGCCATCGGATGCCATCCGACAATTTGATAGCGGCTAGGCAGCACTTGATGAGCGTAATCAACCAGTTTTTGCTTTGTGCTTCCTACATCCGTTATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAg                                    <  1:204922/137‑1 (MQ=255)                                                                                                aTGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAg                                    <  8:239858/138‑1 (MQ=255)                                                                                                aTGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAg                                    <  2:176303/138‑1 (MQ=255)                                                                                                aTGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAg                                    <  4:40126/138‑1 (MQ=255)                                                                                                 tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTc                                                      <  7:177986/119‑1 (MQ=255)                                                                                                 tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTc                                                      >  8:177986/1‑119 (MQ=255)                                                                                                 tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGc                                   >  8:408078/1‑138 (MQ=255)                                                                                                 tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCt                                  >  7:445073/1‑139 (MQ=255)                                                                                                 tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCt                                  >  6:377443/1‑139 (MQ=255)                                                                                                 tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCt                                  >  4:368384/1‑139 (MQ=255)                                                                                                 tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCt                                  >  2:344272/1‑139 (MQ=255)                                                                                                 tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCt                                  >  3:180037/1‑139 (MQ=255)                                                                                                   tgAACCCGCCATCGGATGCCCTCCGACAAATTTATAGCGGCTAGGCTGCACTTGATCAGCGTAATGAAACACTTTTTGCGTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCATATCCTGAATGAGCCAGCtct                                >  5:150840/1‑138 (MQ=255)                                                                                                   tgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTtgtg                                                                                      <  5:78894/85‑1 (MQ=255)                                                                                                   tgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTtgtg                                                                                      >  6:78894/1‑85 (MQ=255)                                                                                                    gAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCtctt                               <  8:445073/139‑3 (MQ=255)                                                                                                     aaCCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCGTGAATGAGCCAg                                    <  6:384754/133‑1 (MQ=255)                                                                                                     aaCCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCtctt                               <  2:236594/138‑3 (MQ=255)                                                                                                     aaCCCGCCATCGGATGCACTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAg                                    >  5:384754/1‑133 (MQ=255)                                                                                                      aCCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAgctc                                                            <  1:332981/108‑4 (MQ=255)                                                                                                      aCCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAgctc                                                            >  2:332981/1‑105 (MQ=255)                                                                                                      aCCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCtcttc                              >  7:68269/1‑138 (MQ=255)                                                                                                      aCCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCtcttc                              >  7:360087/1‑138 (MQ=255)                                                                                                          gCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagca                         <  7:430800/139‑1 (MQ=255)                                                                                                           ccATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagca                         >  6:291034/1‑138 (MQ=255)                                                                                                           ccATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagca                         >  4:340449/1‑138 (MQ=255)                                                                                                           ccATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagaa                         >  2:85703/1‑136 (MQ=255)                                                                                                           ccATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATAAACCACTTTTTGCTTTGTGCTTCCTACAACCGTAATCAAAAGATCCTGTTCAATTCCTGAATGAGCCAGCTCTTCaagca                         >  6:56721/1‑138 (MQ=255)                                                                                                                ggATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataacc                    <  5:90449/138‑3 (MQ=255)                                                                                                                ggATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataacc                    <  8:31927/138‑3 (MQ=255)                                                                                                                  aTGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagt                 <  5:377443/139‑3 (MQ=255)                                                                                                                  aTGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagt                 <  2:229146/139‑3 (MQ=255)                                                                                                                    gCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATACGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagaataaccagtgt               >  4:101259/1‑135 (MQ=255)                                                                                                                     ccctccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCtagcataaccagtgt               <  1:265202/138‑5 (MQ=255)                                                                                                                     ccctccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagtgtt              >  2:166943/1‑134 (MQ=255)                                                                                                                     ccctccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagtgtt              <  8:360087/139‑6 (MQ=255)                                                                                                                     ccctccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagtgt               <  7:408078/138‑5 (MQ=255)                                                                                                                        tccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTgt           >  6:419618/2‑138 (MQ=255)                                                                                                                          cGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACGACTTTTTGCTTTGTGCTTCCTACATACGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTgtt          <  3:75572/138‑3 (MQ=255)                                                                                                                            aCAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTgttcaa       <  5:11196/139‑6 (MQ=255)                                                                                                                            aCAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTgttcaa       >  7:102544/1‑134 (MQ=255)                                                                                                                             cAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAAc      <  4:115761/139‑1 (MQ=255)                                                                                                                                aTTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAgg   >  6:414869/1‑139 (MQ=255)                                                                                                                                aTTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAgg   >  6:198446/1‑139 (MQ=255)                                                                                                                                 ttGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGgt  >  4:224357/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                     TGTTCTACCGCTTGTCTGTCCGTTTTCTGGCCTGGCGTTAAAATATAAAATGCATTTTCAAACAGGAATTCCTTCGCAGCGGCCACTCCTGATTTATGTGAACCTGCCATCGGATGCCCCCCGACAAATTGGTATCGGCCAGGCAGCACTTGCTCAGCGTATCGAACCACTTTTTGCTTTGTGCTTCCTACATCGGTAATCAAAAGTTGATGTTCAATTCCTGAATGAGCCAGCTCATCAAGCATAAGCATGGTTTGCGCAACAGGT  >  CP006881/2187743‑2188009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑