Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,187,949	T→C	Q93E (CAA→GAG)	U712_10960 ←	Prephenate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,187,949	0	T	C	98.9%	289.5 / NA	88	Q93E (CAA→GAG)	U712_10960	Prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (46/41);  minor base A (1/0);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCACTCCTGATTTATGTGAACCTGCCATCGGATGCCCCCCGACAAATTGGTATCGGCCAGGCAGCACTTGCTCAGCGTATCGAACCACTTTTTGCTTTGTGCTTCCTACATCGGTAATCAAAAGTTGATGTTCAATTCCTGAATGAGCCAGCTCATCAAGCATAAGCATGGTTTGCGCAACAGGTGTGGCAATGATTACTGTGGCTGCTTTTTTTGCACCGCTAATAAACGAATCAGCACGCTCGTCTATCACAC  >  CP006881/2187824‑2188079                                                                                                                              |                                                                                                                                   gCCACTCCTGATTTATGTGAACCCGCCATCGGATGCCCTCCGACACATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTc                                                                                                                       <  1:259361/139‑1 (MQ=255)               ttgtgAACCCGCCATCGGATGCCATCCGACAATTTGATAGCGGCTAGGCAGCACTTGATGAGCGTAATCAACCAGTTTTTGCTTTGTGCTTCCTACATCCGTTATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAg                                                                                                          <  1:204922/137‑1 (MQ=255)               aTGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAg                                                                                                          <  4:40126/138‑1 (MQ=255)               aTGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAg                                                                                                          <  8:239858/138‑1 (MQ=255)               aTGTGAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAg                                                                                                          <  2:176303/138‑1 (MQ=255)                tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTc                                                                                                                            <  7:177986/119‑1 (MQ=255)                tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTc                                                                                                                            >  8:177986/1‑119 (MQ=255)                tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGc                                                                                                         >  8:408078/1‑138 (MQ=255)                tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCt                                                                                                        >  4:368384/1‑139 (MQ=255)                tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCt                                                                                                        >  6:377443/1‑139 (MQ=255)                tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCt                                                                                                        >  3:180037/1‑139 (MQ=255)                tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCt                                                                                                        >  7:445073/1‑139 (MQ=255)                tgtgAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCt                                                                                                        >  2:344272/1‑139 (MQ=255)                  tgAACCCGCCATCGGATGCCCTCCGACAAATTTATAGCGGCTAGGCTGCACTTGATCAGCGTAATGAAACACTTTTTGCGTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCATATCCTGAATGAGCCAGCtct                                                                                                      >  5:150840/1‑138 (MQ=255)                   gAACCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCtctt                                                                                                     <  8:445073/139‑3 (MQ=255)                    aaCCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCGTGAATGAGCCAg                                                                                                          <  6:384754/133‑1 (MQ=255)                    aaCCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCtctt                                                                                                     <  2:236594/138‑3 (MQ=255)                    aaCCCGCCATCGGATGCACTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAg                                                                                                          >  5:384754/1‑133 (MQ=255)                     aCCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCtcttc                                                                                                    >  7:68269/1‑138 (MQ=255)                     aCCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCtcttc                                                                                                    >  7:360087/1‑138 (MQ=255)                         gCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagca                                                                                               <  7:430800/139‑1 (MQ=255)                          ccATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagca                                                                                               >  4:340449/1‑138 (MQ=255)                          ccATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagca                                                                                               >  6:291034/1‑138 (MQ=255)                          ccATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagaa                                                                                               >  2:85703/1‑136 (MQ=255)                          ccATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATAAACCACTTTTTGCTTTGTGCTTCCTACAACCGTAATCAAAAGATCCTGTTCAATTCCTGAATGAGCCAGCTCTTCaagca                                                                                               >  6:56721/1‑138 (MQ=255)                               ggATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataacc                                                                                          <  8:31927/138‑3 (MQ=255)                               ggATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataacc                                                                                          <  5:90449/138‑3 (MQ=255)                                 aTGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagt                                                                                       <  2:229146/139‑3 (MQ=255)                                 aTGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagt                                                                                       <  5:377443/139‑3 (MQ=255)                                   gCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATACGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagaataaccagtgt                                                                                     >  4:101259/1‑135 (MQ=255)                                    ccctccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCtagcataaccagtgt                                                                                     <  1:265202/138‑5 (MQ=255)                                    ccctccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagtgtt                                                                                    <  8:360087/139‑6 (MQ=255)                                    ccctccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagtgtt                                                                                    >  2:166943/1‑134 (MQ=255)                                    ccctccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagtgt                                                                                     <  7:408078/138‑5 (MQ=255)                                       tccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTgt                                                                                 >  6:419618/2‑138 (MQ=255)                                         cGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACGACTTTTTGCTTTGTGCTTCCTACATACGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTgtt                                                                                <  3:75572/138‑3 (MQ=255)                                           aCAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTgttcaa                                                                             >  7:102544/1‑134 (MQ=255)                                           aCAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTgttcaa                                                                             <  5:11196/139‑6 (MQ=255)                                            cAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAAc                                                                            <  4:115761/139‑1 (MQ=255)                                               aTTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAgg                                                                         >  6:198446/1‑139 (MQ=255)                                               aTTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAgg                                                                         >  6:414869/1‑139 (MQ=255)                                                ttGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGgt                                                                        >  4:224357/1‑139 (MQ=255)                                                   atagcGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaag                                                                                                 <  5:355217/107‑1 (MQ=255)                                                   atagcGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaag                                                                                                 >  6:355217/5‑111 (MQ=255)                                                   atagcGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGttgtc                                                                     >  2:442764/5‑138 (MQ=255)                                                     agcGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGcc                                                                                                            <  2:174029/96‑1 (MQ=255)                                                     agcGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGcc                                                                                                            >  1:174029/3‑98 (MQ=255)                                                      gcGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCa                                                                  <  8:105590/138‑1 (MQ=255)                                                      gcGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCa                                                                  <  8:69353/138‑1 (MQ=255)                                                             ggCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGAt                                                             <  3:224357/137‑1 (MQ=255)                                                             ggCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGAt                                                             <  8:149749/137‑1 (MQ=255)                                                                  caCTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACtg                                                        >  3:51695/1‑137 (MQ=255)                                                                  caCTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACta                                                        >  3:404156/1‑136 (MQ=255)                                                                  caCTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTAATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCATCAGGTGTCGCTATGATTACtgta                                                      >  2:77284/1‑138 (MQ=255)                                                                  caCTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAACCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTCTTCAACAGGTGTCGCAATGATTACtg                                                        >  5:73843/1‑137 (MQ=255)                                                                   aCTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACtgtag                                                     <  5:419618/139‑3 (MQ=255)                                                                       gatCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTa                                                           <  3:256247/127‑1 (MQ=255)                                                                       gatCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTa                                                           >  4:256247/3‑129 (MQ=255)                                                                       gatCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgctgc                                                 >  4:21532/3‑139 (MQ=255)                                                                       gatCAGCGTAATCAACCACTTATTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgctgc                                                 <  5:35394/137‑1 (MQ=255)                                                                        atCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgctgc                                                 <  3:21532/137‑1 (MQ=255)                                                                        atCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgctgc                                                 <  4:51695/137‑1 (MQ=255)                                                                               taatcaaCCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCa                                                                  <  3:59820/109‑1 (MQ=255)                                                                               taatcaaCCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCa                                                                  >  4:59820/6‑114 (MQ=255)                                                                                       cACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCt                                  >  2:72403/1‑138 (MQ=255)                                                                                          tttttGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCCCGCATACCCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCtaat                               >  8:30112/1‑138 (MQ=255)                                                                                            tttGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATaaa                            >  2:56631/1‑139 (MQ=255)                                                                                             ttGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTACTGAATGAGCCAGCTCTTCAAGAATAACCAGTGTTTGTTCAACAGGTGTCGAAATGATTACTTTAGCTGCCTCTTTCACACCGCTAATaaa                            <  1:85703/138‑1 (MQ=255)                                                                                                   tgtgCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCa                     >  2:87486/1‑139 (MQ=255)                                                                                                     tgCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAg                    <  4:251910/138‑1 (MQ=255)                                                                                                       cTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCa                  <  1:87486/138‑1 (MQ=255)                                                                                                       cTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCa                  <  8:148402/138‑1 (MQ=255)                                                                                                              cATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGt           <  2:257463/138‑1 (MQ=255)                                                                                                               aTCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgctgc                                                 >  7:340308/1‑99 (MQ=255)                                                                                                               aTCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgctgc                                                 <  8:340308/99‑1 (MQ=255)                                                                                                                 ccgTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCGCACCGCTAATAAACTAATCAGCACGATCGTCTAt       >  5:368440/3‑139 (MQ=255)                                                                                                                 ccgTAATCAAAAGCACATGTTCAATTCCTGAATGAGCCAGCTCTGCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTCCTGAAGATGCCTCTTTCACACCGCTAAAAAACGATGCAGAACGCACGTCtct       >  6:30446/3‑137 (MQ=255)                                                                                                                    tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAAc                                                                            <  6:429333/67‑1 (MQ=255)                                                                                                                    tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAAc                                                                            <  2:187461/67‑1 (MQ=255)                                                                                                                    tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAAc                                                                            >  5:429333/1‑67 (MQ=255)                                                                                                                    tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAAc                                                                            >  1:187461/1‑67 (MQ=255)                                                                                                                    tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgc                                                    <  3:291017/91‑1 (MQ=255)                                                                                                                    tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgc                                                    >  4:291017/1‑91 (MQ=255)                                                                                                                    tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcac    >  5:132904/1‑139 (MQ=255)                                                                                                                    tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcac    >  1:231024/1‑139 (MQ=255)                                                                                                                    tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcac    >  8:434852/1‑139 (MQ=255)                                                                                                                    tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcac    >  1:151645/1‑139 (MQ=255)                                                                                                                      aTCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcacgc  >  1:41200/1‑137 (MQ=255)                                                                                                                              |                                                                                                                                   GCCACTCCTGATTTATGTGAACCTGCCATCGGATGCCCCCCGACAAATTGGTATCGGCCAGGCAGCACTTGCTCAGCGTATCGAACCACTTTTTGCTTTGTGCTTCCTACATCGGTAATCAAAAGTTGATGTTCAATTCCTGAATGAGCCAGCTCATCAAGCATAAGCATGGTTTGCGCAACAGGTGTGGCAATGATTACTGTGGCTGCTTTTTTTGCACCGCTAATAAACGAATCAGCACGCTCGTCTATCACAC  >  CP006881/2187824‑2188079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑