Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,187,979	A→T	D83E (GAT→GAA)	U712_10960 ←	Prephenate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,187,979	0	A	T	100.0%	260.9 / NA	82	D83E (GAT→GAA)	U712_10960	Prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (45/37);  total (45/37)

ACCTGCCATCGGATGCCCCCCGACAAATTGGTATCGGCCAGGCAGCACTTGCTCAGCGTATCGAACCACTTTTTGCTTTGTGCTTCCTACATCGGTAATCAAAAGTTGATGTTCAATTCCTGAATGAGCCAGCTCATCAAGCATAAGCATGGTTTGCGCAACAGGTGTGGCAATGATTACTGTGGCTGCTTTTTTTGCACCGCTAATAAACGAATCAGCACGCTCGTCTATCACACCTAATTTTAATGCGGCTACTACCTGTTCATCAGA  >  CP006881/2187844‑2188113                                                                                                                                        |                                                                                                                                       aCCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCtcttc                                                                                                                                      >  7:68269/1‑138 (MQ=255) aCCCGCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCtcttc                                                                                                                                      >  7:360087/1‑138 (MQ=255)     gCCATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagca                                                                                                                                 <  7:430800/139‑1 (MQ=255)      ccATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagca                                                                                                                                 >  6:291034/1‑138 (MQ=255)      ccATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagca                                                                                                                                 >  4:340449/1‑138 (MQ=255)      ccATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagaa                                                                                                                                 >  2:85703/1‑136 (MQ=255)      ccATCGGATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATAAACCACTTTTTGCTTTGTGCTTCCTACAACCGTAATCAAAAGATCCTGTTCAATTCCTGAATGAGCCAGCTCTTCaagca                                                                                                                                 >  6:56721/1‑138 (MQ=255)           ggATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataacc                                                                                                                            <  8:31927/138‑3 (MQ=255)           ggATGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataacc                                                                                                                            <  5:90449/138‑3 (MQ=255)             aTGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagt                                                                                                                         <  2:229146/139‑3 (MQ=255)             aTGCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagt                                                                                                                         <  5:377443/139‑3 (MQ=255)               gCCCTCCGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATACGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagaataaccagtgt                                                                                                                       >  4:101259/1‑135 (MQ=255)                ccctccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCtagcataaccagtgt                                                                                                                       <  1:265202/138‑5 (MQ=255)                ccctccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagtgtt                                                                                                                      >  2:166943/1‑134 (MQ=255)                ccctccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagtgtt                                                                                                                      <  8:360087/139‑6 (MQ=255)                ccctccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaagcataaccagtgt                                                                                                                       <  7:408078/138‑5 (MQ=255)                   tccGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTgt                                                                                                                   >  6:419618/2‑138 (MQ=255)                     cGACAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACGACTTTTTGCTTTGTGCTTCCTACATACGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTgtt                                                                                                                  <  3:75572/138‑3 (MQ=255)                       aCAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTgttcaa                                                                                                               <  5:11196/139‑6 (MQ=255)                       aCAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTgttcaa                                                                                                               >  7:102544/1‑134 (MQ=255)                        cAAATTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAAc                                                                                                              <  4:115761/139‑1 (MQ=255)                           aTTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAgg                                                                                                           >  6:198446/1‑139 (MQ=255)                           aTTGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAgg                                                                                                           >  6:414869/1‑139 (MQ=255)                            ttGATAGCGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGgt                                                                                                          >  4:224357/1‑139 (MQ=255)                               atagcGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaag                                                                                                                                   >  6:355217/5‑111 (MQ=255)                               atagcGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCaag                                                                                                                                   <  5:355217/107‑1 (MQ=255)                               atagcGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGttgtc                                                                                                       >  2:442764/5‑138 (MQ=255)                                  gcGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCa                                                                                                    <  8:105590/138‑1 (MQ=255)                                  gcGGCTAGGCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCa                                                                                                    <  8:69353/138‑1 (MQ=255)                                         ggCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGAt                                                                                               <  8:149749/137‑1 (MQ=255)                                         ggCAGCACTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGAt                                                                                               <  3:224357/137‑1 (MQ=255)                                              caCTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACtg                                                                                          >  3:51695/1‑137 (MQ=255)                                              caCTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACta                                                                                          >  3:404156/1‑136 (MQ=255)                                              caCTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTAATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCATCAGGTGTCGCTATGATTACtgta                                                                                        >  2:77284/1‑138 (MQ=255)                                              caCTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAACCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTCTTCAACAGGTGTCGCAATGATTACtg                                                                                          >  5:73843/1‑137 (MQ=255)                                               aCTTGATCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACtgtag                                                                                       <  5:419618/139‑3 (MQ=255)                                                   gatCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTa                                                                                             >  4:256247/3‑129 (MQ=255)                                                   gatCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTa                                                                                             <  3:256247/127‑1 (MQ=255)                                                   gatCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgctgc                                                                                   >  4:21532/3‑139 (MQ=255)                                                   gatCAGCGTAATCAACCACTTATTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgctgc                                                                                   <  5:35394/137‑1 (MQ=255)                                                    atCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgctgc                                                                                   <  3:21532/137‑1 (MQ=255)                                                    atCAGCGTAATCAACCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgctgc                                                                                   <  4:51695/137‑1 (MQ=255)                                                           taatcaaCCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCa                                                                                                    <  3:59820/109‑1 (MQ=255)                                                           taatcaaCCACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCa                                                                                                    >  4:59820/6‑114 (MQ=255)                                                                   cACTTTTTGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCt                                                                    >  2:72403/1‑138 (MQ=255)                                                                      tttttGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCCCGCATACCCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCtaat                                                                 >  8:30112/1‑138 (MQ=255)                                                                        tttGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATaaa                                                              >  2:56631/1‑139 (MQ=255)                                                                         ttGCTTTGTGCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTACTGAATGAGCCAGCTCTTCAAGAATAACCAGTGTTTGTTCAACAGGTGTCGAAATGATTACTTTAGCTGCCTCTTTCACACCGCTAATaaa                                                              <  1:85703/138‑1 (MQ=255)                                                                               tgtgCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCa                                                       >  2:87486/1‑139 (MQ=255)                                                                                 tgCTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAg                                                      <  4:251910/138‑1 (MQ=255)                                                                                   cTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCa                                                    <  1:87486/138‑1 (MQ=255)                                                                                   cTTCCTACATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCa                                                    <  8:148402/138‑1 (MQ=255)                                                                                          cATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGt                                             <  2:257463/138‑1 (MQ=255)                                                                                           aTCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgctgc                                                                                   >  7:340308/1‑99 (MQ=255)                                                                                           aTCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgctgc                                                                                   <  8:340308/99‑1 (MQ=255)                                                                                             ccgTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCGCACCGCTAATAAACTAATCAGCACGATCGTCTAt                                         >  5:368440/3‑139 (MQ=255)                                                                                             ccgTAATCAAAAGCACATGTTCAATTCCTGAATGAGCCAGCTCTGCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTCCTGAAGATGCCTCTTTCACACCGCTAAAAAACGATGCAGAACGCACGTCtct                                         >  6:30446/3‑137 (MQ=255)                                                                                                tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAAc                                                                                                              >  5:429333/1‑67 (MQ=255)                                                                                                tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAAc                                                                                                              <  6:429333/67‑1 (MQ=255)                                                                                                tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAAc                                                                                                              >  1:187461/1‑67 (MQ=255)                                                                                                tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAAc                                                                                                              <  2:187461/67‑1 (MQ=255)                                                                                                tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgc                                                                                      <  3:291017/91‑1 (MQ=255)                                                                                                tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAgc                                                                                      >  4:291017/1‑91 (MQ=255)                                                                                                tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcac                                      >  1:151645/1‑139 (MQ=255)                                                                                                tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcac                                      >  8:434852/1‑139 (MQ=255)                                                                                                tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcac                                      >  5:132904/1‑139 (MQ=255)                                                                                                tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcac                                      >  1:231024/1‑139 (MQ=255)                                                                                                  aTCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcacgc                                    >  1:41200/1‑137 (MQ=255)                                                                                                       aagctcaTGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAAt                               >  5:165699/7‑139 (MQ=255)                                                                                                       aagctcaTGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAAt                               >  8:228725/7‑139 (MQ=255)                                                                                                       aagctcaTGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAAt                               >  8:234891/7‑139 (MQ=255)                                                                                                                   aaTTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCTATGATTAGAGTAGCTGCCTCTTTCACACCTCTAATAAACGATTCAGCACGATAGTCTATCACGCCTAATTTTAATGCCGct                   <  8:387100/139‑1 (MQ=255)                                                                                                                     ttCCTGAATGAGCCAGCTCTTCAAGCATAGCCAGTGATTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGATTCAGCACGATCGTCTATCACGCCTAATTTTATTGCCGatac                 >  6:56034/1‑139 (MQ=255)                                                                                                                       ccTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGctacc                <  8:68269/138‑2 (MQ=255)                                                                                                                         tGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGctaccgcc             >  3:76958/1‑135 (MQ=255)                                                                                                                           aaTGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTg           <  3:340449/139‑1 (MQ=255)                                                                                                                            aTGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTg           <  5:198446/138‑1 (MQ=255)                                                                                                                               aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCTCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCAGCTACCGCCTGTtct       >  7:120220/1‑138 (MQ=255)                                                                                                                               aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCGTGTtca       >  6:92610/1‑139 (MQ=255)                                                                                                                               aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTtc        >  3:356185/1‑138 (MQ=255)                                                                                                                               aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCAGCTACCGCCTGTtc        >  3:436173/1‑138 (MQ=255)                                                                                                                               aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCCCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTtca       >  1:362833/1‑139 (MQ=255)                                                                                                                                    gCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCaga  <  1:344272/139‑1 (MQ=255)                                                                                                                                    gCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCaga  <  2:231024/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       ACCTGCCATCGGATGCCCCCCGACAAATTGGTATCGGCCAGGCAGCACTTGCTCAGCGTATCGAACCACTTTTTGCTTTGTGCTTCCTACATCGGTAATCAAAAGTTGATGTTCAATTCCTGAATGAGCCAGCTCATCAAGCATAAGCATGGTTTGCGCAACAGGTGTGGCAATGATTACTGTGGCTGCTTTTTTTGCACCGCTAATAAACGAATCAGCACGCTCGTCTATCACACCTAATTTTAATGCGGCTACTACCTGTTCATCAGA  >  CP006881/2187844‑2188113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑