Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,188,066	C→A	E54D (GAG→GAT)	U712_10960 ←	Prephenate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,188,066	0	C	A	96.8%	274.0 / ‑3.9	93	E54D (GAG→GAT)	U712_10960	Prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (51/39);  total (53/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCGGTAATCAAAAGTTGATGTTCAATTCCTGAATGAGCCAGCTCATCAAGCATAAGCATGGTTTGCGCAACAGGTGTGGCAATGATTACTGTGGCTGCTTTTTTTGCACCGCTAATAAACGAATCAGCACGCTCGTCTATCACACCTAATTTTAATGCGGCTACTACCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCCGGATGCTCTTTTTTGATCGCTAGCGCAATCGAACCGCCTATCAATCCGAGACCGGCGAGC  >  CP006881/2187933‑2188201                                                                                                                                      |                                                                                                                                        cATCCGTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGt                                                                                                                                     <  2:257463/138‑1 (MQ=255)    ccgTAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCGCACCGCTAATAAACTAATCAGCACGATCGTCTAt                                                                                                                                 >  5:368440/3‑139 (MQ=255)    ccgTAATCAAAAGCACATGTTCAATTCCTGAATGAGCCAGCTCTGCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTCCTGAAGATGCCTCTTTCACACCGCTAAAAAACGATGCAGAACGCACGTCtct                                                                                                                                 >  6:30446/3‑137 (MQ=255)       tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcac                                                                                                                              >  1:231024/1‑139 (MQ=255)       tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcac                                                                                                                              >  8:434852/1‑139 (MQ=255)       tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcac                                                                                                                              >  1:151645/1‑139 (MQ=255)       tAATCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcac                                                                                                                              >  5:132904/1‑139 (MQ=255)         aTCAAAAGCTCATGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATcacgc                                                                                                                            >  1:41200/1‑137 (MQ=255)              aagctcaTGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAAt                                                                                                                       >  8:234891/7‑139 (MQ=255)              aagctcaTGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAAt                                                                                                                       >  5:165699/7‑139 (MQ=255)              aagctcaTGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAAt                                                                                                                       >  8:228725/7‑139 (MQ=255)                          aaTTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCTATGATTAGAGTAGCTGCCTCTTTCACACCTCTAATAAACGATTCAGCACGATAGTCTATCACGCCTAATTTTAATGCCGct                                                                                                           <  8:387100/139‑1 (MQ=255)                            ttCCTGAATGAGCCAGCTCTTCAAGCATAGCCAGTGATTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGATTCAGCACGATCGTCTATCACGCCTAATTTTATTGCCGatac                                                                                                         >  6:56034/1‑139 (MQ=255)                              ccTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGctacc                                                                                                        <  8:68269/138‑2 (MQ=255)                                tGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGctaccgcc                                                                                                     >  3:76958/1‑135 (MQ=255)                                  aaTGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTg                                                                                                   <  3:340449/139‑1 (MQ=255)                                   aTGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTg                                                                                                   <  5:198446/138‑1 (MQ=255)                                      aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCTCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCAGCTACCGCCTGTtct                                                                                               >  7:120220/1‑138 (MQ=255)                                      aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCGTGTtca                                                                                               >  6:92610/1‑139 (MQ=255)                                      aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTtc                                                                                                >  3:356185/1‑138 (MQ=255)                                      aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCAGCTACCGCCTGTtc                                                                                                >  3:436173/1‑138 (MQ=255)                                      aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCCCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTtca                                                                                               >  1:362833/1‑139 (MQ=255)                                           gCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCaga                                                                                          <  1:344272/139‑1 (MQ=255)                                           gCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCaga                                                                                          <  2:231024/139‑1 (MQ=255)                                                 caAGCATAACCAGTGTTTGTTCAACAGGTCTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACTAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTc                                                                                    >  5:328561/1‑139 (MQ=255)                                                   agcataaccagTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGa                                                                                  <  6:165699/139‑1 (MQ=255)                                                    gcataaccagTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGAt                                                                                 <  4:180037/139‑1 (MQ=255)                                                     cataaccagTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCt                                                                                                                          >  5:248493/1‑97 (MQ=255)                                                     cataaccagTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCt                                                                                                                          <  6:248493/97‑1 (MQ=255)                                                         accagtgTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGa                                                                                  <  6:79095/127‑1 (MQ=255)                                                         accagtgTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGa                                                                                  >  5:79095/7‑133 (MQ=255)                                                         accagtgTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCg                                                                             >  8:228619/7‑138 (MQ=255)                                                         accagtgTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGa                                                                            >  4:99306/7‑139 (MQ=255)                                                                  ttttcAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACAACGCTAATAAACGAATCAGCACCATCGTCTATCACGCCTAATTTTAATCCCGCAAACGCCACTTCATAAGACATGTCGATTGCCATAATCCGtt                                                                   >  7:118568/5‑139 (MQ=255)                                                                  tgttcAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGtt                                                                   >  5:438272/5‑139 (MQ=255)                                                                    ttcAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCTTTTg                                                                 >  6:379106/3‑139 (MQ=255)                                                                    ttcAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTg                                                                 >  5:335068/3‑139 (MQ=255)                                                                     tcAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGc                                                                >  4:79419/2‑139 (MQ=255)                                                                              gtcgCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATgat                                                       >  4:196138/4‑137 (MQ=255)                                                                              gtcgCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATgat                                                       >  4:327787/4‑137 (MQ=255)                                                                                      gagtACTGTAGCTGCCTCTTTCACACCGCTAAGAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGAGTCCGATTATCCGTTTGCCGGGATGATTTTTTTTg                                               <  5:56034/136‑1 (MQ=255)                                                                                       aTTACTTTATCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACTCTCGTCTATCACGCCTAATTTTAAAGCCGCTACCGCCTGTTCATCCGAGATGTCGATTCCGATAATCCGTTTTCAGGGATGATTTTTTTTg                                               <  6:73843/138‑1 (MQ=255)                                                                                       aTTACTGTGGCTGCTTTTTTTGCACCGCTAATAAACGAATCAGCACGCTCGTCTATCACACCTAATTTTAATGCGGCTACTACCTGTTCATCAGAGATGTCGATTCAGATAATCCGTTTGCCCGGATGCTCTTTTTTGa                                              >  6:344632/1‑139 (MQ=255)                                                                                       aTTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTg                                               <  1:442764/138‑1 (MQ=255)                                                                                       aTTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTg                                               <  8:240969/138‑1 (MQ=255)                                                                                       aTTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTATGa                                              >  1:437823/1‑139 (MQ=255)                                                                                       aTTACTGTAGCTGCCTCTTTCACACAGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTTATTTTAATGCCGCTTCCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTg                                               <  8:118568/138‑1 (MQ=255)                                                                                       aTTACTGAAGCTGACTCTTTCACACCTCTAATATACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCGGCTACAGGCTGTTCATCAGAGATGTCGATTCCGATAATGCGTTTGCCGGGTTGATTTTTTTTg                                               <  4:356185/138‑1 (MQ=255)                                                                                         tACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTg                                               >  8:393995/1‑136 (MQ=255)                                                                                         tACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTg                                               <  7:393995/136‑1 (MQ=255)                                                                                           atgtaGCTGCCTCTTTCACACCGCTAATAAACGAAGCAGCACGATCGTCTATCACGCCTAATTTTACTGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTGTGCCGGGATGATTTTTTTTGATGGc                                          <  6:368440/138‑1 (MQ=255)                                                                                             gtagctgccTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGc                                          >  8:196784/4‑137 (MQ=255)                                                                                             gtagctgccTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGc                                          >  7:282254/4‑137 (MQ=255)                                                                                                gctgccTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAggg                                     >  4:389056/1‑137 (MQ=255)                                                                                                gctgccTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAggg                                     >  3:289844/1‑137 (MQ=255)                                                                                                gctgccTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAggg                                     >  7:306275/1‑137 (MQ=255)                                                                                                          tcacACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGaa                             <  2:401862/134‑1 (MQ=255)                                                                                                           cacACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAAcc                           <  4:76958/136‑1 (MQ=255)                                                                                                              aCCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGcc                        >  5:12672/1‑138 (MQ=255)                                                                                                                cGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCg                                                                             <  1:91799/83‑1 (MQ=255)                                                                                                                cGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCg                                                                             >  2:91799/1‑83 (MQ=255)                                                                                                                cGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTAt                     >  6:50411/1‑139 (MQ=255)                                                                                                                cGCTAATAAACGAATCAGCACGATCGTCTATCAAGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTAt                     <  4:404156/139‑1 (MQ=255)                                                                                                                  cTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATg                                                                                                                <  1:2247/46‑1 (MQ=255)                                                                                                                  cTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATg                                                                                                                >  2:2247/1‑46 (MQ=255)                                                                                                                  cTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGaa                             <  1:284979/129‑1 (MQ=255)                                                                                                                  cTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGaa                             >  4:134042/1‑129 (MQ=255)                                                                                                                  cTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGaa                             >  2:284979/1‑129 (MQ=255)                                                                                                                  cTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGaa                             <  3:134042/129‑1 (MQ=255)                                                                                                                          cGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGa                              >  5:226429/1‑120 (MQ=255)                                                                                                                          cGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGa                              <  6:226429/120‑1 (MQ=255)                                                                                                                          cGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCgaga           >  6:198397/1‑139 (MQ=255)                                                                                                                            aaTCAGCACGATCTTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATAAGAGATGTCGATTCCGATAATCCGTTATCCGTGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCATATAAATCAGAGAcc         >  4:336827/1‑139 (MQ=255)                                                                                                                            aaTCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGAcc         >  5:313158/1‑139 (MQ=255)                                                                                                                             aTCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGCCCg        >  4:2988/1‑139 (MQ=255)                                                                                                                             aTCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCg        >  5:203777/1‑139 (MQ=255)                                                                                                                             aTCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCg        >  1:291839/1‑139 (MQ=255)                                                                                                                             aTCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTAGCAATCCGAGACCg        >  2:330283/1‑139 (MQ=255)                                                                                                                             aTCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTAACGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGAAATCGAACCGCCTATCAATCCCAGACCg        >  7:161799/1‑139 (MQ=255)                                                                                                                               cAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGc      <  7:434852/139‑1 (MQ=255)                                                                                                                                aGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCTTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGCACCGCCTATCAATCCGAGACCGGc      <  5:379106/138‑1 (MQ=255)                                                                                                                                aGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGc      <  3:24698/138‑1 (MQ=255)                                                                                                                                aGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGAGGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCg     <  5:198397/139‑1 (MQ=255)                                                                                                                                aGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATAAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGc      >  5:396890/1‑138 (MQ=255)                                                                                                                                  cACGATCGTCTATCACTCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg   <  3:336827/139‑1 (MQ=255)                                                                                                                                  cACGATCGTCTATCACGCCTAATTTTAATGCCGGTTCCGCCTGTTCATCAGTGATGTCGATTCCGTTAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg   <  1:177932/139‑1 (MQ=255)                                                                                                                                  cACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg   <  5:414869/139‑1 (MQ=255)                                                                                                                                  cACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg   <  3:327787/139‑1 (MQ=255)                                                                                                                                  cACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg   <  7:330457/139‑1 (MQ=255)                                                                                                                                  cACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg   <  6:132904/139‑1 (MQ=255)                                                                                                                                  cACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg   <  7:30112/139‑1 (MQ=255)                                                                                                                                  aaCGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCGTGTTCATCTTAGATGTCGATTCCGTTAATCCGTTTGCCGTGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg   <  6:396890/138‑1 (MQ=255)                                                                                                                                   aCGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAGc  <  3:196138/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        CATCGGTAATCAAAAGTTGATGTTCAATTCCTGAATGAGCCAGCTCATCAAGCATAAGCATGGTTTGCGCAACAGGTGTGGCAATGATTACTGTGGCTGCTTTTTTTGCACCGCTAATAAACGAATCAGCACGCTCGTCTATCACACCTAATTTTAATGCGGCTACTACCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCCGGATGCTCTTTTTTGATCGCTAGCGCAATCGAACCGCCTATCAATCCGAGACCGGCGAGC  >  CP006881/2187933‑2188201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑