Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,188,078 A→G G50G (GGT→GGC)  U712_10960 ← Prephenate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,188,0780AG98.9% 275.2 / ‑4.9 88G50G (GGT→GGC) U712_10960Prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (45/41);  total (46/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

AAGTTGATGTTCAATTCCTGAATGAGCCAGCTCATCAAGCATAAGCATGGTTTGCGCAACAGGTGTGGCAATGATTACTGTGGCTGCTTTTTTTGCACCGCTAATAAACGAATCAGCACGCTCGTCTATCACACCTAATTTTAATGCGGCTACTACCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCCGGATGCTCTTTTTTGATCGCTAGCGCAATCGAACCGCCTATCAATCCGAGACCGGCGAGCAATATTGTATCT  >  CP006881/2187946‑2188213
                                                                                                                                    |                                                                                                                                       
aagctcaTGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAAt                                                                                                                                   >  8:234891/7‑139 (MQ=255)
aagctcaTGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAAt                                                                                                                                   >  5:165699/7‑139 (MQ=255)
aagctcaTGTTCAATTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAAt                                                                                                                                   >  8:228725/7‑139 (MQ=255)
            aaTTCCTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCTATGATTAGAGTAGCTGCCTCTTTCACACCTCTAATAAACGATTCAGCACGATAGTCTATCACGCCTAATTTTAATGCCGct                                                                                                                       <  8:387100/139‑1 (MQ=255)
              ttCCTGAATGAGCCAGCTCTTCAAGCATAGCCAGTGATTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGATTCAGCACGATCGTCTATCACGCCTAATTTTATTGCCGatac                                                                                                                     >  6:56034/1‑139 (MQ=255)
                ccTGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGctacc                                                                                                                    <  8:68269/138‑2 (MQ=255)
                  tGAATGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGctaccgcc                                                                                                                 >  3:76958/1‑135 (MQ=255)
                    aaTGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTg                                                                                                               <  3:340449/139‑1 (MQ=255)
                     aTGAGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTg                                                                                                               <  5:198446/138‑1 (MQ=255)
                        aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCTCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCAGCTACCGCCTGTtct                                                                                                           >  7:120220/1‑138 (MQ=255)
                        aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCGTGTtca                                                                                                           >  6:92610/1‑139 (MQ=255)
                        aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTtc                                                                                                            >  3:356185/1‑138 (MQ=255)
                        aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCAGCTACCGCCTGTtc                                                                                                            >  3:436173/1‑138 (MQ=255)
                        aGCCAGCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCCCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTtca                                                                                                           >  1:362833/1‑139 (MQ=255)
                             gCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCaga                                                                                                      <  1:344272/139‑1 (MQ=255)
                             gCTCTTCAAGCATAACCAGTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCaga                                                                                                      <  2:231024/139‑1 (MQ=255)
                                   caAGCATAACCAGTGTTTGTTCAACAGGTCTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACTAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTc                                                                                                >  5:328561/1‑139 (MQ=255)
                                     agcataaccagTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGa                                                                                              <  6:165699/139‑1 (MQ=255)
                                      gcataaccagTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGAt                                                                                             <  4:180037/139‑1 (MQ=255)
                                       cataaccagTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCt                                                                                                                                      >  5:248493/1‑97 (MQ=255)
                                       cataaccagTGTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCt                                                                                                                                      <  6:248493/97‑1 (MQ=255)
                                           accagtgTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGa                                                                                              <  6:79095/127‑1 (MQ=255)
                                           accagtgTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGa                                                                                              >  5:79095/7‑133 (MQ=255)
                                           accagtgTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCg                                                                                         >  8:228619/7‑138 (MQ=255)
                                           accagtgTTTGTTCAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGa                                                                                        >  4:99306/7‑139 (MQ=255)
                                                    ttttcAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACAACGCTAATAAACGAATCAGCACCATCGTCTATCACGCCTAATTTTAATCCCGCAAACGCCACTTCATAAGACATGTCGATTGCCATAATCCGtt                                                                               >  7:118568/5‑139 (MQ=255)
                                                    tgttcAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGtt                                                                               >  5:438272/5‑139 (MQ=255)
                                                      ttcAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCTTTTg                                                                             >  6:379106/3‑139 (MQ=255)
                                                      ttcAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTg                                                                             >  5:335068/3‑139 (MQ=255)
                                                       tcAACAGGTGTCGCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGc                                                                            >  4:79419/2‑139 (MQ=255)
                                                                gtcgCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATgat                                                                   >  4:327787/4‑137 (MQ=255)
                                                                gtcgCAATGATTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATgat                                                                   >  4:196138/4‑137 (MQ=255)
                                                                        gagtACTGTAGCTGCCTCTTTCACACCGCTAAGAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGAGTCCGATTATCCGTTTGCCGGGATGATTTTTTTTg                                                           <  5:56034/136‑1 (MQ=255)
                                                                         aTTACTTTATCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACTCTCGTCTATCACGCCTAATTTTAAAGCCGCTACCGCCTGTTCATCCGAGATGTCGATTCCGATAATCCGTTTTCAGGGATGATTTTTTTTg                                                           <  6:73843/138‑1 (MQ=255)
                                                                         aTTACTGTGGCTGCTTTTTTTGCACCGCTAATAAACGAATCAGCACGCTCGTCTATCACACCTAATTTTAATGCGGCTACTACCTGTTCATCAGAGATGTCGATTCAGATAATCCGTTTGCCCGGATGCTCTTTTTTGa                                                          >  6:344632/1‑139 (MQ=255)
                                                                         aTTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTg                                                           <  1:442764/138‑1 (MQ=255)
                                                                         aTTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTg                                                           <  8:240969/138‑1 (MQ=255)
                                                                         aTTACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTATGa                                                          >  1:437823/1‑139 (MQ=255)
                                                                         aTTACTGTAGCTGCCTCTTTCACACAGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTTATTTTAATGCCGCTTCCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTg                                                           <  8:118568/138‑1 (MQ=255)
                                                                         aTTACTGAAGCTGACTCTTTCACACCTCTAATATACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCGGCTACAGGCTGTTCATCAGAGATGTCGATTCCGATAATGCGTTTGCCGGGTTGATTTTTTTTg                                                           <  4:356185/138‑1 (MQ=255)
                                                                           tACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTg                                                           <  7:393995/136‑1 (MQ=255)
                                                                           tACTGTAGCTGCCTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTg                                                           >  8:393995/1‑136 (MQ=255)
                                                                             atgtaGCTGCCTCTTTCACACCGCTAATAAACGAAGCAGCACGATCGTCTATCACGCCTAATTTTACTGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTGTGCCGGGATGATTTTTTTTGATGGc                                                      <  6:368440/138‑1 (MQ=255)
                                                                               gtagctgccTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGc                                                      >  7:282254/4‑137 (MQ=255)
                                                                               gtagctgccTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGc                                                      >  8:196784/4‑137 (MQ=255)
                                                                                  gctgccTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAggg                                                 >  3:289844/1‑137 (MQ=255)
                                                                                  gctgccTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAggg                                                 >  7:306275/1‑137 (MQ=255)
                                                                                  gctgccTCTTTCACACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAggg                                                 >  4:389056/1‑137 (MQ=255)
                                                                                            tcacACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGaa                                         <  2:401862/134‑1 (MQ=255)
                                                                                             cacACCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAAcc                                       <  4:76958/136‑1 (MQ=255)
                                                                                                aCCGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGcc                                    >  5:12672/1‑138 (MQ=255)
                                                                                                  cGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCg                                                                                         <  1:91799/83‑1 (MQ=255)
                                                                                                  cGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCg                                                                                         >  2:91799/1‑83 (MQ=255)
                                                                                                  cGCTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTAt                                 >  6:50411/1‑139 (MQ=255)
                                                                                                  cGCTAATAAACGAATCAGCACGATCGTCTATCAAGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTAt                                 <  4:404156/139‑1 (MQ=255)
                                                                                                    cTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATg                                                                                                                            >  2:2247/1‑46 (MQ=255)
                                                                                                    cTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATg                                                                                                                            <  1:2247/46‑1 (MQ=255)
                                                                                                    cTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGaa                                         >  4:134042/1‑129 (MQ=255)
                                                                                                    cTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGaa                                         >  2:284979/1‑129 (MQ=255)
                                                                                                    cTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGaa                                         <  1:284979/129‑1 (MQ=255)
                                                                                                    cTAATAAACGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGaa                                         <  3:134042/129‑1 (MQ=255)
                                                                                                            cGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGa                                          >  5:226429/1‑120 (MQ=255)
                                                                                                            cGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGa                                          <  6:226429/120‑1 (MQ=255)
                                                                                                            cGAATCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCgaga                       >  6:198397/1‑139 (MQ=255)
                                                                                                              aaTCAGCACGATCTTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATAAGAGATGTCGATTCCGATAATCCGTTATCCGTGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCATATAAATCAGAGAcc                     >  4:336827/1‑139 (MQ=255)
                                                                                                              aaTCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGAcc                     >  5:313158/1‑139 (MQ=255)
                                                                                                               aTCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGCCCg                    >  4:2988/1‑139 (MQ=255)
                                                                                                               aTCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCg                    >  1:291839/1‑139 (MQ=255)
                                                                                                               aTCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCg                    >  5:203777/1‑139 (MQ=255)
                                                                                                               aTCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTAGCAATCCGAGACCg                    >  2:330283/1‑139 (MQ=255)
                                                                                                               aTCAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTAACGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGAAATCGAACCGCCTATCAATCCCAGACCg                    >  7:161799/1‑139 (MQ=255)
                                                                                                                 cAGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGc                  <  7:434852/139‑1 (MQ=255)
                                                                                                                  aGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCTTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGCACCGCCTATCAATCCGAGACCGGc                  <  5:379106/138‑1 (MQ=255)
                                                                                                                  aGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGc                  <  3:24698/138‑1 (MQ=255)
                                                                                                                  aGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGAGGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCg                 <  5:198397/139‑1 (MQ=255)
                                                                                                                  aGCACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATAAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGc                  >  5:396890/1‑138 (MQ=255)
                                                                                                                    cacgagcGTCTATCACGCCTAATTTTAAAGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg               <  1:166943/133‑1 (MQ=255)
                                                                                                                    cACGATCGTCTATCACTCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg               <  3:336827/139‑1 (MQ=255)
                                                                                                                    cACGATCGTCTATCACGCCTAATTTTAATGCCGGTTCCGCCTGTTCATCAGTGATGTCGATTCCGTTAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg               <  1:177932/139‑1 (MQ=255)
                                                                                                                    cACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg               <  7:330457/139‑1 (MQ=255)
                                                                                                                    cACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg               <  7:30112/139‑1 (MQ=255)
                                                                                                                    cACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg               <  5:414869/139‑1 (MQ=255)
                                                                                                                    cACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg               <  3:327787/139‑1 (MQ=255)
                                                                                                                    cACGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg               <  6:132904/139‑1 (MQ=255)
                                                                                                                    aaCGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCGTGTTCATCTTAGATGTCGATTCCGTTAATCCGTTTGCCGTGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAg               <  6:396890/138‑1 (MQ=255)
                                                                                                                     aCGATCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAGc              <  3:196138/139‑1 (MQ=255)
                                                                                                                      atttCGTGTATCACGCCTAATTTTAATGCCGCTACCTCCTTTTGATCATTGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACGGGCGAGCa             <  6:328561/136‑1 (MQ=255)
                                                                                                                         tCGTCTATCACGCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAGCAata          <  6:438272/139‑1 (MQ=255)
                                                                                                                                 cacgCCTAATTTTAATGCCGCTACCGCCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCGGGATGATTTTTTTTGATGGCTAGGGCAATCGAACCGCCTATCAATCCGAGACCGGCGAGCAATATTGTATCt  >  4:215503/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                       
AAGTTGATGTTCAATTCCTGAATGAGCCAGCTCATCAAGCATAAGCATGGTTTGCGCAACAGGTGTGGCAATGATTACTGTGGCTGCTTTTTTTGCACCGCTAATAAACGAATCAGCACGCTCGTCTATCACACCTAATTTTAATGCGGCTACTACCTGTTCATCAGAGATGTCGATTCCGATAATCCGTTTGCCCGGATGCTCTTTTTTGATCGCTAGCGCAATCGAACCGCCTATCAATCCGAGACCGGCGAGCAATATTGTATCT  >  CP006881/2187946‑2188213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑