Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,188,381 A→G I326I (ATT→ATC)  U712_10965 ← Histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,188,3810AG98.9% 289.5 / NA 89I326I (ATT→ATC) U712_10965Histidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (45/43);  minor base T (1/0);  total (46/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGACTAAAGAGGTGATATTTGATCACCTCTTATAAAATTTCAGCTAAAATTGCAAGAATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCATTTCCTGACCGGACAATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCGGAAGGGCGGTTAAAATCAATTAACACAAAGTTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTCTTCGCAAAATCATAATATTGCTGAAGGC  >  CP006881/2188246‑2188514
                                                                                                                                       |                                                                                                                                     
taGACTAAAGAGGTGATATTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGata                                                                                                                                    >  7:92592/1‑139 (MQ=255)
     tAAAGAGGTGATATTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGcg                                                                                                                               >  1:178434/1‑138 (MQ=255)
     tAAAGAGGTGATATTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGcc                                                                                                                               >  2:70056/1‑139 (MQ=255)
     tAAAGAGGTGATATTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTACTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGcc                                                                                                                               >  3:218567/1‑139 (MQ=255)
     tAAAGAGGTGAGATTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGcc                                                                                                                               >  1:66890/1‑139 (MQ=255)
      aaaGAGGTGATATTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCt                                                                                                                              >  4:5378/1‑139 (MQ=255)
       acgagGTGATATTTGATCACCTCTTATAAAATTTCAGCTAAACTGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCt                                                                                                                              <  4:297339/136‑1 (MQ=255)
       aaGAGGTGATATTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCt                                                                                                                              <  3:347781/138‑1 (MQ=255)
          agGTGATATTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCttttt                                                                                                                          >  5:440087/1‑139 (MQ=255)
            gTGATATTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTc                                                                                                                         >  2:133698/1‑138 (MQ=255)
             tGATATTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTc                                                                                                                         >  8:75128/1‑137 (MQ=255)
             tGATATTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTc                                                                                                                         >  3:177280/1‑137 (MQ=255)
               atatTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCGGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcagc                                                                                                                     >  8:173281/1‑139 (MQ=255)
               atatTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcag                                                                                                                      >  5:165051/1‑138 (MQ=255)
               atatTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCaag                                                                                                                      >  7:328767/1‑138 (MQ=255)
               atatTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGAGATAGCCTTTTTCcag                                                                                                                      >  8:422397/1‑138 (MQ=255)
                tatTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcagca                                                                                                                    <  1:56631/139‑1 (MQ=255)
                tatTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcagca                                                                                                                    <  1:70056/139‑1 (MQ=255)
                tatTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcagca                                                                                                                    <  2:115301/139‑1 (MQ=255)
                 atTTGATCACCTCTTATAAAATTTCCGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGACCCATAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcag                                                                                                                      >  1:271817/1‑136 (MQ=255)
                 atTTGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcagca                                                                                                                    <  3:2988/138‑1 (MQ=255)
                 atTTGATAACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGATATTTTGTTCCGATCTTAATCCTGAGTGATGTCTGGAAACCTAATGCGTTTCCTGACCGGACTATATAGACTTTATCcag                                                                                                                      >  4:1352/1‑136 (MQ=255)
                    tGATCACCTCTTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGc                                                                                                                >  5:205743/1‑139 (MQ=255)
                           ctctTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGaaa                                                                                                          >  7:61195/1‑138 (MQ=255)
                           ctctTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGaaa                                                                                                          >  5:210752/1‑138 (MQ=255)
                           ctctTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAAc                                                                                                         <  1:132547/139‑1 (MQ=255)
                           ctctTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAAc                                                                                                         <  3:32597/139‑1 (MQ=255)
                           ctctTATAAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTATCCAGCAGAGCCTGAAAc                                                                                                         <  8:120220/139‑1 (MQ=255)
                                ataAAATTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGtt                                                                                                     >  3:172778/1‑138 (MQ=255)
                                     aTTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGCGCCTGAAACAGTTCATcag                                                                                               >  3:325462/1‑137 (MQ=255)
                                     aTTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGa                                                                                                            >  6:231137/1‑126 (MQ=255)
                                     aTTTCAGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGa                                                                                                            <  5:231137/126‑1 (MQ=255)
                                          aGCTAAAATGGCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAgg                                                                                           <  4:188122/138‑1 (MQ=255)
                                                    gCAAGTATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTaaag                                                                                 <  1:133698/138‑2 (MQ=255)
                                                           tttCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTaa                                                                         <  6:205743/139‑1 (MQ=255)
                                                                   ttttGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcagca                                                                                                                    >  1:398427/1‑88 (MQ=255)
                                                                   ttttGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcagca                                                                                                                    <  2:398427/88‑1 (MQ=255)
                                                                   ttttGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACaaaat                                                                 >  1:169621/1‑137 (MQ=255)
                                                                   gtttGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAACCCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGCGCCTGAACCAGTTCATCAGCCGGACGTGTAATGTCAATTAAGACcaaat                                                                 >  5:141053/2‑137 (MQ=255)
                                                                        ctctTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcagca                                                                                                                    <  1:437506/83‑1 (MQ=255)
                                                                        ctctTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcagca                                                                                                                    >  2:437506/1‑83 (MQ=255)
                                                                           ttttGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCt                                                           <  2:169621/137‑1 (MQ=255)
                                                                            tttGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCtgtg                                                        >  2:213245/1‑139 (MQ=255)
                                                                             ttGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCtgtg                                                        <  6:440087/138‑1 (MQ=255)
                                                                                   cGATCGTAATCCTGAGTGATGTCTGGAAAACTAATGCGTTTCCTGACCGGAAGATATAGCCTTTTTACAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTa                                                 <  3:1352/139‑1 (MQ=255)
                                                                                   cGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTa                                                 <  3:85072/139‑1 (MQ=255)
                                                                                    gATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAg                                                <  7:75128/139‑1 (MQ=255)
                                                                                     aTCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAg                                                >  5:418099/1‑138 (MQ=255)
                                                                                     aTCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAg                                                >  7:263162/1‑138 (MQ=255)
                                                                                     aTCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAg                                                >  3:393931/1‑138 (MQ=255)
                                                                                     aTCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAg                                                >  3:209886/1‑138 (MQ=255)
                                                                                     aTCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAAATTGTCTGTGAAGGGTAg                                                >  3:57921/1‑138 (MQ=255)
                                                                                     aTCGTAATCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCC‑TTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCa                                              >  6:226447/1‑139 (MQ=255)
                                                                                           aTCCGGAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACttt                                          <  6:418099/138‑1 (MQ=255)
                                                                                               ggAGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAAc                                      >  2:362490/1‑138 (MQ=255)
                                                                                                 aGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAaca                                                                       >  8:389085/1‑103 (MQ=255)
                                                                                                 aGTGATGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAaca                                                                       <  7:389085/103‑1 (MQ=255)
                                                                                                 aGTGATGTAGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCATGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCAt                                   <  6:22511/139‑1 (MQ=255)
                                                                                                     aTGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGt                               >  1:289087/1‑139 (MQ=255)
                                                                                                      tGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcagca                                                                                                                    <  1:133579/53‑1 (MQ=255)
                                                                                                      tGTCGGGAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCcagca                                                                                                                    >  2:133579/1‑53 (MQ=255)
                                                                                                          gggAAACCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCg                          <  8:61195/139‑1 (MQ=255)
                                                                                                           ggAAACCTAATGCGTTTCCAGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTGTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCg                          >  6:249848/1‑138 (MQ=255)
                                                                                                             aaaCCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCaa                       <  2:289087/139‑1 (MQ=255)
                                                                                                             aaaCCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCaa                       <  2:273414/139‑1 (MQ=255)
                                                                                                             aaaCCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCaa                       <  3:181489/139‑1 (MQ=255)
                                                                                                             aaaCCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCaa                       <  3:286264/139‑1 (MQ=255)
                                                                                                             aaaCCTAATGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCaa                       <  1:229910/139‑1 (MQ=255)
                                                                                                             aaaCCTAATGCGGTTCCTGACCGGACGATATAGCCTTTTTCCAGCCGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCaa                       <  2:271817/139‑1 (MQ=255)
                                                                                                                    aTGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCat                 >  8:282983/1‑138 (MQ=255)
                                                                                                                    aTGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCa                  <  4:209886/137‑1 (MQ=255)
                                                                                                                    aTGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCa                  <  5:114538/137‑1 (MQ=255)
                                                                                                                    aTGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCa                  <  8:263162/137‑1 (MQ=255)
                                                                                                                     tGCGTTTCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCataa               <  1:362490/139‑1 (MQ=255)
                                                                                                                         tttCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCataatact           >  7:154664/1‑137 (MQ=255)
                                                                                                                          ttCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTaaag                                                                                 <  3:78233/68‑2 (MQ=255)
                                                                                                                          ttCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTaaag                                                                                 >  4:78233/1‑67 (MQ=255)
                                                                                                                          ttCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCATAATACTg          >  1:317393/1‑139 (MQ=255)
                                                                                                                           tCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCATAATACTGc         >  1:191401/1‑139 (MQ=255)
                                                                                                                           tCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCATAATACTGc         >  3:359996/1‑139 (MQ=255)
                                                                                                                           tCCTGACCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTGTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCATAATACTGc         >  1:184314/1‑139 (MQ=255)
                                                                                                                                aCCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAaca                                                                       >  1:185528/1‑72 (MQ=255)
                                                                                                                                aCCGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAaca                                                                       <  2:185528/72‑1 (MQ=255)
                                                                                                                                  cGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCATAATACTGCTGAAGGc  <  3:5378/139‑1 (MQ=255)
                                                                                                                                  cGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCATAATACTGCTGAAGGc  <  1:213245/139‑1 (MQ=255)
                                                                                                                                  cGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCATAATACTGCTGAAGGc  <  8:234654/139‑1 (MQ=255)
                                                                                                                                  cGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCATAATACTGCTGAAGGc  <  7:282983/139‑1 (MQ=255)
                                                                                                                                  cGGACGATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCAGCAGGACGTTTAAAGTCAATTAACACAAAATTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTTTTCGCAAAATCATAATACTGCTGAAGGc  <  5:267299/139‑1 (MQ=255)
                                                                                                                                  cGGACGATAGAGCCTATTTCCAGCAGAGCCTGAAACAGTTCATCAGGAGGACGTTTAAAGGCAATTAACACAAAATTTGTCTGTGCAGGGCAGCACTTTAAACCCTGAGTATTCGGAAAATCATAATACTGCTGAAGGc  <  6:98980/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
TAGACTAAAGAGGTGATATTTGATCACCTCTTATAAAATTTCAGCTAAAATTGCAAGAATTTCTTCATTTTGCTCTTTTGTTCCGATCGTAATCCGGAGTGATGTCGGGAAACCTAATGCATTTCCTGACCGGACAATATAGCCTTTTTCCAGCAGAGCCTGAAACAGTTCATCGGAAGGGCGGTTAAAATCAATTAACACAAAGTTTGTCTGTGAAGGGTAGCACTTTAAACCATGAGTCTTCGCAAAATCATAATATTGCTGAAGGC  >  CP006881/2188246‑2188514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑