| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,173,172 | G→A | G192G (GGC→GGT) | U712_10870 ← | Bifunctional protein BirA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,173,172 | 0 | G | A | 98.4% | 184.6 / ‑4.6 | 64 | G192G (GGC→GGT) | U712_10870 | Bifunctional protein BirA |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (30/32); total (31/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01 | |||||||||||
GGAAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAGTTCATCTGGAAAATCATCAGACTGCTGGTTAACGTTAATGCCGATCCCGATGATAACTGACCGTACGCGGTCTTCCTCGGCCTGCATTTCGGTTAAGATGCCGACTGTTTTTTTTCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCG > CP006881/2173037‑2173305 | agcaGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCg > 7:137412/4‑139 (MQ=255) caGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGAc < 4:262514/138‑1 (MQ=255) caGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGAc < 6:73119/138‑1 (MQ=255) caGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGAc < 3:322248/138‑1 (MQ=255) tCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGttaat > 4:25163/1‑139 (MQ=255) tGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATGACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAat < 8:256821/139‑1 (MQ=255) gTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAata > 2:112803/1‑139 (MQ=255) caattCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCAttt > 1:302026/4‑138 (MQ=255) cATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGcc < 7:42391/138‑1 (MQ=255) aTCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCCGCCGGCCTTTCCGATAGGATACCGCCTGTGTTTTTTCCGTTAAGCAAAATATCATTTGGCCag > 1:283486/1‑138 (MQ=255) cTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCAttt > 2:312852/1‑139 (MQ=255) gAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGCCTGTGTTTTTTCCGTTAATCAAAATTTCATTTGGCCATTTaa > 7:311902/1‑138 (MQ=255) aaaaTCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAata < 6:403864/139‑1 (MQ=255) atcattAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATcc < 2:4726/139‑1 (MQ=255) attaggctgctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGt < 8:317489/132‑1 (MQ=255) ggctgctgGTTAACGTTATTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATTCCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGa < 6:145175/137‑1 (MQ=255) ctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGc < 8:269824/115‑1 (MQ=255) ctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGc > 7:269824/1‑115 (MQ=255) ctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCct > 2:24451/1‑139 (MQ=255) ctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCct > 2:20125/1‑139 (MQ=255) ctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCct < 1:228886/139‑1 (MQ=255) ctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATAATTTGGCCATTTAATATCCTTTTGGATGCct > 7:148667/1‑139 (MQ=255) gTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCctgct < 6:81155/139‑1 (MQ=255) aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCct > 8:16554/1‑133 (MQ=255) aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCct < 7:16554/133‑1 (MQ=255) aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc < 7:205954/138‑1 (MQ=255) aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc < 1:112803/138‑1 (MQ=255) aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc < 8:137412/138‑1 (MQ=255) aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc < 4:217751/138‑1 (MQ=255) aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc < 3:15395/138‑1 (MQ=255) aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc < 2:36398/138‑1 (MQ=255) aCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGcct < 8:135781/139‑2 (MQ=255) aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGGATTTGCGTTATGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCAGTTGGCCATATAATATCCGTTTGGTTTCCTGCTGCctctgcg > 4:262231/1‑135 (MQ=255) aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAatat > 5:239164/1‑112 (MQ=255) aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAatat < 6:239164/112‑1 (MQ=255) aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTa < 1:312852/139‑1 (MQ=255) aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTa > 5:207600/1‑139 (MQ=255) aTCCCGATGATAACTGACCGTACGCGGTCTTCCTCGGCCTGCATTTCGGTTAAGATGCCGACTGTTTTTTTTCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGATTGGATTCCCt > 7:255523/1‑139 (MQ=255) aTCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGCCTGTTTTTTTTCCGTTAATCAAAATATCATGTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCGATTCCCt > 2:152074/1‑139 (MQ=255) aTCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCt > 8:309226/1‑139 (MQ=255) aTCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCt > 2:51337/1‑139 (MQ=255) tgatCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCacaac < 2:302026/139‑1 (MQ=255) tgatCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCacaac < 2:260607/139‑1 (MQ=255) aCTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGTTACCGGCTGGTTTTTTTACGTTAATCAAAATATCATTTGGCCATTTTATATCCGTTTGGATGCCTGCTGCCTCGTCTTTTCCCTGCCCCACAGCTa > 4:9052/1‑139 (MQ=255) aCTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACAGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGTCCATTTAATATCCGTATTGATGCATGCTGACTATTCTATTACCTGCACAACAGCTa > 8:19510/1‑139 (MQ=255) aacGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTg > 8:410592/3‑138 (MQ=255) aCGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTcc < 6:390717/116‑1 (MQ=255) aCGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCAATTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTcc > 5:390717/1‑116 (MQ=255) cgGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc > 4:116365/1‑101 (MQ=255) cgGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc < 3:116365/101‑1 (MQ=255) cgGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcac > 5:208091/1‑119 (MQ=255) cgGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcac < 6:208091/119‑1 (MQ=255) cgGTCTTCTTCAGCCTGCATTTCCGGTTGGATACCGCCTGGTTTTTTTCCGGTAATCAAAATATCATTTGGCCATTTTATATGCGTTTGGATGCCTGCTGCGTCGTGTATTCCCTGCACAACAGCTACTTCAGCAAg > 7:222228/1‑137 (MQ=255) gTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGTATGCCtgctgc > 6:217721/1‑99 (MQ=255) gTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc < 5:217721/99‑1 (MQ=255) gCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCg < 5:148001/139‑1 (MQ=255) ccTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcac > 2:49798/1‑106 (MQ=255) ccTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcac < 1:49798/106‑1 (MQ=255) ccTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCgg > 5:279196/1‑139 (MQ=255) cATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGGGtt > 5:69217/1‑139 (MQ=255) tccgTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGttttt < 3:25163/135‑1 (MQ=255) aggATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGgt < 2:126118/124‑1 (MQ=255) aggATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGgt > 1:126118/3‑126 (MQ=255) ggATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCg < 8:170068/137‑1 (MQ=255) | GGAAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAGTTCATCTGGAAAATCATCAGACTGCTGGTTAACGTTAATGCCGATCCCGATGATAACTGACCGTACGCGGTCTTCCTCGGCCTGCATTTCGGTTAAGATGCCGACTGTTTTTTTTCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCG > CP006881/2173037‑2173305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |