Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,188,849 T→A S170S (TCA→TCT)  U712_10965 ← Histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,188,8490TA97.2% 218.3 / NA 72S170S (TCA→TCT) U712_10965Histidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (37/33);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTAATAACGGCACAGTTTCCGGGTAATCCTCAGCTGTTACATATTCATAATATGCTTCGTCAAGAACAACGAGGACATGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCCCCTTCTGATGTATAAGTGCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCC  >  CP006881/2188729‑2188983
                                                                                                                        |                                                                                                                                      
gcttAAGAGCGGCACGGTTTCCGGGTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGt                                                                                                                       <  4:227736/136‑1 (MQ=255)
  ttAAGAGCGGCTCGGTTTCCGGGTTATCCTCTGCTGTTACAGAGTCATAATATGCTTCGTCTAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCATGCAGATGTATAAGTGCCAGTCgg                                                                                                                    >  4:58718/1‑139 (MQ=255)
    aagagcGGCACGGTTTCCGGGTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCgga                                                                                                                   <  5:130095/133‑2 (MQ=255)
          ggCACGGTTTCCGGGTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCg                                                                                                            <  4:409804/139‑1 (MQ=255)
           gCACGGTTTCCGGGTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCgg                                                                                                           <  1:442379/139‑1 (MQ=255)
            cacgGTTTCCGGGTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCggg                                                                                                          >  5:381072/1‑138 (MQ=255)
            cacgGTTTCCGGGTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGAATAAGTGCCAGTCGGATTATTCggg                                                                                                          >  4:117578/1‑138 (MQ=255)
                gTTTCCGGGTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGc                                                                                                      <  6:381072/139‑1 (MQ=255)
                       ggTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCa                                                                                               <  3:12827/139‑1 (MQ=255)
                           aTCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAAc                                                                                            >  5:181330/1‑138 (MQ=255)
                           aTCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAAc                                                                                            >  1:403055/1‑138 (MQ=255)
                                 tgCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTtgt                                                                                     >  7:80855/2‑139 (MQ=255)
                                 tgCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTtgt                                                                                     >  7:413850/2‑139 (MQ=255)
                                      ttACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTg                                                                                >  6:431656/1‑139 (MQ=255)
                                        aCATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGtt                                                                              >  7:243825/1‑139 (MQ=255)
                                        aCATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGtt                                                                              >  2:152924/1‑139 (MQ=255)
                                           tatTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGATCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCAAAACGACTTGTGTCTGTTCGt                                                                           >  8:290499/1‑139 (MQ=255)
                                             ttCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTc                                                                          >  4:49737/1‑138 (MQ=255)
                                              tCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTcga                                                                        >  8:242429/1‑137 (MQ=255)
                                              tCATAATATGCTTCGCCAAGAACGCCAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAACCGACTT‑TGTCTGTTCGTCGAt                                                                       >  6:391956/1‑139 (MQ=255)
                                               cATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATACGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTcga                                                                        <  1:135464/138‑3 (MQ=255)
                                               cATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTcga                                                                        <  1:96761/138‑3 (MQ=255)
                                               cATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTcga                                                                        <  8:428205/138‑3 (MQ=255)
                                                ataataTGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAtg                                                                      >  8:331956/1‑138 (MQ=255)
                                                ataataTGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAtg                                                                      >  3:391647/1‑138 (MQ=255)
                                                ataataTGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAtg                                                                      >  3:379745/1‑138 (MQ=255)
                                                ataataTGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAt                                                                       <  3:49737/138‑1 (MQ=255)
                                                ataataTGCTGCGTCAAGAACGACAAGCACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCTGATGTATAAGTGCCAGTCGTATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAt                                                                       <  8:33739/138‑1 (MQ=255)
                                                   atatGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGc                                                                    <  3:66739/138‑1 (MQ=255)
                                                   atatGCTTCGACATGAACGACAAGGACACGAGAAGTAACGCGCTCCAGATATGCGCGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGGTGGATATCCAAACGACTTGTGTCTGTTCGTCGATGGc                                                                    <  4:305509/138‑1 (MQ=255)
                                                      tGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTc                                                                 >  8:182176/1‑138 (MQ=255)
                                                       gCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTct                                                                >  5:367440/1‑137 (MQ=255)
                                                        cTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTcga                                                                        >  6:16343/1‑127 (MQ=255)
                                                        cTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTctaa                                                              >  7:20380/1‑136 (MQ=255)
                                                        cTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGAATATCCAAACGACTTGTGTCTGTTCGTcga                                                                        <  5:16343/129‑3 (MQ=255)
                                                          tCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTctaaca                                                            >  7:201582/1‑134 (MQ=255)
                                                          tCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTctaaca                                                            >  5:222438/1‑134 (MQ=255)
                                                          tCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTctaaca                                                            >  4:304399/1‑134 (MQ=255)
                                                           cGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACAt                                                           >  6:32496/1‑139 (MQ=255)
                                                            ttCAACAACTACAAGGACACTAGAAGGAACCCGCTCCTTAAATGCGATTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACAt                                                           <  4:239469/137‑1 (MQ=255)
                                                            gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACAt                                                           <  3:61087/138‑1 (MQ=255)
                                                            gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATg                                                          <  5:121740/139‑1 (MQ=255)
                                                            gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATg                                                          <  8:360745/139‑1 (MQ=255)
                                                            gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTAGAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTACCATg                                                          >  8:82621/1‑139 (MQ=255)
                                                               atcaacgacAAGGACACGAGAAGCAAGGCGTTCCACAAATGCGAGTAATTCCCCTTCTTTTGTATAAGTGCCTGTCGGATTATTCGGGGTGCATATCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATgg                                                         <  1:286635/134‑1 (MQ=255)
                                                                 gAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTc                                                     >  8:367425/1‑139 (MQ=255)
                                                                  aacgacAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTc                                                     >  4:224598/1‑138 (MQ=255)
                                                                     gacaaGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTcaa                                                 <  5:431656/135‑1 (MQ=255)
                                                                       caaGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAAt                                                >  5:145411/3‑138 (MQ=255)
                                                                       caaGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAAt                                                >  3:239469/3‑138 (MQ=255)
                                                                            acacGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCAtgt                                           >  2:38831/1‑138 (MQ=255)
                                                                             cacgagaAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGa                                         <  8:413850/136‑1 (MQ=255)
                                                                                   aaGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTc                                   <  1:152924/139‑1 (MQ=255)
                                                                                                cAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTg                        <  4:436514/137‑1 (MQ=255)
                                                                                                      tGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCAGGTTAGCCGGCGGGACGAATGGCAATTTc                 >  1:432680/1‑138 (MQ=255)
                                                                                                      tGCGAGTAATTATCCTTCAGATGTATCAGTGCCTGGCGGATTATTCGGGCGGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCa                <  3:58718/139‑1 (MQ=255)
                                                                                                        cGAGTATTTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg              <  3:363669/139‑1 (MQ=255)
                                                                                                        cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCgg                                                                                                           >  3:316417/1‑46 (MQ=255)
                                                                                                        cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCgg                                                                                                           <  4:316417/46‑1 (MQ=37)
                                                                                                        cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg              >  4:192326/1‑139 (MQ=255)
                                                                                                        cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg              >  8:176394/1‑139 (MQ=255)
                                                                                                        cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg              <  3:304399/139‑1 (MQ=255)
                                                                                                        cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg              >  8:334439/1‑139 (MQ=255)
                                                                                                        cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg              <  4:391647/139‑1 (MQ=255)
                                                                                                        cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg              <  6:181330/139‑1 (MQ=255)
                                                                                                        cGAGTAATTCGCCTTCACAGGTATACGTGCCAGGCGGATAAGTCGGGCGGCATAGCCAAACGACTTGTGTCTGTTCGTCGATCGCTGCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg              <  6:141053/139‑1 (MQ=255)
                                                                                                              aTTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTc        <  7:242429/139‑1 (MQ=255)
                                                                                                                 ctccTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAg      <  3:117578/136‑1 (MQ=255)
                                                                                                                 ctccTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAg      <  4:379745/136‑1 (MQ=255)
                                                                                                                  tccTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAgca    <  6:145411/138‑2 (MQ=255)
                                                                                                                    cTTCAGATGTTTAAGTGCCAGTCGGATTTTTCGGGCTGCATATCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGGCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAgca    <  6:367440/137‑2 (MQ=255)
                                                                                                                    cTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCAcc  >  3:28152/1‑139 (MQ=255)
                                                                                                                        |                                                                                                                                      
GTTTAATAACGGCACAGTTTCCGGGTAATCCTCAGCTGTTACATATTCATAATATGCTTCGTCAAGAACAACGAGGACATGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCCCCTTCTGATGTATAAGTGCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCC  >  CP006881/2188729‑2188983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑