Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,188,870 G→A N163N (AAC→AAT)  U712_10965 ← Histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,188,8700GA97.6% 253.1 / ‑3.1 84N163N (AAC→AAT) U712_10965Histidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (42/40);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

GGCACAGTTTCCGGGTAATCCTCAGCTGTTACATATTCATAATATGCTTCGTCAAGAACAACGAGGACATGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCCCCTTCTGATGTATAAGTGCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATGACCGCATTATGT  >  CP006881/2188739‑2189002
                                                                                                                                   |                                                                                                                                    
ggCACGGTTTCCGGGTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCg                                                                                                                               <  4:409804/139‑1 (MQ=255)
 gCACGGTTTCCGGGTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCgg                                                                                                                              <  1:442379/139‑1 (MQ=255)
  cacgGTTTCCGGGTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCggg                                                                                                                             >  5:381072/1‑138 (MQ=255)
  cacgGTTTCCGGGTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGAATAAGTGCCAGTCGGATTATTCggg                                                                                                                             >  4:117578/1‑138 (MQ=255)
      gTTTCCGGGTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGc                                                                                                                         <  6:381072/139‑1 (MQ=255)
             ggTAATCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCa                                                                                                                  <  3:12827/139‑1 (MQ=255)
                 aTCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAAc                                                                                                               >  1:403055/1‑138 (MQ=255)
                 aTCCTCTGCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAAc                                                                                                               >  5:181330/1‑138 (MQ=255)
                       tgCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTtgt                                                                                                        >  7:413850/2‑139 (MQ=255)
                       tgCTGTTACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTtgt                                                                                                        >  7:80855/2‑139 (MQ=255)
                            ttACATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTg                                                                                                   >  6:431656/1‑139 (MQ=255)
                              aCATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGtt                                                                                                 >  2:152924/1‑139 (MQ=255)
                              aCATATTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGtt                                                                                                 >  7:243825/1‑139 (MQ=255)
                                 tatTCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGATCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCAAAACGACTTGTGTCTGTTCGt                                                                                              >  8:290499/1‑139 (MQ=255)
                                   ttCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTc                                                                                             >  4:49737/1‑138 (MQ=255)
                                    tCATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTcga                                                                                           >  8:242429/1‑137 (MQ=255)
                                    tCATAATATGCTTCGCCAAGAACGCCAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAACCGACTT‑TGTCTGTTCGTCGAt                                                                                          >  6:391956/1‑139 (MQ=255)
                                     cATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATACGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTcga                                                                                           <  1:135464/138‑3 (MQ=255)
                                     cATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTcga                                                                                           <  8:428205/138‑3 (MQ=255)
                                     cATAATATGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTcga                                                                                           <  1:96761/138‑3 (MQ=255)
                                      ataataTGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAtg                                                                                         >  3:379745/1‑138 (MQ=255)
                                      ataataTGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAtg                                                                                         >  3:391647/1‑138 (MQ=255)
                                      ataataTGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAtg                                                                                         >  8:331956/1‑138 (MQ=255)
                                      ataataTGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAt                                                                                          <  3:49737/138‑1 (MQ=255)
                                      ataataTGCTGCGTCAAGAACGACAAGCACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCTGATGTATAAGTGCCAGTCGTATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAt                                                                                          <  8:33739/138‑1 (MQ=255)
                                         atatGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGc                                                                                       <  3:66739/138‑1 (MQ=255)
                                         atatGCTTCGACATGAACGACAAGGACACGAGAAGTAACGCGCTCCAGATATGCGCGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGGTGGATATCCAAACGACTTGTGTCTGTTCGTCGATGGc                                                                                       <  4:305509/138‑1 (MQ=255)
                                            tGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTc                                                                                    >  8:182176/1‑138 (MQ=255)
                                             gCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTct                                                                                   >  5:367440/1‑137 (MQ=255)
                                              cTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTcga                                                                                           >  6:16343/1‑127 (MQ=255)
                                              cTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTctaa                                                                                 >  7:20380/1‑136 (MQ=255)
                                              cTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGAATATCCAAACGACTTGTGTCTGTTCGTcga                                                                                           <  5:16343/129‑3 (MQ=255)
                                                tCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTctaaca                                                                               >  7:201582/1‑134 (MQ=255)
                                                tCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTctaaca                                                                               >  4:304399/1‑134 (MQ=255)
                                                tCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTctaaca                                                                               >  5:222438/1‑134 (MQ=255)
                                                 cGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACAt                                                                              >  6:32496/1‑139 (MQ=255)
                                                  ttCAACAACTACAAGGACACTAGAAGGAACCCGCTCCTTAAATGCGATTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACAt                                                                              <  4:239469/137‑1 (MQ=255)
                                                  gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACAt                                                                              <  3:61087/138‑1 (MQ=255)
                                                  gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATg                                                                             <  8:360745/139‑1 (MQ=255)
                                                  gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATg                                                                             <  5:121740/139‑1 (MQ=255)
                                                  gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTAGAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTACCATg                                                                             >  8:82621/1‑139 (MQ=255)
                                                     atcaacgacAAGGACACGAGAAGCAAGGCGTTCCACAAATGCGAGTAATTCCCCTTCTTTTGTATAAGTGCCTGTCGGATTATTCGGGGTGCATATCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATgg                                                                            <  1:286635/134‑1 (MQ=255)
                                                       gAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTc                                                                        >  8:367425/1‑139 (MQ=255)
                                                        aacgacAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTc                                                                        >  4:224598/1‑138 (MQ=255)
                                                           gacaaGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTcaa                                                                    <  5:431656/135‑1 (MQ=255)
                                                             caaGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAAt                                                                   >  5:145411/3‑138 (MQ=255)
                                                             caaGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAAt                                                                   >  3:239469/3‑138 (MQ=255)
                                                                  acacGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCAtgt                                                              >  2:38831/1‑138 (MQ=255)
                                                                   cacgagaAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGa                                                            <  8:413850/136‑1 (MQ=255)
                                                                         aaGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTc                                                      <  1:152924/139‑1 (MQ=255)
                                                                                      cAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTg                                           <  4:436514/137‑1 (MQ=255)
                                                                                            tGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCAGGTTAGCCGGCGGGACGAATGGCAATTTc                                    >  1:432680/1‑138 (MQ=255)
                                                                                            tGCGAGTAATTATCCTTCAGATGTATCAGTGCCTGGCGGATTATTCGGGCGGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCa                                   <  3:58718/139‑1 (MQ=255)
                                                                                              cGAGTATTTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                 <  3:363669/139‑1 (MQ=255)
                                                                                              cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCgg                                                                                                                              >  3:316417/1‑46 (MQ=255)
                                                                                              cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCgg                                                                                                                              <  4:316417/46‑1 (MQ=37)
                                                                                              cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                 <  4:391647/139‑1 (MQ=255)
                                                                                              cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                 >  8:334439/1‑139 (MQ=255)
                                                                                              cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                 >  4:192326/1‑139 (MQ=255)
                                                                                              cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                 >  8:176394/1‑139 (MQ=255)
                                                                                              cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                 <  3:304399/139‑1 (MQ=255)
                                                                                              cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                 <  6:181330/139‑1 (MQ=255)
                                                                                              cGAGTAATTCGCCTTCACAGGTATACGTGCCAGGCGGATAAGTCGGGCGGCATAGCCAAACGACTTGTGTCTGTTCGTCGATCGCTGCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                 <  6:141053/139‑1 (MQ=255)
                                                                                                    aTTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTc                           <  7:242429/139‑1 (MQ=255)
                                                                                                       ctccTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAg                         <  3:117578/136‑1 (MQ=255)
                                                                                                       ctccTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAg                         <  4:379745/136‑1 (MQ=255)
                                                                                                        tccTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAgca                       <  6:145411/138‑2 (MQ=255)
                                                                                                          cTTCAGATGTTTAAGTGCCAGTCGGATTTTTCGGGCTGCATATCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGGCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAgca                       <  6:367440/137‑2 (MQ=255)
                                                                                                          cTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCAcc                     >  3:28152/1‑139 (MQ=255)
                                                                                                                 tGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAAta              <  3:192326/139‑2 (MQ=255)
                                                                                                                     taAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCg          >  6:213581/1‑139 (MQ=255)
                                                                                                                        gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt       <  4:28152/139‑1 (MQ=255)
                                                                                                                        gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt       <  3:224598/139‑1 (MQ=255)
                                                                                                                        gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt       <  8:201582/139‑1 (MQ=255)
                                                                                                                        gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt       <  6:222438/139‑1 (MQ=255)
                                                                                                                        gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt       <  5:32496/139‑1 (MQ=255)
                                                                                                                          gCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATg              <  3:155194/130‑1 (MQ=255)
                                                                                                                          gCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATg              >  4:155194/1‑130 (MQ=255)
                                                                                                                            cAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGATGTGCAAATTCACGTACTTCAGCACCTTCAATAACCGCATTaag   >  3:88293/1‑137 (MQ=255)
                                                                                                                             aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCa                                   >  5:104756/1‑106 (MQ=255)
                                                                                                                             aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCa                                   <  6:104756/106‑1 (MQ=255)
                                                                                                                             aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGt  >  8:305377/1‑139 (MQ=255)
                                                                                                                             aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGt  >  4:253402/1‑139 (MQ=255)
                                                                                                                             aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGt  >  5:401940/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                    
GGCACAGTTTCCGGGTAATCCTCAGCTGTTACATATTCATAATATGCTTCGTCAAGAACAACGAGGACATGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCCCCTTCTGATGTATAAGTGCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATGACCGCATTATGT  >  CP006881/2188739‑2189002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑