Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,188,912 A→G I149I (ATT→ATC)  U712_10965 ← Histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,188,9120AG97.7% 259.3 / ‑3.3 85I149I (ATT→ATC) U712_10965Histidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (38/45);  total (39/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01

TCATAATATGCTTCGTCAAGAACAACGAGGACATGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCCCCTTCTGATGTATAAGTGCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATGACCGCATTATGTTTATATTGCGGAAAAGTCGGGGCAGCAGTTATCGTGTTAGTTTTA  >  CP006881/2188775‑2189047
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tCATAATATGCTTCGCCAAGAACGCCAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAACCGACTT‑TGTCTGTTCGTCGAt                                                                                                                                       >  6:391956/1‑139 (MQ=255)
  ataataTGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAtg                                                                                                                                      >  3:379745/1‑138 (MQ=255)
  ataataTGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAtg                                                                                                                                      >  3:391647/1‑138 (MQ=255)
  ataataTGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAtg                                                                                                                                      >  8:331956/1‑138 (MQ=255)
  ataataTGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAt                                                                                                                                       <  3:49737/138‑1 (MQ=255)
  ataataTGCTGCGTCAAGAACGACAAGCACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCTGATGTATAAGTGCCAGTCGTATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGAt                                                                                                                                       <  8:33739/138‑1 (MQ=255)
     atatGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGc                                                                                                                                    <  3:66739/138‑1 (MQ=255)
     atatGCTTCGACATGAACGACAAGGACACGAGAAGTAACGCGCTCCAGATATGCGCGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGGTGGATATCCAAACGACTTGTGTCTGTTCGTCGATGGc                                                                                                                                    <  4:305509/138‑1 (MQ=255)
        tGCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTc                                                                                                                                 >  8:182176/1‑138 (MQ=255)
         gCTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTct                                                                                                                                >  5:367440/1‑137 (MQ=255)
          cTTCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTctaa                                                                                                                              >  7:20380/1‑136 (MQ=255)
            tCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTctaaca                                                                                                                            >  5:222438/1‑134 (MQ=255)
            tCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTctaaca                                                                                                                            >  4:304399/1‑134 (MQ=255)
            tCGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTctaaca                                                                                                                            >  7:201582/1‑134 (MQ=255)
             cGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACAt                                                                                                                           >  6:32496/1‑139 (MQ=255)
              ttCAACAACTACAAGGACACTAGAAGGAACCCGCTCCTTAAATGCGATTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACAt                                                                                                                           <  4:239469/137‑1 (MQ=255)
              gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACAt                                                                                                                           <  3:61087/138‑1 (MQ=255)
              gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATg                                                                                                                          <  5:121740/139‑1 (MQ=255)
              gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATg                                                                                                                          <  8:360745/139‑1 (MQ=255)
              gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTAGAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTACCATg                                                                                                                          >  8:82621/1‑139 (MQ=255)
                 atcaacgacAAGGACACGAGAAGCAAGGCGTTCCACAAATGCGAGTAATTCCCCTTCTTTTGTATAAGTGCCTGTCGGATTATTCGGGGTGCATATCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATgg                                                                                                                         <  1:286635/134‑1 (MQ=255)
                   gAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTc                                                                                                                     >  8:367425/1‑139 (MQ=255)
                    aacgacAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTc                                                                                                                     >  4:224598/1‑138 (MQ=255)
                       gacaaGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTcaa                                                                                                                 <  5:431656/135‑1 (MQ=255)
                         caaGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAAt                                                                                                                >  5:145411/3‑138 (MQ=255)
                         caaGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAAt                                                                                                                >  3:239469/3‑138 (MQ=255)
                              acacGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCAtgt                                                                                                           >  2:38831/1‑138 (MQ=255)
                               cacgagaAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGa                                                                                                         <  8:413850/136‑1 (MQ=255)
                                     aaGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTc                                                                                                   <  1:152924/139‑1 (MQ=255)
                                                  cAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTg                                                                                        <  4:436514/137‑1 (MQ=255)
                                                        tGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCAGGTTAGCCGGCGGGACGAATGGCAATTTc                                                                                 >  1:432680/1‑138 (MQ=255)
                                                        tGCGAGTAATTATCCTTCAGATGTATCAGTGCCTGGCGGATTATTCGGGCGGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCa                                                                                <  3:58718/139‑1 (MQ=255)
                                                          cGAGTATTTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                              <  3:363669/139‑1 (MQ=255)
                                                          cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                              <  6:181330/139‑1 (MQ=255)
                                                          cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                              >  8:334439/1‑139 (MQ=255)
                                                          cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                              <  3:304399/139‑1 (MQ=255)
                                                          cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                              >  8:176394/1‑139 (MQ=255)
                                                          cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                              <  4:391647/139‑1 (MQ=255)
                                                          cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                              >  4:192326/1‑139 (MQ=255)
                                                          cGAGTAATTCGCCTTCACAGGTATACGTGCCAGGCGGATAAGTCGGGCGGCATAGCCAAACGACTTGTGTCTGTTCGTCGATCGCTGCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                              <  6:141053/139‑1 (MQ=255)
                                                                aTTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTc                                                                        <  7:242429/139‑1 (MQ=255)
                                                                   ctccTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAg                                                                      <  4:379745/136‑1 (MQ=255)
                                                                   ctccTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAg                                                                      <  3:117578/136‑1 (MQ=255)
                                                                    tccTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAgca                                                                    <  6:145411/138‑2 (MQ=255)
                                                                      cTTCAGATGTTTAAGTGCCAGTCGGATTTTTCGGGCTGCATATCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGGCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAgca                                                                    <  6:367440/137‑2 (MQ=255)
                                                                      cTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCAcc                                                                  >  3:28152/1‑139 (MQ=255)
                                                                             tGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAAta                                                           <  3:192326/139‑2 (MQ=255)
                                                                                 taAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCg                                                       >  6:213581/1‑139 (MQ=255)
                                                                                    gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                    <  3:224598/139‑1 (MQ=255)
                                                                                    gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                    <  8:201582/139‑1 (MQ=255)
                                                                                    gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                    <  4:28152/139‑1 (MQ=255)
                                                                                    gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                    <  6:222438/139‑1 (MQ=255)
                                                                                    gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                    <  5:32496/139‑1 (MQ=255)
                                                                                      gCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATg                                                           >  4:155194/1‑130 (MQ=255)
                                                                                      gCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATg                                                           <  3:155194/130‑1 (MQ=255)
                                                                                        cAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGATGTGCAAATTCACGTACTTCAGCACCTTCAATAACCGCATTaag                                                >  3:88293/1‑137 (MQ=255)
                                                                                         aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCa                                                                                <  6:104756/106‑1 (MQ=255)
                                                                                         aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCa                                                                                >  5:104756/1‑106 (MQ=255)
                                                                                         aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGt                                               >  4:253402/1‑139 (MQ=255)
                                                                                         aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGt                                               >  5:401940/1‑139 (MQ=255)
                                                                                         aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGt                                               >  8:305377/1‑139 (MQ=255)
                                                                                              gattATTCGGGATGCATATCCAAACGACTTTTGTCTGTTAGTCGATTGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTAGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTtata                                          <  2:432680/137‑1 (MQ=255)
                                                                                               attATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAAGCATGTGAGCCGTCGGGACGAAGTGCAAGTTAACGTACTTCAGCACCTTCAATAACCGCATTATGTTTatat                                         >  7:260716/2‑139 (MQ=255)
                                                                                                        gcTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGcg                                                                                                                       <  3:420767/51‑2 (MQ=255)
                                                                                                        gcTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGcg                                                                                                                       >  4:420767/2‑51 (MQ=255)
                                                                                                        gcTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCa                                                     <  3:355777/117‑1 (MQ=255)
                                                                                                        gcTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCa                                                     >  4:355777/2‑118 (MQ=255)
                                                                                                            cATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGt                              <  3:253402/137‑1 (MQ=255)
                                                                                                             atatCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCATCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTggg                           >  4:331805/1‑139 (MQ=255)
                                                                                                                tCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgc                        <  7:176394/139‑1 (MQ=255)
                                                                                                                tCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgc                        <  7:182176/139‑1 (MQ=255)
                                                                                                                gccAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgc                        <  3:164438/138‑1 (MQ=255)
                                                                                                                  cAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATCGCGGCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCTTTATGTTTATATTGCGGAAAAGTGGGGgcag                      <  7:33512/139‑1 (MQ=255)
                                                                                                                    aaCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                    >  3:100699/1‑107 (MQ=255)
                                                                                                                    aaCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                    <  4:100699/107‑1 (MQ=255)
                                                                                                                    aaCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGt                              >  2:390101/1‑129 (MQ=255)
                                                                                                                    aaCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGt                              <  1:390101/129‑1 (MQ=255)
                                                                                                                    aaCGACTTGTGTCTGTGCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgcagca                    <  8:20380/139‑1 (MQ=255)
                                                                                                                     aCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGcagcag                   <  2:403055/139‑1 (MQ=255)
                                                                                                                      cGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCAAGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGcagcag                   >  2:100956/1‑138 (MQ=255)
                                                                                                                         cTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGtaac               <  5:213581/139‑4 (MQ=255)
                                                                                                                            gtgtCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGAAATTTAACGTACATCCTCACCTTCAAATACCGCATTATGTTTATATTTCGGAAAAGTTGGGGCAGCAGtacacgc            >  4:164438/1‑133 (MQ=255)
                                                                                                                                 tGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTAAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGtttg       >  6:393617/1‑137 (MQ=255)
                                                                                                                                   ttCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCCCGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGgttgtt     >  7:412948/1‑139 (MQ=255)
                                                                                                                                      gTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTa  <  7:290499/139‑1 (MQ=255)
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TCATAATATGCTTCGTCAAGAACAACGAGGACATGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCCCCTTCTGATGTATAAGTGCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATGACCGCATTATGTTTATATTGCGGAAAAGTCGGGGCAGCAGTTATCGTGTTAGTTTTA  >  CP006881/2188775‑2189047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑