Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,188,923 G→A L146L (CTT→TTA)  U712_10965 ← Histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,188,9230GA97.3% 216.7 / ‑2.7 75L146L (CTT→TTA) U712_10965Histidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (30/42);  total (32/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

CGTCAAGAACAACGAGGACATGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCCCCTTCTGATGTATAAGTGCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATGACCGCATTATGTTTATATTGCGGAAAAGTCGGGGCAGCAGTTATCGTGTTAGTTTTATCATTT  >  CP006881/2188788‑2189053
                                                                                                                                       |                                                                                                                                  
cGTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACAt                                                                                                                                 >  6:32496/1‑139 (MQ=255)
 ttCAACAACTACAAGGACACTAGAAGGAACCCGCTCCTTAAATGCGATTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACAt                                                                                                                                 <  4:239469/137‑1 (MQ=255)
 gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACAt                                                                                                                                 <  3:61087/138‑1 (MQ=255)
 gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATg                                                                                                                                <  8:360745/139‑1 (MQ=255)
 gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATg                                                                                                                                <  5:121740/139‑1 (MQ=255)
 gTCAAGAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTAGAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTACCATg                                                                                                                                >  8:82621/1‑139 (MQ=255)
    atcaacgacAAGGACACGAGAAGCAAGGCGTTCCACAAATGCGAGTAATTCCCCTTCTTTTGTATAAGTGCCTGTCGGATTATTCGGGGTGCATATCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATgg                                                                                                                               <  1:286635/134‑1 (MQ=255)
      gAACGACAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTc                                                                                                                           >  8:367425/1‑139 (MQ=255)
       aacgacAAGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTc                                                                                                                           >  4:224598/1‑138 (MQ=255)
          gacaaGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTcaa                                                                                                                       <  5:431656/135‑1 (MQ=255)
            caaGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAAt                                                                                                                      >  5:145411/3‑138 (MQ=255)
            caaGGACACGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAAt                                                                                                                      >  3:239469/3‑138 (MQ=255)
                 acacGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCAtgt                                                                                                                 >  2:38831/1‑138 (MQ=255)
                  cacgagaAGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGa                                                                                                               <  8:413850/136‑1 (MQ=255)
                        aaGGAACGCGCTCCAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTc                                                                                                         <  1:152924/139‑1 (MQ=255)
                                     cAGAAATGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTg                                                                                              <  4:436514/137‑1 (MQ=255)
                                           tGCGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCAGGTTAGCCGGCGGGACGAATGGCAATTTc                                                                                       >  1:432680/1‑138 (MQ=255)
                                           tGCGAGTAATTATCCTTCAGATGTATCAGTGCCTGGCGGATTATTCGGGCGGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCa                                                                                      <  3:58718/139‑1 (MQ=255)
                                             cGAGTATTTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                                    <  3:363669/139‑1 (MQ=255)
                                             cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                                    >  8:176394/1‑139 (MQ=255)
                                             cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                                    <  3:304399/139‑1 (MQ=255)
                                             cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                                    <  6:181330/139‑1 (MQ=255)
                                             cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                                    <  4:391647/139‑1 (MQ=255)
                                             cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                                    >  4:192326/1‑139 (MQ=255)
                                             cGAGTAATTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                                    >  8:334439/1‑139 (MQ=255)
                                             cGAGTAATTCGCCTTCACAGGTATACGTGCCAGGCGGATAAGTCGGGCGGCATAGCCAAACGACTTGTGTCTGTTCGTCGATCGCTGCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                                    <  6:141053/139‑1 (MQ=255)
                                                   aTTCTCCTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTc                                                                              <  7:242429/139‑1 (MQ=255)
                                                      ctccTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAg                                                                            <  3:117578/136‑1 (MQ=255)
                                                      ctccTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAg                                                                            <  4:379745/136‑1 (MQ=255)
                                                       tccTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAgca                                                                          <  6:145411/138‑2 (MQ=255)
                                                         cTTCAGATGTTTAAGTGCCAGTCGGATTTTTCGGGCTGCATATCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGGCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAgca                                                                          <  6:367440/137‑2 (MQ=255)
                                                         cTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCAcc                                                                        >  3:28152/1‑139 (MQ=255)
                                                                tGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAAta                                                                 <  3:192326/139‑2 (MQ=255)
                                                                    taAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCg                                                             >  6:213581/1‑139 (MQ=255)
                                                                       gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                          <  5:32496/139‑1 (MQ=255)
                                                                       gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                          <  8:201582/139‑1 (MQ=255)
                                                                       gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                          <  4:28152/139‑1 (MQ=255)
                                                                       gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                          <  6:222438/139‑1 (MQ=255)
                                                                       gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                          <  3:224598/139‑1 (MQ=255)
                                                                         gCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATg                                                                 >  4:155194/1‑130 (MQ=255)
                                                                         gCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATg                                                                 <  3:155194/130‑1 (MQ=255)
                                                                           cAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGATGTGCAAATTCACGTACTTCAGCACCTTCAATAACCGCATTaag                                                      >  3:88293/1‑137 (MQ=255)
                                                                            aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCa                                                                                      <  6:104756/106‑1 (MQ=255)
                                                                            aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCa                                                                                      >  5:104756/1‑106 (MQ=255)
                                                                            aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGt                                                     >  8:305377/1‑139 (MQ=255)
                                                                            aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGt                                                     >  4:253402/1‑139 (MQ=255)
                                                                            aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGt                                                     >  5:401940/1‑139 (MQ=255)
                                                                                 gattATTCGGGATGCATATCCAAACGACTTTTGTCTGTTAGTCGATTGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTAGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTtata                                                <  2:432680/137‑1 (MQ=255)
                                                                                  attATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAAGCATGTGAGCCGTCGGGACGAAGTGCAAGTTAACGTACTTCAGCACCTTCAATAACCGCATTATGTTTatat                                               >  7:260716/2‑139 (MQ=255)
                                                                                           gcTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGcg                                                                                                                             >  4:420767/2‑51 (MQ=255)
                                                                                           gcTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGcg                                                                                                                             <  3:420767/51‑2 (MQ=255)
                                                                                           gcTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCa                                                           >  4:355777/2‑118 (MQ=255)
                                                                                           gcTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCa                                                           <  3:355777/117‑1 (MQ=255)
                                                                                               cATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGt                                    <  3:253402/137‑1 (MQ=255)
                                                                                                atatCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCATCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTggg                                 >  4:331805/1‑139 (MQ=255)
                                                                                                   tCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgc                              <  7:176394/139‑1 (MQ=255)
                                                                                                   tCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgc                              <  7:182176/139‑1 (MQ=255)
                                                                                                   gccAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgc                              <  3:164438/138‑1 (MQ=255)
                                                                                                     cAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATCGCGGCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCTTTATGTTTATATTGCGGAAAAGTGGGGgcag                            <  7:33512/139‑1 (MQ=255)
                                                                                                       aaCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                          <  4:100699/107‑1 (MQ=255)
                                                                                                       aaCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt                                                          >  3:100699/1‑107 (MQ=255)
                                                                                                       aaCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGt                                    <  1:390101/129‑1 (MQ=255)
                                                                                                       aaCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGt                                    >  2:390101/1‑129 (MQ=255)
                                                                                                       aaCGACTTGTGTCTGTGCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgcagca                          <  8:20380/139‑1 (MQ=255)
                                                                                                        aCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGcagcag                         <  2:403055/139‑1 (MQ=255)
                                                                                                         cGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCAAGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGcagcag                         >  2:100956/1‑138 (MQ=255)
                                                                                                            cTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGtaac                     <  5:213581/139‑4 (MQ=255)
                                                                                                               gtgtCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGAAATTTAACGTACATCCTCACCTTCAAATACCGCATTATGTTTATATTTCGGAAAAGTTGGGGCAGCAGtacacgc                  >  4:164438/1‑133 (MQ=255)
                                                                                                                    tGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTAAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGtttg             >  6:393617/1‑137 (MQ=255)
                                                                                                                      ttCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCCCGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGgttgtt           >  7:412948/1‑139 (MQ=255)
                                                                                                                         gTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTa        <  7:290499/139‑1 (MQ=255)
                                                                                                                           cgatggCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATc      >  6:307260/6‑139 (MQ=255)
                                                                                                                                gCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                                    <  1:64221/56‑1 (MQ=255)
                                                                                                                                gCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACg                                                                                    >  2:64221/1‑56 (MQ=255)
                                                                                                                                gCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAAACGCATTATGTTTATATTTCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGttt  >  5:123336/1‑138 (MQ=255)
                                                                                                                                       |                                                                                                                                  
CGTCAAGAACAACGAGGACATGAGAAGGAACGCGCTCCAGAAATGCGAGTAATTCCCCTTCTGATGTATAAGTGCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATGACCGCATTATGTTTATATTGCGGAAAAGTCGGGGCAGCAGTTATCGTGTTAGTTTTATCATTT  >  CP006881/2188788‑2189053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑