| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,191,463 | 2 bp→CC | coding (533‑534/648 nt) | U712_10980 ← | N‑(5'‑phosphoribosyl)anthranilate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,191,463 | 0 | A | C | 100.0% | 147.2 / NA | 46 | Y178W (TAT→TGG) | U712_10980 | N‑(5'‑phosphoribosyl)anthranilate isomerase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (22/24); total (22/24) | |||||||||||
| * | CP006881 | 2,191,464 | 0 | T | C | 97.8% | 143.2 / NA | 46 | Y178W (TAT→TGG) | U712_10980 | N‑(5'‑phosphoribosyl)anthranilate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (21/24); minor base G (1/0); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTATCTGCCTATTTCATTCGGATATGGATACATATCGATTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCGATTCCGCTGGCAAGGTCAATTCCTGCCGGCTGATATTTCATTAAGTCTGTGATAGTGTCCGGATTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTT > CP006881/2191336‑2191596 || gTATCTGCCTATTTCATTCGGATATGGATACATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATag < 4:448431/139‑2 (MQ=255)gTATCTGCCTATTTCATTCGGATATGGATACATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATag < 4:311003/139‑2 (MQ=255)gTATCTGCCTATTTCATTCGGATATGGATACATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATag < 5:16200/139‑2 (MQ=255) ccTATTTCATTCGGATATGGATACATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATaggcgtg < 4:431576/137‑7 (MQ=255) ccTATTTCATTCGGATATGGATACATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATaggcgtg < 7:120791/137‑7 (MQ=255) tttCATTCGGATATGGATACATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGAtgc > 1:64799/1‑137 (MQ=255) tttCATTCGGATATGGATACATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGAtg < 1:136411/138‑2 (MQ=255) atatGGATACATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGAGCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCGATTTCAATAGGCGTGTGATGCTATCCGGAt > 6:179982/1‑138 (MQ=255) cATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACgccgcc < 7:250890/139‑1 (MQ=255) cATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACgccgcc < 5:2378/139‑1 (MQ=255) atatCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCAcgccgc > 4:241861/1‑137 (MQ=255) atatCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCAcgccgc > 7:35158/1‑137 (MQ=255) atatCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCAcgccgc > 3:53203/1‑137 (MQ=255) atatCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCAcgccgc > 3:21322/1‑137 (MQ=255) tatCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACgccgcc < 4:53203/137‑1 (MQ=255) ttCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATag < 1:187544/105‑2 (MQ=255) ttCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGATGCCATTTCAATag > 2:187544/2‑105 (MQ=255) atCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGc > 7:20314/1‑139 (MQ=255) atCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGc > 8:48344/1‑139 (MQ=255) ggttCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGa < 1:45447/136‑1 (MQ=255) ggttCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGGGATGCTATCCGGATTCACGCCGCCAGCGa < 4:275538/136‑1 (MQ=255) gttCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGAtg > 7:161606/2‑111 (MQ=255) gttCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGAtg < 8:161606/111‑2 (MQ=255) gttCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGAt > 6:87644/2‑138 (MQ=255) aTCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATaaagc > 1:101943/1‑137 (MQ=255) aTCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATaaag > 6:409972/1‑137 (MQ=255) aTCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATaaag < 1:103196/138‑2 (MQ=255) ccTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAagcag > 1:14608/1‑138 (MQ=255) tcttctAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCg < 8:20314/137‑1 (MQ=255) tAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTAcc > 2:372856/1‑138 (MQ=255) cTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCAcg > 2:271391/1‑105 (MQ=255) cTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCAcg < 1:271391/105‑1 (MQ=255) catcaGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAgccgcc > 1:163267/1‑139 (MQ=255) atcaGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAgccgcc < 1:372856/138‑1 (MQ=255) atcaGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGACGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAgccgcc < 8:84025/138‑1 (MQ=255) gATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCctgctg < 8:375163/139‑1 (MQ=255) tCTGAGCTTTTTGTCCGGTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGCTGCTTTCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATa > 3:45375/1‑139 (MQ=255) gATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTc > 2:9665/1‑139 (MQ=255) tttGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGca > 7:97280/1‑138 (MQ=255) cGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGcacacatt < 7:297269/138‑3 (MQ=255) ttCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGa < 1:9665/138‑1 (MQ=255) ttCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGc < 5:87644/139‑1 (MQ=255) cTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCctgctga > 3:154683/1‑104 (MQ=255) cTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCctgctga < 4:154683/105‑2 (MQ=255) ggTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTcc < 8:97280/139‑1 (MQ=255) ggCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCtt > 1:394073/1‑139 (MQ=255) || GTATCTGCCTATTTCATTCGGATATGGATACATATCGATTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCGATTCCGCTGGCAAGGTCAATTCCTGCCGGCTGATATTTCATTAAGTCTGTGATAGTGTCCGGATTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTT > CP006881/2191336‑2191596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |