| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,191,735 | 2 bp→TT | coding (261‑262/648 nt) | U712_10980 ← | N‑(5'‑phosphoribosyl)anthranilate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,191,735 | 0 | C | T | 98.1% | 159.7 / ‑3.2 | 52 | coding (262/648 nt) | U712_10980 | N‑(5'‑phosphoribosyl)anthranilate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (31/20); minor base . (0/1); total (31/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
| * | CP006881 | 2,191,736 | 0 | C | T | 100.0% | 162.4 / NA | 52 | E87E (GAG→GAA) | U712_10980 | N‑(5'‑phosphoribosyl)anthranilate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (31/21); total (31/21) | |||||||||||
GATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAGCGCATCGGCATCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTC > CP006881/2191602‑2191865 || gATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTtcat < 6:261663/139‑1 (MQ=255) gAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCAc > 5:424540/1‑139 (MQ=255) aatcaCAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTg < 6:100033/138‑1 (MQ=255) tcaCAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAg < 6:195946/139‑1 (MQ=255) cacaAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGc > 4:47584/1‑139 (MQ=255) aaGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGaa < 7:48344/139‑1 (MQ=255) aGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATAACCGTGAAGCTGAAt > 1:413446/1‑139 (MQ=255) gTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACg > 6:282636/1‑139 (MQ=255) caacaTTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTc < 6:424540/137‑1 (MQ=255) tatcTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCtt > 1:348446/2‑139 (MQ=255) tatatttAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGGCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAgct > 2:285180/5‑136 (MQ=255) tcTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCttc < 3:47584/138‑2 (MQ=255) aaaGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCGTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACTTCGAGCTTCAAgct > 2:157108/1‑136 (MQ=255) gCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTcg > 3:183476/1‑119 (MQ=255) gCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTcg < 4:183476/120‑2 (MQ=255) tttctTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAAtac < 4:295473/136‑3 (MQ=255) aGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt < 2:348446/138‑1 (MQ=255) aGTGGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt < 4:377026/138‑1 (MQ=255) ttGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGt < 6:52877/139‑1 (MQ=255) tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt > 2:258117/1‑133 (MQ=255) tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt < 1:258117/133‑1 (MQ=255) tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGtctc > 7:315092/1‑139 (MQ=255) tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGtctc > 2:7885/1‑139 (MQ=255) tcttgatgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTAttg > 7:242276/4‑137 (MQ=255) atgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATATGTGACATCGTCTCTATTGATTCa > 7:212658/1‑139 (MQ=255) atgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCa > 7:302110/1‑139 (MQ=255) atgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCa > 7:49407/1‑139 (MQ=255) gAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGCATGACGTCGAGCTTCACGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTaa > 2:153766/1‑138 (MQ=255) gAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTaa > 5:240122/1‑138 (MQ=255) acgcgcCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGCGCAGCAGCATCCGCCGGTTTTTCCTCCCCGTGAAGATGAATGACGTAGCGCTTCAAGCTCGT‑GTCAATACGTGACTTCGTCTCTATTTATTCAATAAc > 4:236920/3‑138 (MQ=255) aaGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATAACCGTGAAGCTGAATGACGTCGAGATTCAAGCTCTT‑GGCAATACGTGACATCGACTCTATTGATTCAATAAc > 8:6557/1‑138 (MQ=255) tCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAAcacc > 5:118448/1‑139 (MQ=255) ttCACAGCCTGTCAGCTTGCGAAGCGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATGCATTAACAAAAACACCGGCAAcc > 7:397797/1‑139 (MQ=255) agccTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAAc < 8:140074/130‑1 (MQ=255) agccTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATAACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAAc > 7:140074/4‑133 (MQ=255) ccTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCttc > 5:281143/2‑71 (MQ=255) ccTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCttc < 6:281143/71‑2 (MQ=255) tGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACTGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTc > 5:335189/1‑138 (MQ=255) tGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTc > 7:391604/1‑138 (MQ=255) gTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGttttt > 6:123200/1‑136 (MQ=255) gTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGttttt < 5:123200/136‑1 (MQ=255) gTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa > 1:229942/1‑139 (MQ=255) tCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAGGCTCTT‑GGCATTACGTGACATCGTCTCGATTGATTCATTAGCAAAAACACCTGCACCCTGTTTTTCGac > 2:287172/1‑139 (MQ=255) tCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa < 2:413446/138‑1 (MQ=255) tCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTT‑TTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa < 8:170366/137‑1 (MQ=255) tCAGCTTGCGAACAGCAGCAGCATCCGCCTGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa < 6:13699/138‑1 (MQ=255) aGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGATGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGacac < 1:157108/139‑1 (MQ=255) aGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGacac < 8:391604/139‑1 (MQ=255) tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt > 4:370674/1‑139 (MQ=255) tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt > 1:250719/1‑139 (MQ=255) tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt > 1:226304/1‑139 (MQ=255) cGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTc < 6:118448/139‑1 (MQ=255) || GATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAGCGCATCGGCATCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTC > CP006881/2191602‑2191865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |