| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,192,543 | 2 bp→CC | coding (210‑211/753 nt) | U712_10985 ← | Indole‑3‑glycerol phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,192,543 | 0 | T | C | 97.6% | 128.3 / NA | 41 | T71A (ACT→GCT) | U712_10985 | Indole‑3‑glycerol phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (25/15); minor base A (1/0); total (26/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | CP006881 | 2,192,544 | 0 | T | C | 100.0% | 132.7 / NA | 41 | E70E (GAA→GAG) | U712_10985 | Indole‑3‑glycerol phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/15); total (26/15) | |||||||||||
GAAGAGAATCAATAATAAAATCTTTTCTGAGTACAGGTATCGAAACAGCGCGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAAGCGCATCTGCTTTCGCAGTTTCATAGTCTTTTGCAATCCGCACAGGTACAAAATTCTCTTTAATAAGCCCTTTTGACGGCGACGCTTTCTTCACTTCAGCAATCAACCCGATAAACCGATTCAGGCTTGCCAGCGCCTCTTTAAATGAAC > CP006881/2192411‑2192674 || cttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATa > 2:150623‑M1/14‑139 (MQ=255) agaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACACCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCAGCTGCCTTCGCAGCCTCATAGTCtttt > 5:84648‑M1/7‑139 (MQ=255) gaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTg > 1:290927‑M1/6‑139 (MQ=255) atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCTTAGTCTTTTGCa > 2:25867‑M1/4‑139 (MQ=255) atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCa > 8:91228‑M1/4‑139 (MQ=255) gaaTCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGTTAAATAGCTGTTTTGCCCTTGAATAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATctgga < 3:165207/138‑5 (MQ=255) AAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCa > 5:411810‑M1/1‑139 (MQ=255) aaTCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGcac < 7:219317/139‑1 (MQ=255) aTCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGca > 4:23488/1‑137 (MQ=255) ttCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAg < 7:305919/135‑1 (MQ=255) ttCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAg < 5:363723/135‑1 (MQ=255) aGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACaaa < 3:217563/137‑1 (MQ=255) aGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAAt < 1:200015/139‑1 (MQ=255) tCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAAAGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTtaat < 4:224844/139‑1 (MQ=255) gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTtaataa < 1:187186/139‑1 (MQ=255) gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTtaat > 6:230830/1‑137 (MQ=255) gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCAAAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTtaat > 6:122562/1‑137 (MQ=255) cacgcTTTACTTATGATAAATAGCTGTATTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATAGAATCTGCCTTCGCCGACTCATAGTCTTATGCAATATGCACAGGTACATAATACACTATAATAAGCCCttt > 4:204546/3‑139 (MQ=255) cacgcTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCttt > 7:199455/3‑139 (MQ=255) cacgcTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCttt > 7:318044/3‑139 (MQ=255) tttACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATc > 8:192027/1‑99 (MQ=255) tttACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATc < 7:192027/99‑1 (MQ=255) tttACGTCTGATAAATAGCTGTTTTCCACTTGAAAAAACGGGGTGTCTGTTAAAACGGACATCGCATCTGACTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACg > 8:210062/1‑139 (MQ=255) aTAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCttt > 1:311186/1‑139 (MQ=255) aTAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCttt > 1:369375/1‑139 (MQ=255) aTAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCttt > 7:9858/1‑139 (MQ=255) aTAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCttt > 7:173472/1‑139 (MQ=255) aTAAATAGATGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATACGCCCGTTTGACGTCGATCCttt > 8:122583/1‑139 (MQ=255) ttttCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCa < 5:193158/75‑1 (MQ=255) ttttCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCa > 6:193158/1‑75 (MQ=255) cTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCaa < 5:217120/139‑1 (MQ=255) gAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGCCGGCGATGCTTTCTTCACTTCGGCAATCAAccc > 5:67261/1‑139 (MQ=255) gAAAAAACGGGGTGTCTGGTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAAc < 3:23488/137‑1 (MQ=255) gtgtCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATcctc > 7:239827/1‑77 (MQ=255) gtgtCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATcctc < 8:239827/77‑1 (MQ=255) gAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCACCCCGATAAACCGATTCGGGCTTGCCAgcg > 8:175337/1‑139 (MQ=255) aaaTCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAgcgc < 2:369375/139‑1 (MQ=255) aaaTCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAgcgc < 5:165706/139‑1 (MQ=255) tCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTa > 7:140776/1‑138 (MQ=255) tCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTa > 8:214390/1‑138 (MQ=255) ttCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAAc > 7:233413/1‑139 (MQ=255) || GAAGAGAATCAATAATAAAATCTTTTCTGAGTACAGGTATCGAAACAGCGCGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAAGCGCATCTGCTTTCGCAGTTTCATAGTCTTTTGCAATCCGCACAGGTACAAAATTCTCTTTAATAAGCCCTTTTGACGGCGACGCTTTCTTCACTTCAGCAATCAACCCGATAAACCGATTCAGGCTTGCCAGCGCCTCTTTAAATGAAC > CP006881/2192411‑2192674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |