Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,192,702	2 bp→GA	coding (51‑52/753 nt)	U712_10985 ←	Indole‑3‑glycerol phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,192,702	0	A	G	100.0%	193.6 / NA	60	L18L (TTG→CTG)	U712_10985	Indole‑3‑glycerol phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/36);  total (24/36)
*	CP006881	2,192,703	0	G	A	100.0%	187.2 / NA	60	V17V (GTC→GTT)	U712_10985	Indole‑3‑glycerol phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/36);  total (24/36)

GGTACAAAATTCTCTTTAATAAGCCCTTTTGACGGCGACGCTTTCTTCACTTCAGCAATCAACCCGATAAACCGATTCAGGCTTGCCAGCGCCTCTTTAAATGAACGTTTTTCGAAAGGCTGCTCTTCCGGCAAGACCAGTGTTTTTACTTCTTCTTTCTTTTGTTTAATGA‑TGTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTCTCTAATGCTAGCTCCG  >  CP006881/2192569‑2192834                                                                                                                                      ||                                                                                                                                     ggTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCa                                                                                                                                  <  2:293219/139‑1 (MQ=255) ggTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCa                                                                                                                                  <  2:193408/139‑1 (MQ=255)      aaaaTCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGtt                                                                                                                             <  8:173472/139‑1 (MQ=255)       aaaTCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGttt                                                                                                                            >  1:27177/1‑139 (MQ=255)            ctctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCAct                                                                                                                       >  5:413382/1‑139 (MQ=255)            ctctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCAct                                                                                                                       >  8:142133/1‑139 (MQ=255)            ctctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTAGCAAGGCTGCTCTACCGGCAGAACCAGTGttttccct                                                                                                                       >  5:391714/1‑135 (MQ=255)            ctctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATACGGGCTTGCAACCGCCTCATTAAATGAACGTTTCTCGAAAGCCTGCTCTACCGGCAGAACCACGGtttacact                                                                                                                       >  5:6952/1‑134 (MQ=255)             tctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCActt                                                                                                                      >  2:234921/1‑139 (MQ=255)               ttttATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCActt                                                                                                                      <  8:307690/137‑1 (MQ=255)               tttAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCActt                                                                                                                      <  8:233413/137‑1 (MQ=255)                           ttttGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGt                                                                                                        >  3:385281/1‑139 (MQ=255)                                  ggCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTtgatga                                                                                                 >  6:98294/1‑139 (MQ=255)                                  ggCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTtgatga                                                                                                 >  4:170491/1‑139 (MQ=255)                                     gatgCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTgatga‑ttt                                                                                             >  2:321489/4‑137 (MQ=255)                                         cTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTc                                                                                         <  1:321489/139‑1 (MQ=255)                                         cTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTc                                                                                         <  3:88134/139‑1 (MQ=255)                                             cttcacttcgGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAg                                                                                      <  2:27177/138‑1 (MQ=255)                                                cacttcgGCAATCAACCCGCTAAACCGATTCGGGCTTGACAGCGACTCCTTAAATGTACGTTTCTCGAAAGGCTGCTCTCCCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTTATGA‑TTTTTTCAAGCa                                                                                    <  7:122583/137‑1 (MQ=255)                                                cacttcgGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCa                                                                                    <  1:291527/137‑1 (MQ=255)                                                cacttcgGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCAt                                                                                   <  4:385281/138‑1 (MQ=255)                                                       gCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATctct                                                                           <  8:318044/139‑1 (MQ=255)                                                        cAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCtt                                                                          <  7:314101/139‑1 (MQ=255)                                                             aaCCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCctct                                                                     >  5:147555/1‑139 (MQ=255)                                                              aCCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCtt                                                                    <  4:126848/139‑1 (MQ=255)                                                                ccGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTGTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTc                                                                  <  2:311186/139‑1 (MQ=255)                                                                         cGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGt                                                         <  6:67261/139‑1 (MQ=255)                                                                         cGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGt                                                         <  6:6952/139‑1 (MQ=255)                                                                          gATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCttt                                                                   <  5:326030/128‑1 (MQ=255)                                                                          gATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTc                                                        >  1:196190/1‑139 (MQ=255)                                                                          gATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTATTCCTCttt                                                                   >  6:326030/1‑128 (MQ=255)                                                                           aTTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCa                                                                                    <  7:137127/110‑1 (MQ=255)                                                                           aTTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCa                                                                                    <  3:117114/110‑1 (MQ=255)                                                                           aTTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCa                                                                                    >  8:137127/1‑110 (MQ=255)                                                                           aTTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCa                                                                                    >  4:117114/1‑110 (MQ=255)                                                                                ggCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCa                                                   >  6:83135/1‑138 (MQ=255)                                                                                 gCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAg                                                 <  7:214390/139‑1 (MQ=255)                                                                                 gCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGGCGTTCAAg                                                 <  7:142133/139‑1 (MQ=255)                                                                                     gCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGATTTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTg                                              >  4:135452/1‑139 (MQ=255)                                                                                     gCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTgc                                             >  6:218920/1‑139 (MQ=255)                                                                                      ccAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTgcg                                            >  6:628/1‑139 (MQ=255)                                                                                          cgcCTCCTTAACTGAACGTTTCGCGATAGGCTGCTCTACCGGCAGAACGAGTGTTTTCACTTCTGCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCTTAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGc                                        <  4:416204/139‑1 (MQ=255)                                                                                            cctcctTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTGTCTGCTTTAGTCGTTCAAGCTGCGCGGCAg                                      >  2:362189/1‑139 (MQ=255)                                                                                             ctccTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAgc                                     <  5:83135/139‑1 (MQ=255)                                                                                               ccttAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTAATGCTTAGATATCTTCCTCGTTCTGCTTTAGTCGTTCAAGCTGCTCGGCAgcgc                                   >  5:67825/3‑139 (MQ=255)                                                                                                cttAAATGAACGTCTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGcc                                  <  2:271100/138‑1 (MQ=255)                                                                                                   aaaTGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCt                                 <  6:413382/137‑1 (MQ=255)                                                                                                   aaaTGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTc                                <  5:628/138‑1 (MQ=255)                                                                                                          cGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATATCTTCCTCTTTCTGCTTTAGTCTTTCAAACTTCCCGGCAGCCCCACCGCttct                         >  2:436079/1‑136 (MQ=255)                                                                                                            tttctCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGAGCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAAt                      <  5:230830/139‑1 (MQ=255)                                                                                                                   aaaGGCTGCTCTACCGGCAGATCCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTGTGCTTTAGTGTTTCATGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTctct               <  1:436079/139‑1 (MQ=255)                                                                                                                   aaaGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTctct               <  6:372998/139‑1 (MQ=255)                                                                                                                   aaaGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTctct               <  6:147555/139‑1 (MQ=255)                                                                                                                    aaGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGATTTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTctct               <  3:135452/139‑1 (MQ=255)                                                                                                                    aaGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCttt                                                                   >  1:1677/1‑86 (MQ=255)                                                                                                                    aaGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCttt                                                                   <  2:1677/86‑1 (MQ=255)                                                                                                                           ctctACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCctct                                                                     >  4:432061/1‑77 (MQ=255)                                                                                                                           ctctACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCctct                                                                     <  3:432061/77‑1 (MQ=255)                                                                                                                                ccGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTCTCTAACGCCAGCt     <  8:199455/136‑1 (MQ=255)                                                                                                                                ccGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTCTCTAACGCCAGCTCCg  <  3:310574/139‑1 (MQ=255)                                                                                                                                      ||                                                                                                                                     GGTACAAAATTCTCTTTAATAAGCCCTTTTGACGGCGACGCTTTCTTCACTTCAGCAATCAACCCGATAAACCGATTCAGGCTTGCCAGCGCCTCTTTAAATGAACGTTTTTCGAAAGGCTGCTCTTCCGGCAAGACCAGTGTTTTTACTTCTTCTTTCTTTTGTTTAATGA‑TGTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTCTCTAATGCTAGCTCCG  >  CP006881/2192569‑2192834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑