Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,194,850 2 bp→GT coding (431‑432/1548 nt) U712_10995 ← Anthranilate synthase component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,194,8500TG98.2% 166.0 / NA 54G144D (GGA→GAC) U712_10995Anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (32/21);  minor base C (0/1);  total (32/22)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0068812,194,8510CT98.2% 164.8 / NA 54G144D (GGA→GAC) U712_10995Anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (31/22);  minor base G (1/0);  total (32/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACATCGATTTTTTCGTTTTCTGTTTCCTGTCCATTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTAGTTTCATGATCATATGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATTCCTGTTTCTTTTGAATGCGGAGGAACAGAAGGCTCAATCAGCGGGATCATATCGTAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCTAGCTCTGGCGTTTTGATTTTGTACGTGGTATTCATC  >  CP006881/2194724‑2194982
                                                                                                                              ||                                                                                                                                   
tACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACCCTTTTCCATGTCTGTTTCtttg                                                                                                                          >  3:386367/1‑138 (MQ=255)
        ttttttCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTGATGAACATACGCAATTTAATTCCGGCAAACAAAAAACAAACACTTTTCCATGTGTGTTTATTTTGGAttgg                                                                                                                   >  4:251688/1‑135 (MQ=255)
        ttttttCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCg                                                                                                                   >  6:379057/1‑138 (MQ=255)
                tcttttGTATCCTCTCCAGTGAGCCTTTCATATTGGATAAAGTGGCCGAGTTTGGATTCATGATCATACCCAATTAATGTCCGGCAAACAAACCGCAAACACTTATCCATCTCTGTTTCTTTGGTAAGCGAAGGAACAg                                                                                                          <  5:13803/134‑1 (MQ=255)
                                cagtGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAg                                                                                             <  2:179382/133‑1 (MQ=255)
                                   tGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGa                                                                                        <  3:251688/138‑1 (MQ=255)
                                   tGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGAt                                                                                       <  6:382886/139‑1 (MQ=255)
                                   tGAGCCTTGCATATTGGATAAAGTGGACGTGGTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACACCATACACTTTTCAATGTCTGTTTCTTGGGTATGCGAAGGATCACAACGCGAACTCAGCGGGAt                                                                                       >  5:129394/1‑139 (MQ=255)
                                     aGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAAATAATGTCCGGCAAACAAACAGCTTACACTTTTCCATGTCTGTTTCTTTGGTTTGCGAAGGAACAGAAGGCTCAATCAGCGGTATc                                                                                      <  5:31447/138‑1 (MQ=255)
                                       ccTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAg                                                                                                          >  5:117076/1‑116 (MQ=255)
                                       ccTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAg                                                                                                          <  6:117076/116‑1 (MQ=255)
                                           gCATATTGGATAAAGTGGACGTTTTTGGTTGCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATca                                                                                <  1:107268/138‑2 (MQ=255)
                                                ttGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCtt                                                                          <  5:379057/139‑1 (MQ=255)
                                                 tGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGa                                                                                        >  6:18838/1‑124 (MQ=255)
                                                 tGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGa                                                                                        <  5:18838/124‑1 (MQ=255)
                                                 tGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGGCGGTTTCTTTGGTATGCGTAGGAACAGAAGGCTCATTCAGCGGGATCATATCATAGGtt                                                                          >  1:206305/1‑138 (MQ=255)
                                                 tGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTACATGTGTGTTTCTTTGGTATGCGAATGAACAGAAGGCTCAATCAGCGGGATAATATCATAGAtt                                                                          >  1:398069/1‑138 (MQ=255)
                                                        aGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTAccc                                                                  >  5:400834/1‑139 (MQ=255)
                                                        aGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTAccc                                                                  >  3:99233/1‑139 (MQ=255)
                                                             aCGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCTGCGGGATCATATCATAGCTTAAGTACCCGACAg                                                             >  1:402536/1‑139 (MQ=255)
                                                             aCGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAg                                                                                             <  7:442972/107‑1 (MQ=255)
                                                             aCGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAg                                                                                             >  8:442972/1‑107 (MQ=255)
                                                             aCGTTTTTGGGTTCATGCTCATACGCAATTAATGTGCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCTGACAg                                                             >  4:431393/1‑139 (MQ=255)
                                                                  tttGGTTTCAAGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCCTGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTAACCGACAGCTccg                                                        >  3:317114/1‑139 (MQ=255)
                                                                    tggTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTccgc                                                       <  6:119233/136‑1 (MQ=255)
                                                                       tttCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGa                                                    >  4:168340/1‑138 (MQ=255)
                                                                          cATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGAt                                                                                       >  8:101488/1‑100 (MQ=255)
                                                                          cATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGAt                                                                                       <  7:101488/100‑1 (MQ=255)
                                                                           aTGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACaa                                                 >  7:206081/1‑137 (MQ=255)
                                                                                      cAATTAATGTCGGGAAAAAAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAg                                    <  4:255158/139‑1 (MQ=255)
                                                                                         ttAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATca                                                                                >  6:402057/1‑91 (MQ=255)
                                                                                         ttAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATca                                                                                <  5:402057/92‑2 (MQ=255)
                                                                                         ttAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGct                                  >  6:279406/1‑138 (MQ=255)
                                                                                           aaTGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGctca                                <  6:301578/138‑2 (MQ=255)
                                                                                            aTGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGctcag                               <  4:85370/138‑3 (MQ=255)
                                                                                                 cGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCTAAGGAACAGAAGGCTCAATCAGCGGGAGCATATCATAGCTTAAGTACACGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGttt                         >  2:323907/1‑139 (MQ=255)
                                                                                                 cGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGc                                                                                            <  1:174469/72‑1 (MQ=255)
                                                                                                 cGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGc                                                                                            >  2:174469/1‑72 (MQ=255)
                                                                                                 cGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCCAGCTCAGGTGttt                         >  6:244051/1‑139 (MQ=255)
                                                                                                 cGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGttt                         >  3:255158/1‑139 (MQ=255)
                                                                                                 cGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGttt                         >  3:57567/1‑139 (MQ=255)
                                                                                                 cGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGttt                         >  5:16647/1‑139 (MQ=255)
                                                                                                 cGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGttt                         >  7:315365/1‑139 (MQ=255)
                                                                                                          aacaGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGt                <  8:206081/139‑1 (MQ=255)
                                                                                                              gCATACACTTATCCTTGTCTGTTGCTTTGGTATGCGAATGAACAGAAGGCTCAATCAGCGGTATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGt            <  2:309692/139‑1 (MQ=255)
                                                                                                                       tttCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGCCAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCAt   >  1:262393/1‑139 (MQ=255)
                                                                                                                       tttCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCAt   >  7:102235/1‑139 (MQ=255)
                                                                                                                         tCCATGTCTGTTTCTTTGGTATGCGAATGAACAGATGGCTAAATCAGCGGGTTCATATCATAGCTTATGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATc  <  4:336903/138‑1 (MQ=255)
                                                                                                                         tCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAg                                                                                             <  7:202681/47‑1 (MQ=255)
                                                                                                                         tCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAg                                                                                             >  8:202681/1‑47 (MQ=255)
                                                                                                                         tCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATc  >  1:340110/1‑138 (MQ=255)
                                                                                                                         tCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATc  >  5:28803/1‑138 (MQ=255)
                                                                                                                         tCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATc  >  4:283269/1‑138 (MQ=255)
                                                                                                                         tCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGGGTTTTGATTTTGTATGTGGTATTCATc  <  4:386367/138‑1 (MQ=255)
                                                                                                                              ||                                                                                                                                   
TACATCGATTTTTTCGTTTTCTGTTTCCTGTCCATTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTAGTTTCATGATCATATGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATTCCTGTTTCTTTTGAATGCGGAGGAACAGAAGGCTCAATCAGCGGGATCATATCGTAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCTAGCTCTGGCGTTTTGATTTTGTACGTGGTATTCATC  >  CP006881/2194724‑2194982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑