Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,173,929	2 bp→CG	coding (996‑997/1194 nt)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,173,929	0	T	C	100.0%	148.0 / NA	47	T333A (ACT→GCT)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/29);  total (18/29)
*	CP006881	2,173,930	0	A	G	100.0%	152.8 / NA	47	N332Y (AAT→TAC)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/29);  total (18/29)

CCTGCTCGATCCGCTGTAATTCTTCCGACACCCATTTTCCGGCAGGCCGGTTACGAAGCGCTAGTAAATCTTTACCCGTGATATCAAGATCCCTAAGGCTTTTGATCGGCAGGTTTTGATAAGTATTTTGAATGTCCTTTAGTTTTTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAAAGAAGTGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCT  >  CP006881/2173806‑2174049                                                                                                                            ||                                                                                                                        ccTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCtt                                                                                                           <  1:268091/139‑1 (MQ=255) ccTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCtt                                                                                                           <  4:199180/139‑1 (MQ=255)    gCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAg                                                                                                        >  1:306096/1‑139 (MQ=255)    gCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAg                                                                                                        >  2:18396/1‑139 (MQ=255)     cTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGt                                                                                                       <  5:440005/139‑1 (MQ=255)      tCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGtt                                                                                                      >  8:132528/1‑139 (MQ=255)                         ctgACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTc                                                                                   >  6:198396/3‑139 (MQ=255)                         ctgACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTc                                                                                   >  7:392584/3‑139 (MQ=255)                                              gCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGatat                                                              >  6:253246/1‑139 (MQ=255)                                              gCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGatat                                                              >  7:308515/1‑139 (MQ=255)                                                   tttcGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTgg                                                         <  4:412317/136‑1 (MQ=255)                                                        aaGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCt                                                    <  7:253740/139‑1 (MQ=255)                                                                       cttACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt                                      <  8:182370/137‑1 (MQ=255)                                                                       cttACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt                                      <  2:306096/137‑1 (MQ=255)                                                                       cttACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt                                      <  8:392584/137‑1 (MQ=255)                                                                          aCCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt                                      <  6:62259/135‑1 (MQ=255)                                                                          aCCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt                                      >  5:62259/1‑135 (MQ=255)                                                                           cccGTGATATGAAGATCCTTAAGGATTTTGATAGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTtcc                                 <  7:322641/139‑1 (MQ=255)                                                                           cccGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTtcc                                 <  2:302637/139‑1 (MQ=255)                                                                           cccGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTtcc                                 <  5:198396/139‑1 (MQ=255)                                                                           cccGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTGGCCGAAGCTGCGATATTTTGGCCGCTGAGATCAGTGCTTTTTtcc                                 <  5:11784/139‑1 (MQ=255)                                                                           cccGTGATATCAAGATCCTTAAGGCGTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTtcc                                 <  3:134381/139‑1 (MQ=255)                                                                               tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCt                                                    >  4:35238/1‑116 (MQ=255)                                                                               tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCt                                                    <  3:35238/116‑1 (MQ=255)                                                                               tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc                             >  8:2406/1‑139 (MQ=255)                                                                               tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc                             >  1:112670/1‑139 (MQ=255)                                                                               tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc                             >  6:66793/1‑139 (MQ=255)                                                                               tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc                             >  1:331649/1‑139 (MQ=255)                                                                                gATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt                            <  5:253246/139‑1 (MQ=255)                                                                                gATATCAAGATCCTTAAGGCTTTTGATCGCCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAg                                                                       >  3:160686/1‑96 (MQ=255)                                                                                gATATCAAGATCCTTAAGGCTTTTGATCGCCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAg                                                                       <  4:160686/96‑1 (MQ=255)                                                                                 atttCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTGAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt                            <  4:422298/135‑1 (MQ=255)                                                                                 atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATGGAGTTTTTCGTTTTGCCGATGCTGCGTTATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCtg                           <  5:140245/139‑2 (MQ=255)                                                                                 atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCtc                           <  3:422123/139‑2 (MQ=255)                                                                                 atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt                            <  7:132528/138‑1 (MQ=255)                                                                                 atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt                            <  4:66477/138‑1 (MQ=255)                                                                                 atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAAAGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCtc                           <  6:204147/139‑2 (MQ=255)                                                                                      aaGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTa                       <  1:18396/138‑1 (MQ=255)                                                                                              tAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTg                         >  7:209914/1‑128 (MQ=255)                                                                                              tAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTg                         <  8:209914/128‑1 (MQ=255)                                                                                               aaGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt              <  2:415330/138‑1 (MQ=255)                                                                                               aaGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt              <  2:112670/138‑1 (MQ=255)                                                                                                     tttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc        >  5:393025/1‑138 (MQ=255)                                                                                                      ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc        >  5:259403/1‑137 (MQ=255)                                                                                                      ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc        >  3:271622/1‑137 (MQ=255)                                                                                                      ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCa       <  5:66793/138‑1 (MQ=255)                                                                                                          tCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGct  <  4:271622/139‑1 (MQ=255)                                                                                                                            ||                                                                                                                        CCTGCTCGATCCGCTGTAATTCTTCCGACACCCATTTTCCGGCAGGCCGGTTACGAAGCGCTAGTAAATCTTTACCCGTGATATCAAGATCCCTAAGGCTTTTGATCGGCAGGTTTTGATAAGTATTTTGAATGTCCTTTAGTTTTTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAAAGAAGTGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCT  >  CP006881/2173806‑2174049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑