Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,195,545 2 bp→AA coding (335‑336/384 nt) U712_11000 ← Chorismate mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,195,5450GA99.1% 336.1 / NA 107A112V (GCC→GTT) U712_11000Chorismate mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (42/64);  minor base T (0/1);  total (42/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0068812,195,5460GA99.1% 330.7 / NA 107A112V (GCC→GTT) U712_11000Chorismate mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (42/64);  minor base T (0/1);  total (42/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TCTCTGCTACCCTATTCTCTAACTCAACTCATTC‑AATCGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCCTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGTCTTAATACAACGGCTTTTTCTAAATATACATGCCTGATCCGTTCCTGAGGAACATCTGTCTGGACGGTCATCATGACTCTGATGCACTTTTTCAGACCGCCGGTTACGTCCATTTCCTGCATACAGGTTACCGGCACA  >  CP006881/2195415‑2195671
                                                                                                                                   ||                                                                                                                             
tctcTGCTACCCTATTCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCATTATTTTTTGTAAATGATAAATCGGGCCTCAATACGACAACtttt                                                                                                                          <  2:328500/138‑1 (MQ=255)
tctcTGCTACCCTATTCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACtttt                                                                                                                          >  8:331903/1‑138 (MQ=255)
 ctctGCTTCCCTATTCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACttttt                                                                                                                         <  3:76181/138‑1 (MQ=255)
 ctctGCTACCCTATTCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATACAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACttttt                                                                                                                         <  6:11845/138‑1 (MQ=255)
   ctGCTACCCTATTCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCt                                                                                                                       >  3:77178/1‑138 (MQ=255)
    tGCTACCCTATTCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCt                                                                                                                       <  4:305629/137‑1 (MQ=255)
    tGCTACCCTATTCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCt                                                                                                                       <  3:138686/137‑1 (MQ=255)
         cccTATTCTCTATCTCAACTCATTCTAATTGCTTTATACAGACTCTTGTGTTTTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGTCAACTTTTTCTAAAtata                                                                                                                <  7:138975/139‑1 (MQ=255)
         cccTATTCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAata                                                                                                                  <  1:114013/137‑1 (MQ=255)
         cccTATTCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAAtata                                                                                                                <  3:2379/139‑1 (MQ=255)
         cccTATTCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAAtata                                                                                                                <  7:82789/139‑1 (MQ=255)
            tATTCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACAt                                                                                                             >  4:186780/1‑139 (MQ=255)
              ttCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACttttt                                                                                                                         <  5:343776/125‑1 (MQ=255)
              ttCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATgt                                                                                                           <  6:179258/139‑2 (MQ=255)
              ttCTCTAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATgt                                                                                                           >  4:421109/1‑138 (MQ=255)
                   tAACTCAACTCATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGAt                                                                                                      <  4:77178/139‑1 (MQ=255)
                    aactcaactcATTCTAATTGCTTTATACACACTCTTGTGTATTTCTAATCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATc                                                                                                     <  6:78315/139‑1 (MQ=255)
                     actcaactcaTTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGAt                                                                                                      <  3:421109/137‑1 (MQ=255)
                     actcaactcaTTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACAAGTCTGAt                                                                                                      <  4:237381/137‑1 (MQ=255)
                      ctcaactcaTTCTAATTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATct                                                                                                    >  4:325250/1‑137 (MQ=255)
                               ttctaaTTGCTTTTTACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAgg                                                                                          <  5:333366/135‑1 (MQ=255)
                               ttctaaTTGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAgg                                                                                          <  1:365273/135‑1 (MQ=255)
                                    attgCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACAt                                                                                     <  3:440788/136‑1 (MQ=255)
                                          ttATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACAtctgtct                                                                               >  7:277761/1‑139 (MQ=255)
                                          ttATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACAt                                                                                     <  4:87489/133‑1 (MQ=255)
                                          ttATACACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACAt                                                                                     >  3:87489/1‑133 (MQ=255)
                                             taCACACTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGa                                                                            <  7:331903/139‑1 (MQ=255)
                                                 cacTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGt                                                                        <  1:317271/139‑1 (MQ=255)
                                                  acTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACTACAACTTTTTCTAAATATACATTTATGATCTGATCCTGAGGGACATCTGTCTGGACCGt                                                                        >  3:273514/1‑138 (MQ=255)
                                                  acTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTggcccgt                                                                        >  4:104516/1‑133 (MQ=255)
                                                  acTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGa                                                                            >  6:425605/1‑134 (MQ=255)
                                                  acTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGa                                                                            <  5:425605/134‑1 (MQ=255)
                                                  acTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGtc                                                                       >  6:6208/1‑139 (MQ=255)
                                                  acTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGt                                                                        >  7:175973/1‑138 (MQ=255)
                                                  acTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGt                                                                        >  8:74098/1‑138 (MQ=255)
                                                  acTCTTGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGt                                                                        >  2:145387/1‑138 (MQ=255)
                                                      ttGTGTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGtcat                                                                     <  7:74098/137‑1 (MQ=255)
                                                       tgtgTATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTcatcat                                                                  >  1:361199/1‑139 (MQ=255)
                                                        gtgtATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTcatcat                                                                  <  8:277761/138‑1 (MQ=255)
                                                        gtgtATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTcatcat                                                                  <  7:86834/138‑1 (MQ=255)
                                                         tgtATTTCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGa                                                                <  5:33664/139‑1 (MQ=255)
                                                         tgtATTTCTAAGCTGTTCTTATAGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGAACGTCATCATGa                                                                <  5:27681/139‑1 (MQ=255)
                                                              ttCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATgtc                                                                                                          >  3:279549/1‑90 (MQ=255)
                                                              ttCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATgtc                                                                                                          <  4:279549/92‑3 (MQ=255)
                                                              ttCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACa                                                                                      <  4:426464/112‑1 (MQ=255)
                                                              ttCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACa                                                                                      >  3:426464/1‑112 (MQ=255)
                                                              ttCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGAgtct                                                            >  4:110466/1‑138 (MQ=255)
                                                              ttCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGActctt                                                           >  6:411029/1‑138 (MQ=255)
                                                              ttCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGActct                                                            >  8:188048/1‑138 (MQ=255)
                                                              ttCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGActct                                                            >  1:358976/1‑138 (MQ=255)
                                                              ttCTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGActct                                                            >  4:428782/1‑138 (MQ=255)
                                                                cTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCt                                                                                                                       <  1:356800/77‑1 (MQ=255)
                                                                cTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTAt                                                         >  4:142533/1‑139 (MQ=255)
                                                                cTAAGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTAt                                                         >  3:177380/1‑139 (MQ=255)
                                                                   atcTGTTCTTTTCGTATAACCATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCa                                                      <  6:297027/137‑1 (MQ=255)
                                                                   aGCTGTTCTTATCGTATTTCAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATg                                                        <  3:189118/137‑1 (MQ=255)
                                                                   aGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATg                                                        <  5:4062/137‑1 (MQ=255)
                                                                   aGCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGGCTGGACCGTCATCATGACTCTTATGCa                                                      <  7:76004/139‑1 (MQ=255)
                                                                   aGCTGTTCTTATCGTATTACAATTCACTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATg                                                        <  3:6226/137‑1 (MQ=255)
                                                                    gCTGTTCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCAc                                                     <  5:411029/139‑1 (MQ=255)
                                                                        ttCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACtttt                                                 >  5:165317/1‑139 (MQ=255)
                                                                        ttCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACtttt                                                 >  3:196212/1‑139 (MQ=255)
                                                                        ttCTTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACGTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACtttt                                                 >  1:293798/1‑139 (MQ=255)
                                                                              tCGTATTACTATTAAGTATTTTTTGTCAATGATAAATCGGGCCTCTATACGACTACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGAc                                           <  4:273514/139‑1 (MQ=255)
                                                                                 tATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGAccgc                                        <  7:188048/139‑1 (MQ=255)
                                                                                  aTTACAATTCAGTATTTTTTGTCAATGATAAATCGTGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCATGAGGGCCATCTGTCTGTACCGTCATCATGACTCTTATGCACTTTTTAAGAcagag                                       >  7:175356/1‑135 (MQ=255)
                                                                                  aTTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGAccgcc                                       >  4:293422/1‑139 (MQ=255)
                                                                                  aTTACAATTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGAccgcc                                       >  5:429594/1‑139 (MQ=255)
                                                                                       aaTTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTa                                  <  4:196212/139‑1 (MQ=255)
                                                                                       aaTTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTa                                  <  4:177380/139‑1 (MQ=255)
                                                                                       aaTTCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTa                                  <  3:186780/139‑1 (MQ=255)
                                                                                         ttCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACAt                                                                                     >  2:9654/1‑86 (MQ=255)
                                                                                         ttCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACAt                                                                                     <  1:9654/86‑1 (MQ=255)
                                                                                         ttCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACg                                >  8:425989/1‑139 (MQ=255)
                                                                                         ttCAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACg                                <  5:84797/139‑1 (MQ=255)
                                                                                           cAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGa                                                                <  6:3654/105‑1 (MQ=255)
                                                                                           cAGTATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGa                                                                >  5:3654/1‑105 (MQ=255)
                                                                                              tATTTTTTGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCAt                           >  3:184988/1‑139 (MQ=255)
                                                                                                  ttttGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTc                        <  5:346066/138‑1 (MQ=255)
                                                                                                  ttttGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTc                        <  6:429594/138‑1 (MQ=255)
                                                                                                  ttttGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTc                        <  8:3864/138‑1 (MQ=255)
                                                                                                  ttttGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTc                        <  6:165317/138‑1 (MQ=255)
                                                                                                  ttttGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTc                        <  8:175973/138‑1 (MQ=255)
                                                                                                  ttttGTCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTc                        <  4:12029/138‑1 (MQ=255)
                                                                                                  ttttGTCAATGATAAATCGGGCCTCAATACGACAACATTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTc                        <  2:293798/138‑1 (MQ=255)
                                                                                                  ttttGGCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTc                        <  1:145387/138‑1 (MQ=255)
                                                                                                       tCAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCa                   <  7:7245/138‑1 (MQ=255)
                                                                                                        cAATGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATa                 >  3:131125/1‑139 (MQ=255)
                                                                                                         aaTGATAAATCGTGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATa                 <  6:64691/138‑1 (MQ=255)
                                                                                                         aaTGATAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATAc                <  8:375637/139‑1 (MQ=255)
                                                                                                           tGATATATCGGGCCTCAATACGACATCTTTATCTAAATATACCTGTCTGATCTGATCCAGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACat              <  8:175356/139‑2 (MQ=255)
                                                                                                              tAAATCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGt            <  2:358976/138‑1 (MQ=255)
                                                                                                               aaaTCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGGCCGCCTGTTACGTCCATTTCCTGCATACATGTTa          <  2:361199/139‑1 (MQ=255)
                                                                                                                aaTCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTa          <  3:325250/138‑1 (MQ=255)
                                                                                                                aaTCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTAc         <  1:304816/139‑1 (MQ=255)
                                                                                                                  tCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCg       <  4:359204/139‑1 (MQ=255)
                                                                                                                  tCGGGCCTCAATACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCg       <  3:428782/139‑1 (MQ=255)
                                                                                                                      gcctcaaTACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCg       <  7:425989/130‑1 (MQ=255)
                                                                                                                           aaTACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGt                                                                        >  2:22314/1‑65 (MQ=255)
                                                                                                                           aaTACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGt                                                                        <  1:22314/65‑1 (MQ=255)
                                                                                                                                   ||                                                                                                                             
TCTCTGCTACCCTATTCTCTAACTCAACTCATTC‑AATCGCTTTATACACACTCTTGTGTATTTCTAAGCTGTTCCTATCGTATTACAATTCAGTATTTTTTGTCAATGATAAATCGGGTCTTAATACAACGGCTTTTTCTAAATATACATGCCTGATCCGTTCCTGAGGAACATCTGTCTGGACGGTCATCATGACTCTGATGCACTTTTTCAGACCGCCGGTTACGTCCATTTCCTGCATACAGGTTACCGGCACA  >  CP006881/2195415‑2195671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑