| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,198,604 | 2 bp→AA | coding (378‑379/771 nt) | U712_11015 ← | Chemotaxis protein methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,198,604 | 0 | G | A | 98.0% | 145.7 / ‑4.5 | 49 | L127L (CTG→TTA) | U712_11015 | Chemotaxis protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (21/27); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
| * | CP006881 | 2,198,605 | 0 | G | A | 97.9% | 142.3 / ‑4.4 | 49 | I126I (ATC→ATT) | U712_11015 | Chemotaxis protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (21/26); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01 | |||||||||||
CATAACTTCTATTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCTGATAGACGCCTTTTTTCGCTTTCTCCAATGCTTTTTCATCAATATCAGTCGCCAGGATCTGAAAGCCGGGAAGACTTTTTTGCTGATCCAAAAGCATGGCAAGCGTGTAAGGCTCCTCACCCGTTGAGCAGGCGGCACTCCATATCTTTAAAGGCTTACCGTTCTTGATTAACGGCAG > CP006881/2198475‑2198727 || cATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGa < 3:331592/137‑1 (MQ=255) aTAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAg < 7:254042/139‑1 (MQ=255) aTAACTTCTATTTGCGTTATGCGTAATATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAATACCGCTCCTGATAATCGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAg < 2:365270/139‑1 (MQ=255) cTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGata < 4:315547/131‑3 (MQ=255) cTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGata > 3:315547/1‑129 (MQ=255) tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa < 1:347301/136‑1 (MQ=255) tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa < 5:380305/136‑1 (MQ=255) tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa < 5:68061/136‑1 (MQ=255) tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa < 6:19195/136‑1 (MQ=255) tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa < 3:296291/136‑1 (MQ=255) tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGAc > 2:51100/1‑139 (MQ=255) gcgttttGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGAcct < 4:98203/135‑3 (MQ=255) gttttGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACctttt > 7:140764/2‑139 (MQ=255) ttgcgaaaaTAACGATCATTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTATCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGc < 4:276149/133‑1 (MQ=255) gcgTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCt < 7:4063/136‑1 (MQ=255) gcgTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCt < 8:140764/136‑1 (MQ=255) cGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCa > 7:123313/1‑139 (MQ=255) cGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCa > 2:249941/1‑139 (MQ=255) aTCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATg > 5:150741/1‑139 (MQ=255) tttCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGATGACCTTTTTGCTGGTCCAAAAGCATGGc > 6:413373/2‑138 (MQ=255) tttCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGc > 4:438552/2‑138 (MQ=255) ttCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGc > 4:128305/1‑137 (MQ=255) tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATgg < 2:321569/135‑1 (MQ=255) tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa < 7:169309/138‑1 (MQ=255) tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa < 4:60538/138‑1 (MQ=255) tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa < 4:65381/138‑1 (MQ=255) tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCAGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa < 3:72123/138‑1 (MQ=255) cTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGctcctc < 7:372215/139‑1 (MQ=255) ttCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGctcctcc > 5:181863/1‑138 (MQ=255) ccTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTcccc > 5:389967/1‑139 (MQ=255) gACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGc > 3:112376/1‑139 (MQ=255) aCCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGACTCCTCCCCCGTTgcg > 2:184476/1‑135 (MQ=255) cgctcCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAgg > 7:303128/3‑139 (MQ=255) cGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCa < 8:212700/95‑1 (MQ=255) cGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCa > 7:212700/1‑95 (MQ=255) cGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCaaat > 6:355671/1‑139 (MQ=255) cGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCaaat > 2:283264/1‑139 (MQ=255) ccTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAATCAAGGCAAGCGTGTATGGCTCCTCCCCCGTTGCGCAGGCGGCCCTCCaaatat > 2:441274/1‑137 (MQ=255) ccTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCaaattt > 6:223418/1‑137 (MQ=255) ttttCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTa < 6:150741/138‑1 (MQ=255) ttttCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTa < 6:389967/138‑1 (MQ=255) tttCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTaa < 1:441274/138‑1 (MQ=255) ttttcCAATGCTTTTTCATCAATATCCGTCGCTAATATCAGATAGCCCGGAAGACCTTTTTGCGGGTCCAATAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTAAAGGCCTag < 8:314438/136‑2 (MQ=255) tttcCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTAAAGGCCTag < 8:428353/136‑2 (MQ=255) ttcCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGctc > 2:428877/2‑93 (MQ=255) ttcCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGctc < 1:428877/92‑1 (MQ=255) tttCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCAGGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTAAAGGCCTAGAGGTCTTGAtt < 4:112376/137‑1 (MQ=255) aaTATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACGCCAAATTTTTTAAGGCCTAGAGGTCTTGATTAACGGCAg > 4:1523/1‑138 (MQ=255) aaTATCAGTCGCCAGGATCTGAAAGCCGGGAAGACTTTTTTGCTGATCCAAAAGCATGGCAAGCGTGTAAGGCTCCTCACCCGTTGAGCAGGCGGCACTCCATATCTTTAAAGGCTTACCGTTCTTGATTAACGGCAg > 6:427556/1‑138 (MQ=255) || CATAACTTCTATTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCTGATAGACGCCTTTTTTCGCTTTCTCCAATGCTTTTTCATCAATATCAGTCGCCAGGATCTGAAAGCCGGGAAGACTTTTTTGCTGATCCAAAAGCATGGCAAGCGTGTAAGGCTCCTCACCCGTTGAGCAGGCGGCACTCCATATCTTTAAAGGCTTACCGTTCTTGATTAACGGCAG > CP006881/2198475‑2198727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |