| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,174,146 | 2 bp→GC | coding (779‑780/1194 nt) | U712_10875 ← | CCA‑adding enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,174,146 | 0 | T | G | 94.4% | 39.7 / NA | 18 | E260G (GAA→GGC) | U712_10875 | CCA‑adding enzyme |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (10/7); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | CP006881 | 2,174,147 | 0 | T | C | 94.4% | 47.8 / NA | 18 | E260G (GAA→GGC) | U712_10875 | CCA‑adding enzyme |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (10/7); minor base A (1/0); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATCGCTTCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGATCAATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAAAACGGAAACTCACTCGCCGCAAGCAAGTTCTCTCGTTTATGATAAAAACCAGGGAGTTCCTCGTACAGCCCC > CP006881/2174015‑2174272 || ttcccAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATAGTCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAgg < 4:179756/136‑2 (MQ=255) agCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAAt > 3:386818/2‑138 (MQ=255) tGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGttaattaa > 4:439254/1‑136 (MQ=255) tACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAgag < 6:350233/139‑3 (MQ=255) ttgcgtCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAg > 7:166910/5‑139 (MQ=255) ttgcgtCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTAAAAAATATTACAAGGTTAATTAAAAGAGCAGCCCaaac > 2:359990/5‑138 (MQ=255) gcgtCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGttaattaa < 2:104026/120‑1 (MQ=255) gcgtCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGttaattaa > 1:104026/3‑122 (MQ=255) aaGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTGTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCa > 5:100129/1‑139 (MQ=255) tgtgAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAagagaag < 2:116458/131‑4 (MQ=255) tgtgAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTGCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAagagaag > 1:116458/1‑128 (MQ=255) tccttgATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTTAAAGATCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTcgtgg > 3:241331/1‑136 (MQ=255) ccttgATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTcgttg > 4:298788/1‑135 (MQ=255) aGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTAt > 1:66109/1‑139 (MQ=255) tCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAg > 8:34147/1‑138 (MQ=255) ggAAAAGGTGCGCGTCTTTCAAAACTATGCAATGGTTAATTTAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGCTTTCTCGTTTTTGATAAAAACCAggaat < 6:393025/139‑4 (MQ=255) aaGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCTAAGCTCTTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTct < 4:34442/139‑2 (MQ=255) tCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACaatctc < 6:407125/137‑6 (MQ=255) || TCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATCGCTTCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGATCAATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAAAACGGAAACTCACTCGCCGCAAGCAAGTTCTCTCGTTTATGATAAAAACCAGGGAGTTCCTCGTACAGCCCC > CP006881/2174015‑2174272 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |