Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,175,004	2 bp→CA	coding (1059‑1060/1134 nt)	U712_10880 ←	putative glycosyltransferase ypjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,175,004	0	T	C	100.0%	193.5 / NA	59	K354E (AAA→GAA)	U712_10880	putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/27);  total (32/27)
*	CP006881	2,175,005	0	G	A	100.0%	185.3 / NA	59	L353L (CTC→CTT)	U712_10880	putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/27);  total (32/27)

CTTCGATTAAGGTGCGGAGCACGGGAAGTGCTTTGATAAAAACTTGTTCCATATTATCACTCCGGTTCTGCTAAATCGGCATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCTAAAATACTCATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAAT  >  CP006881/2174874‑2175133                                                                                                                                   ||                                                                                                                                 cTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCAtct                                                                                                                           <  8:259509/139‑1 (MQ=255)      aTTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTAtt                                                                                                                      <  4:45029/139‑1 (MQ=255)           gatGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGc                                                                                                                 <  4:325403/137‑1 (MQ=255)               cGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCcttt                                                                                                             >  6:81322/1‑137 (MQ=255)                ggAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTg                                                                                                            >  7:206762/1‑139 (MQ=255)                    cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                                                                                                         >  4:168207/1‑138 (MQ=255)                    cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                                                                                                         >  5:198891/1‑138 (MQ=255)                    cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTACTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                                                                                                         >  5:355103/1‑138 (MQ=255)                        ggAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACga                                                                                                    <  1:48304/139‑2 (MQ=255)                        ggAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACga                                                                                                    <  5:81322/139‑2 (MQ=255)                            gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc                                                                                                >  5:183583/1‑139 (MQ=255)                            gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc                                                                                                >  4:165853/1‑139 (MQ=255)                            gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc                                                                                                >  8:373943/1‑139 (MQ=255)                             tGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct                                                                                               <  1:186532/139‑1 (MQ=255)                                 ttGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct                                                                                               <  5:436952/135‑1 (MQ=255)                                 ttGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct                                                                                               >  6:436952/1‑135 (MQ=255)                                  tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTTCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAg                                                                                            <  6:355103/137‑1 (MQ=255)                                  tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAg                                                                                            <  6:198891/137‑1 (MQ=255)                                  tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCt                                                                                          >  6:217022/1‑139 (MQ=255)                                      aaaaaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTc                                                                                      <  3:24222/139‑1 (MQ=255)                                         aaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtc                                                                                   <  5:217022/139‑1 (MQ=255)                                         aaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtc                                                                                   <  8:364586/139‑1 (MQ=255)                                              tttCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtcttct                                                                               >  4:189394/2‑138 (MQ=255)                                              tttCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtcttct                                                                               >  1:99172/2‑138 (MQ=255)                                              tttCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtcttct                                                                               >  8:361179/2‑138 (MQ=255)                                                            cTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATcgcg                                                                >  6:276055/1‑139 (MQ=255)                                                                ggTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCggg                                                            <  4:198228/139‑2 (MQ=255)                                                                    cTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGt                                                        >  2:386335/1‑139 (MQ=255)                                                                       cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTg                                                      <  2:295207/138‑1 (MQ=255)                                                                       cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGc                                                     >  5:138452/1‑139 (MQ=255)                                                                       cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGc                                                     >  2:151884/1‑139 (MQ=255)                                                                       cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGc                                                     >  2:347496/1‑139 (MQ=255)                                                                       cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCGAAAATGCTCATAGCGCGGGCTGTTGc                                                     >  8:380593/1‑139 (MQ=255)                                                                            tCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGccg                                                <  7:373943/139‑3 (MQ=255)                                                                            tCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGccg                                                <  3:165853/139‑3 (MQ=255)                                                                                 aTAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTGCATCTTCTAAACTGCTCATCGCGCGGGCTGTTGCGGCCGTAAca                                           <  8:245739/139‑2 (MQ=255)                                                                                          ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTg                                                      <  4:2666/119‑1 (MQ=255)                                                                                          ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTg                                                      >  3:2666/1‑119 (MQ=255)                                                                                          ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                  >  5:140729/1‑139 (MQ=255)                                                                                          ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                  >  3:350105/1‑139 (MQ=255)                                                                                          ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                  >  5:143029/1‑139 (MQ=255)                                                                                          ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATATTCTAAAATGCTAATCGCGCGGGCTGTTGCGGCCGTAACATCACCGAc                                   >  8:198371/1‑138 (MQ=255)                                                                                               aCTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATcgcg                                                                >  1:227237/1‑104 (MQ=255)                                                                                               aCTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATcgcg                                                                <  2:227237/104‑1 (MQ=255)                                                                                               aCTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccac                             >  3:393111/1‑139 (MQ=255)                                                                                                  ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca                           >  5:160639/1‑138 (MQ=255)                                                                                                  ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca                           >  2:196375/1‑138 (MQ=255)                                                                                                  ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca                           >  7:244255/1‑138 (MQ=255)                                                                                                    cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCaaa                         >  5:15404/1‑138 (MQ=255)                                                                                                    cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCaaa                         >  6:354358/1‑138 (MQ=255)                                                                                                    cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGATCATCGCGCGGGCTGTTGCGGCCGTACCATCACCGACATCCCCCaaa                         >  8:45242/1‑138 (MQ=255)                                                                                                       aCAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATc                     <  6:140729/139‑1 (MQ=255)                                                                                                           ttttttTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCt                 <  4:50622/139‑1 (MQ=255)                                                                                                                         aattCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTttttta   <  4:393111/136‑3 (MQ=255)                                                                                                                          attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat  <  7:361179/137‑1 (MQ=255)                                                                                                                          attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat  <  6:183583/137‑1 (MQ=255)                                                                                                                          attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat  <  3:189394/137‑1 (MQ=255)                                                                                                                          attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat  <  1:386335/137‑1 (MQ=255)                                                                                                                          attCATTTTCAAGCATCTCTAATGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat  <  5:50796/137‑1 (MQ=255)                                                                                                                                   ||                                                                                                                                 CTTCGATTAAGGTGCGGAGCACGGGAAGTGCTTTGATAAAAACTTGTTCCATATTATCACTCCGGTTCTGCTAAATCGGCATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCTAAAATACTCATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAAT  >  CP006881/2174874‑2175133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑