Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,180,739	2 bp→AA	coding (108‑109/594 nt)	U712_10915 ←	Uncharacterized protein ypjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,180,739	0	G	A	100.0%	170.4 / NA	55	L37L (CTG→TTG)	U712_10915	Uncharacterized protein ypjA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/30);  total (25/30)
*	CP006881	2,180,740	0	T	A	100.0%	164.0 / NA	55	L36L (CTA→CTT)	U712_10915	Uncharacterized protein ypjA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (25/30);  total (25/30)

GGTGACTAGCGCAAGCGCCTCAAAGAGCGGAGCGTTCCGTTTCATGATAAAGGCGAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTATCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAGTAGCTGCGGCAAGTACCAATAGTATCCGTATACCGTTCCCAGAAAATTGATGGCTAAGACAAGAATAAGCATCGGCTGCCGTCCTAATACATATTGAAACCATTTCACATCAAATCAACTCTCAGTCTGTAAA  >  CP006881/2180608‑2180872                                                                                                                                    ||                                                                                                                                     ggTAACTAGCGCAAGCGCCTCTAAAAGCGGAGCATTCCGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGc                                                                                                                                >  6:435297/1‑139 (MQ=255) ggTAACTAGCGCAAGCGCCTCTAAAAGCGGAGCATTCCGATTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCCGTGTCTCCAAAAGCTGc                                                                                                                                >  5:208357/1‑139 (MQ=255)   taaCTAGCGCAAGCGCCTCTAAAAGCGGAGCATTCCGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGCCTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCgg                                                                                                                              >  5:221574/3‑139 (MQ=255)   taaCTAGCGCAAGCGCCTCTAAAAGCGGAGCATTCCGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCgg                                                                                                                              >  7:443029/3‑139 (MQ=255)   taaCTAGCGCAAGCGCCTCTAAAAGCGGAGCATTCCGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCgg                                                                                                                              >  6:197631/3‑139 (MQ=255)    aaCTAGCGCACGCGCCTCTAAAAGCGGAGCATTCCGTTTCATGAGAAAGGCAAGCAGCACAAACACAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCgg                                                                                                                              <  6:207334/137‑1 (MQ=255)     aCTAGCGCAAGCGCCTCTAAAAGCGGAGCATTCCGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGc                                                                                                                             >  4:317391/1‑138 (MQ=255)     aCTAGCGCAAGCGCCTCTAAAAGCGGAGCATTCCGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCa                                                                                                                            <  8:443029/139‑1 (MQ=255)      ctcgcgcAAGCGCCTCTAAAAGCGGAGCATTCCGTTTCATGAGAAAGGCAAGCACCACAAACACAAACAAAAACGTGGCTGTCGGACTGGCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCaa                                                                                                                           <  1:277682/136‑1 (MQ=255)          cgcAAGCGCCTCTAAAAGCGGAGCATTCCGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCTGCAAGTAc                                                                                                                       >  3:248845/1‑139 (MQ=255)            cAAGCGCCTCTAAAAGCGGAGCATTCCGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCTAAAGCTGCGGCAAGTAcc                                                                                                                      >  5:6517/1‑138 (MQ=255)            cAAGCGCCTCTAAAAGCGGAGCATTCCGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTAcc                                                                                                                      >  4:306058/1‑138 (MQ=255)            cAAGCGCCTCTAAAAGCGGAGCATTCAGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTAcc                                                                                                                      >  5:20280/1‑138 (MQ=255)              aGCGCCTCTAAAAGCGGAGCATTCCGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCaa                                                                                                                    >  3:169896/1‑138 (MQ=255)                cgcCTCTAAAAGCGGAGCATGCCGTTTCAGGAGAAAGGCAAGCAGCACAAACACAAACAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAg                                                                                                                 <  4:359684/139‑1 (MQ=255)                cgcCTCTAAAACCGGAGCATTCCGTTTCATGAGAAAGGCAAGCCGCACAAACAGAAAGAAAACCGTTGCTGTCGGACTGACCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAg                                                                                                                 <  3:116011/139‑1 (MQ=255)                 gcCTCTAAACGCGGAGCATCCCGTTACATCACAAAGCCAACCCCCACAACAACAAACAACAACGGTGCGGGCGGACTGCCCGGAAAAAAAAGCCGCAACCGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGt                                                                                                                <  1:176275/139‑1 (MQ=255)                       aaaagcggagcaTTCCGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGt                                                                                                          <  4:169896/136‑1 (MQ=255)                                    tCCGTTTCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTcc                                                                                                >  3:63525/1‑136 (MQ=255)                                        tttCATGAGAAAGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGaaaa                                                                                         >  7:233786/1‑139 (MQ=255)                                                 aaaGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGc                                                                                 >  2:95195/1‑138 (MQ=255)                                                      caagcagcACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGCCTAAGa                                                                            >  3:46134/3‑138 (MQ=255)                                                          cagcaCAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAg                                                                        >  1:98212/1‑138 (MQ=255)                                                                  cctaaaagaatAAGGTGGGTGGCGGACTGTCCGGAACAAAAAGACGGAAACGGGCCGGTGTCTCCAAATGCTGCGGCAAGTACAAATAGTAGCCGTAAACCGTTCCCAGAAATTTAATGGCTAAGACAAGAATAAGCAt                                                               <  2:293988/135‑1 (MQ=255)                                                                       aataaaacCGTTGCTGTCGCACTGGCCGTAACAAAAATCTGCAACCGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGcc                                                          <  4:112741/136‑2 (MQ=255)                                                                       aagaaaAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGcc                                                          >  1:406238/1‑138 (MQ=255)                                                                         taaaacCGTTGCTGGCGGCCTGTCCGCAACAAAAAGCAGGAACCGGGCCGGTGGCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                        <  8:440211/138‑1 (MQ=255)                                                                         gaaaAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                        <  7:153073/139‑1 (MQ=255)                                                                         caaacccGTGGCGGTGGGACTGTCCGGAAAAAAAAGCAGCCAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                        <  7:183128/133‑1 (MQ=255)                                                                         caaaaaCTTTGCGGGCGTCCTGGCCGTAAAAAAAAGCCGCACACGGGCCGGGGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                        <  3:43811/138‑1 (MQ=255)                                                                         caaaaaCGTTGCTGTGGGACTGTCCGGAACAAAAATCACGAACCGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCTATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                        <  3:163374/138‑1 (MQ=255)                                                                                   gCTGGCGGACGGTCCGGAACAAAAATCAGGAACCGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATa                                              <  4:182062/139‑1 (MQ=255)                                                                                    cTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATa                                              >  5:123287/1‑138 (MQ=255)                                                                                    cTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATa                                              <  5:197631/138‑1 (MQ=255)                                                                                           aCTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGa                                      <  3:317391/139‑1 (MQ=255)                                                                                            cttaccGAAACAAAAAACAGCAAACGGGCCTGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTA‑ACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGaaa                                    <  5:435297/135‑1 (MQ=255)                                                                                               tCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAAtta                                                                                      >  4:390256/1‑86 (MQ=255)                                                                                               tCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAAtta                                                                                      <  3:390256/87‑2 (MQ=255)                                                                                               tCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATGAGCATCGGCCGCTGCCCTAATACATATTGAAAc                                   >  7:189548/1‑138 (MQ=255)                                                                                                 cGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTATCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCAt                                >  3:384012/1‑139 (MQ=255)                                                                                                  ggAAAAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCAtt                               <  4:367734/139‑1 (MQ=255)                                                                                                   taaCAAAAATCTGCAACCGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCTTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCAttt                              <  7:52047/138‑1 (MQ=255)                                                                                                    aaCAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCAttt                              >  1:4007/1‑138 (MQ=255)                                                                                                        aaacccAGTAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCAGTAAACCGTTCCCATAATATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACat                         <  8:233786/134‑1 (MQ=255)                                                                                                        aaacTCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACat                         <  1:95195/139‑1 (MQ=255)                                                                                                         aaaTCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGa                                                                            <  1:100580/87‑1 (MQ=255)                                                                                                         aaaTCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGa                                                                            >  2:100580/1‑87 (MQ=255)                                                                                                         aaaTCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                        <  6:223284/107‑1 (MQ=255)                                                                                                         aaaTCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                        >  5:223284/1‑107 (MQ=255)                                                                                                         aaaTCAGGAAACGGGCCAGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                        >  1:54769/1‑107 (MQ=255)                                                                                                         aaaTCAGGAAACGGGCCAGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                        <  2:54769/107‑1 (MQ=255)                                                                                                                         cGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTc        <  4:180053/139‑1 (MQ=255)                                                                                                                         cGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTc        <  6:123287/139‑1 (MQ=255)                                                                                                                             gtCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTa    <  7:171985/139‑1 (MQ=255)                                                                                                                               ctcCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTaaa  <  5:187568/139‑1 (MQ=255)                                                                                                                                    ||                                                                                                                                     GGTGACTAGCGCAAGCGCCTCAAAGAGCGGAGCGTTCCGTTTCATGATAAAGGCGAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTATCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAGTAGCTGCGGCAAGTACCAATAGTATCCGTATACCGTTCCCAGAAAATTGATGGCTAAGACAAGAATAAGCATCGGCTGCCGTCCTAATACATATTGAAACCATTTCACATCAAATCAACTCTCAGTCTGTAAA  >  CP006881/2180608‑2180872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑