Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,185,690	2 bp→GC	coding (101‑102/180 nt)	U712_10950 ←	Hypothetical Protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,185,690	0	T	G	100.0%	104.2 / NA	33	K34S (AAA→AGC)	U712_10950	Hypothetical Protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/16);  total (17/16)
*	CP006881	2,185,691	0	T	C	100.0%	106.4 / NA	33	K34S (AAA→AGC)	U712_10950	Hypothetical Protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/16);  total (17/16)

AAAGCCCCCGGCAAATAAGATGCTTGCTGTACGGCAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGTTCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAACAGCCCTCTGACCTGCCGTTTTGTA‑‑TAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTC  >  CP006881/2185553‑2185776                                                                                                                                          ||                                                                                        aaaGCCCCCGGCAAATAAGATGCTTGCTGTACGGCAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGTTCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAACAGCCCTCTGACCTGCCGtt                                                                                         <  6:101749/139‑1 (MQ=255)     cccccGGCAAATAAGATGCTT‑CTGTACGGAAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGt                                                                                     <  5:72504/138‑1 (MQ=255)       caccgCAAATAAGATGCTT‑CTGGACGGAAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGtaag                                                                                  <  6:609/135‑3 (MQ=255)        ccGGCAAATAAGATGCTT‑CTGTACGGAAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGtaag                                                                                  >  2:134920/1‑136 (MQ=255)         cGGCAAATAAGATGCTT‑CTGTACGGAAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGtaagta                                                                                <  4:303727/139‑5 (MQ=255)          ggCAAATAAGATGCTT‑CTGTACGGAAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGt                                                                                     <  8:168507/133‑1 (MQ=255)          ggCAAATAAGATGCTT‑CTGTACGGAAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGt                                                                                     >  7:168507/1‑133 (MQ=255)           gCAAATAAGATGCTT‑CTGTACGGAAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGtaagtaaa                                                                              <  2:35055/139‑7 (MQ=255)                   gATGCTT‑CTGTACGGAATGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATcc                                                                      <  7:175680/139‑1 (MQ=255)                    aTGCTT‑CTGTACGGAAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATccc                                                                     >  8:309738/1‑139 (MQ=255)                        cttctGTACGGAAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATcccccc                                                                  >  8:202496/4‑139 (MQ=255)                          tctGTACGGAAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCtt                                                                >  8:366414/2‑139 (MQ=255)                                   aaGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCGttttt                                                       >  8:388446/2‑139 (MQ=255)                                   aaGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCGttttt                                                       >  5:83651/2‑139 (MQ=255)                                   aaGGGAATTATTGAGTCTTATTAGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCGttttt                                                       >  4:253231/2‑139 (MQ=255)                                                      attaGTTTACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGa                                     <  6:83651/137‑1 (MQ=255)                                                            ttACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTa                              <  5:80400/138‑1 (MQ=255)                                                            ttACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTa                              >  6:80400/1‑138 (MQ=255)                                                            ttACCAAAAGATCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTAt                             >  3:157767/1‑139 (MQ=255)                                                                     gatCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGttttttcg                    >  2:392884/3‑137 (MQ=255)                                                                     gatCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGttttttcg                    >  5:337110/3‑137 (MQ=255)                                                                        cTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATACGTTCTATAAGACAAGCTATGttttttctcct                 >  3:413169/1‑134 (MQ=255)                                                                           gagaTAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGcc                  <  5:291619/135‑1 (MQ=255)                                                                           gagaTAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGcc                  <  7:309738/135‑1 (MQ=255)                                                                           gagaTAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGcc                  <  7:202496/135‑1 (MQ=255)                                                                           gagaTAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGcc                  <  7:140423/135‑1 (MQ=255)                                                                                aGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGCCTCGAATTAt         >  4:318150/1‑139 (MQ=255)                                                                                        aaaaaaGGAAGATCGCCCTCTGTACAGTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGCCTCGAATTATGAtctgc  <  1:392884/138‑5 (MQ=255)                                                                                                                  gTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGcc                  <  2:45223/96‑1 (MQ=255)                                                                                                                  gTAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGcc                  >  1:45223/1‑96 (MQ=255)                                                                                                                   tAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGa                                     <  3:193921/76‑1 (MQ=255)                                                                                                                   tAAAGCAGCTCTTTGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGa                                     >  4:193921/1‑76 (MQ=255)                                                                                                                             ctttGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGCCTCGAATTATGAt      >  3:172057/4‑97 (MQ=255)                                                                                                                             ctttGACCTGCCGGCTTGTAAGTAAAGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGCCTCGAATTATGAt      <  4:172057/94‑1 (MQ=255)                                                                                                                                          ||                                                                                        AAAGCCCCCGGCAAATAAGATGCTTGCTGTACGGCAGGGAATTATTGAGTCTTATTAGTTTACCAAAAGTTCTTGAGATAGAAAATGAAAAAAGGAAGATCGCCCTCTGTACAGTAAAACAGCCCTCTGACCTGCCGTTTTGTA‑‑TAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTC  >  CP006881/2185553‑2185776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑