Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,186,864 2 bp→TT coding (236‑237/1287 nt) U712_10955 ← 3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,186,8640AT98.8% 256.8 / NA 83S79K (AGT→AAA) U712_109553‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (46/36);  total (46/37)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0068812,186,8650CT98.8% 258.4 / NA 83S79K (AGT→AAA) U712_109553‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (45/37);  minor base G (1/0);  total (46/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTTCATTGGGCGCTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAAAATGCCGAGCATGAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCACTCAGGGAATCGATTCCATTTCCGTGAATCACTACATTGCTGCCGTTTTGCTCAATTTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCCGCTCCCGGCAGAAAGTTTTTCACAGTTG  >  CP006881/2186733‑2186994
                                                                                                                                   ||                                                                                                                                 
cgcttCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTTAAAAGGACGGCCCGCCATTATTCCGAGCATCAGGCGATTCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAggg                                                                                                                             >  2:392831/4‑139 (MQ=255)
     cATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATc                                                                                                                         >  2:194218/1‑138 (MQ=255)
      aTTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGa                                                                                                                       <  1:75456/139‑1 (MQ=255)
      aTTGGGCGTTTCGCAATGCTATCATCTCCGGCAACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGa                                                                                                                       <  5:22350/139‑1 (MQ=255)
         gggCGTTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTc                                                                                                                    >  8:2929/1‑139 (MQ=255)
                      tGCTCCCATCTCCGGCTACCGCGCGGTAAAAAGCACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAAt                                                                                                       <  4:429831/139‑1 (MQ=255)
                      agacagcATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCTCCAATATTCCGAGCAGCAGGCGTATCGTTGTACCTGAATTTCCGACAACTAAAAGGCTTTCTGGCTCTTTCAGGGCAGCGATTCCTTTTCCGTGAAt                                                                                                       <  6:166955/133‑1 (MQ=255)
                           tcATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCAcga                                                                                                  >  2:18442/1‑137 (MQ=255)
                             aTCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGAc                                                                                                 >  5:276678/1‑138 (MQ=255)
                             aTCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACa                                                                                                >  5:189388/1‑139 (MQ=255)
                                tcCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATc                                                                                                                         <  8:280342/111‑1 (MQ=255)
                                tcCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATc                                                                                                                         >  7:280342/1‑111 (MQ=255)
                                  cGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTTTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgct                                                                                           >  2:82164/1‑139 (MQ=255)
                                  cGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGAc                                                                                                 >  7:253044/1‑133 (MQ=255)
                                  cGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGAc                                                                                                 <  8:253044/133‑1 (MQ=255)
                                  cGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgct                                                                                           >  7:266131/1‑139 (MQ=255)
                                   ggCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTTTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgctg                                                                                          <  1:82164/139‑1 (MQ=255)
                                   ggCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgctg                                                                                          <  1:18442/139‑1 (MQ=255)
                                   ggCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgctg                                                                                          <  7:191969/139‑1 (MQ=255)
                                   ggCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgctg                                                                                          <  7:50440/139‑1 (MQ=255)
                                   ggCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgctg                                                                                          <  7:348305/139‑1 (MQ=255)
                                   ggCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgcag                                                                                          >  7:115960/1‑137 (MQ=255)
                                       aCCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgctgctgc                                                                                      <  3:283721/139‑4 (MQ=255)
                                       aCCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCAGACATCTAAAAGGCTTTCTGGCTCTTTCAGTGCATCGATTCCTTTTCCGTGAATCACTACATagcctgtc                                                                                       >  5:166955/1‑133 (MQ=255)
                                        ccGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgctgctgct                                                                                     <  6:169035/139‑5 (MQ=255)
                                        ccGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgctgctgct                                                                                     <  6:38322/139‑5 (MQ=255)
                                         cgcgCTGTAAAAAGGCCGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTGCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAAGCGCGACATcggtgctggtt                                                                                    >  2:389875/1‑134 (MQ=255)
                                           cgcTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAg                                                                                                                               >  3:390201/1‑94 (MQ=255)
                                           cgcTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAg                                                                                                                               <  4:390201/94‑1 (MQ=255)
                                             cTGTACAAAGGACGGCCCGACAATATTCCGAGCATCAGTCGAATCCTTGTACCTGAATTTCCGACATCTAACAGGCGTTCTGGCTCTTTCAGGGCATCGCTTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCt                                                                                <  2:44219/139‑1 (MQ=255)
                                                tAAAACGGCCGGCCCGCCAATAATCCGAGCATCAGGCGAATCGTAGTACCTGAATTTCAGACATCTAAAAGGCTTTCTGGCTCATTCAGGGCCTCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTc                                                                               <  1:392831/137‑1 (MQ=255)
                                                tAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCGTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCaa                                                                             <  7:226707/139‑1 (MQ=255)
                                                tAAAAAGGAAGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCTACATCTAAAAGGCTTTCTGGCTCTTTCAGTGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTc                                                                               <  8:7151/137‑1 (MQ=255)
                                                     aGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAAtgtg                                                                         >  6:336619/1‑138 (MQ=255)
                                                      ggACGGCCCGCGAATAGTCCGAGCAGCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGGATCGATTCCTTTTCCGGTAATCACGACATCGCTGCTGCGTTGCTCAATGTGaa                                                                       <  4:52958/139‑1 (MQ=255)
                                                          ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGCCATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAcc                                                                   >  6:153261/1‑139 (MQ=255)
                                                          ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCg                                                                                                            <  6:36921/98‑1 (MQ=255)
                                                          ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCg                                                                                                            >  5:36921/1‑98 (MQ=255)
                                                          ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgctg                                                                                          <  1:429400/116‑1 (MQ=255)
                                                          ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgctg                                                                                          >  2:429400/1‑116 (MQ=255)
                                                          ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCTATGTGAACAcc                                                                   >  8:445822/1‑139 (MQ=255)
                                                          ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAcc                                                                   >  1:122822/1‑139 (MQ=255)
                                                          ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAcc                                                                   >  7:182493/1‑139 (MQ=255)
                                                          ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAcc                                                                   >  2:5478/1‑139 (MQ=255)
                                                          ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAcc                                                                   >  7:259418/1‑139 (MQ=255)
                                                          ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAcc                                                                   >  7:305091/1‑139 (MQ=255)
                                                          ggCCCGCCAATATTCCGAGCATCAGGCGAATAGTTGTACATGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGGACAcc                                                                   >  5:173554/1‑139 (MQ=255)
                                                                 cAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCAtttt                                                             >  2:138213/1‑138 (MQ=255)
                                                                    tattccGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTa                                                         <  5:336619/135‑1 (MQ=255)
                                                                      ttccGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTa                                                         >  8:121982/3‑137 (MQ=255)
                                                                           aGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgctgctgct                                                                                     >  4:240744/1‑100 (MQ=255)
                                                                           aGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATcgctgctgct                                                                                     <  3:240744/104‑5 (MQ=255)
                                                                           aGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAat                                                  <  6:189388/139‑1 (MQ=255)
                                                                           aGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAat                                                  >  7:108449/1‑139 (MQ=255)
                                                                           aGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAACCATTTTTCTAAAGCAat                                                  >  4:295379/1‑139 (MQ=255)
                                                                           aGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTGTCAGGGCATCGATTCGTTTTCCGTGAATCACGACATCGATGATGCTTTGCTCAATGTGAACACCGTTTTTTCTAAAGAAat                                                  >  4:293198/1‑139 (MQ=255)
                                                                               tcaGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAatcgat                                              <  4:130236/137‑1 (MQ=255)
                                                                               tcaGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCAGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAatcgat                                              <  6:276678/137‑1 (MQ=255)
                                                                                caGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAatcgatc                                             >  4:20245/2‑139 (MQ=255)
                                                                                caGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAatcgatc                                             >  5:346565/2‑139 (MQ=255)
                                                                                      aaTCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTc                                       <  7:121982/139‑1 (MQ=255)
                                                                                            tGTACCTGAATTTACGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTAGCTCAATGTGAACACCCATTTATCTAAAGCAAACGATCGTGCTCAGACa                                  >  3:157610/1‑138 (MQ=255)
                                                                                                    aaTTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTc                          <  1:138213/138‑1 (MQ=255)
                                                                                                      tttCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCg                       >  7:158916/1‑139 (MQ=255)
                                                                                                        tCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTaa                                                        >  4:242509/1‑104 (MQ=255)
                                                                                                        tCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTaa                                                        <  3:242509/104‑1 (MQ=255)
                                                                                                        tCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCgg                      >  7:211433/1‑138 (MQ=255)
                                                                                                        tCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCgg                      >  4:112831/1‑138 (MQ=255)
                                                                                                          cGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCgg                      >  3:37058/1‑136 (MQ=255)
                                                                                                           gACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGGACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGa                  <  6:173554/139‑1 (MQ=255)
                                                                                                           gACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGa                  <  8:211433/139‑1 (MQ=255)
                                                                                                                cTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTa                                                         >  2:376539/1‑95 (MQ=255)
                                                                                                                cTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTa                                                         <  1:376539/95‑1 (MQ=255)
                                                                                                                cTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAATGtt             >  7:47262/1‑139 (MQ=255)
                                                                                                                     aGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGtttttaa        <  8:158916/139‑3 (MQ=255)
                                                                                                                       gCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTaact      <  1:114363/139‑2 (MQ=255)
                                                                                                                          ttCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGa                  <  3:100418/124‑1 (MQ=255)
                                                                                                                          ttCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTgtt   >  4:379438/1‑139 (MQ=255)
                                                                                                                          ttCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCAATTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGa                  >  4:100418/1‑124 (MQ=255)
                                                                                                                           tCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGtttttaa        >  3:187752/1‑131 (MQ=255)
                                                                                                                           tCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGtttttaa        <  4:187752/133‑3 (MQ=255)
                                                                                                                           tCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTgttg  >  1:152939/1‑139 (MQ=255)
                                                                                                                           tCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTgttg  >  5:178919/1‑139 (MQ=255)
                                                                                                                                   ||                                                                                                                                 
CGTTTCATTGGGCGCTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAAAATGCCGAGCATGAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCACTCAGGGAATCGATTCCATTTCCGTGAATCACTACATTGCTGCCGTTTTGCTCAATTTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCCGCTCCCGGCAGAAAGTTTTTCACAGTTG  >  CP006881/2186733‑2186994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑