Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,401,656 Δ1 bp 6.3% coding (527/1044 nt) U712_07165 → putative membrane protein ykvI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,401,6560G. 6.3% 218.3 / 8.0 63coding (527/1044 nt)U712_07165putative membrane protein ykvI
Reads supporting (aligned to +/- strand):  ref base G (40/19);  new base . (2/2);  total (42/21)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

TGATGACTAGAGGAGTAAAAGGCATTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGGAGAAAGTGATAAAAAAAGGCGCTTTAATCG  >  CP006881/1401524‑1401790
                                                                                                                                    |                                                                                                                                      
tgaagaCTAGAGGAGTAAAAGGCATTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATTATTTTGGTGTCCGACTCTTTTATATTCATCGAATCACTAAACGAATCTAAGTGGGTCTGGCCGc                                                                                                                                   >  4:229953/1‑138 (MQ=255)
  atgaCTAGAGGAGTAAAAGGCATTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGAGTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATc                                                                                                                                >  5:44836/1‑139 (MQ=255)
  atgaCTAGAGGAGTAAAAGGCATTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATc                                                                                                                                >  1:28300/1‑139 (MQ=255)
  atgaCTAGAGGAGTAAAAGGCATTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATc                                                                                                                                >  7:123220/1‑139 (MQ=255)
         gaggagTAAAAGGCATTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTggg                                                                                                                          <  2:28300/138‑1 (MQ=255)
          aggagTAACAGGCATTTTCGGTGGCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGGGGGGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGAt                                                                                                                        <  8:244272/139‑1 (MQ=255)
            gagTAAAAGGCATTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCTCTAACCGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTg                                                                                                                      >  2:350087/1‑139 (MQ=255)
             agTAAAAGGCATTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTgg                                                                                                                     <  3:229953/139‑1 (MQ=255)
                 aaaGGCATTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGt                                                                                                                 >  7:246358/1‑139 (MQ=255)
                 aaaGGCATTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGCCCGCATCGAGGGGATTGGCTGt                                                                                                                 >  6:37402/1‑139 (MQ=255)
                    ggCATTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTa                                                                                                               >  6:251184/1‑138 (MQ=255)
                       aTTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATcag                                                                                                           <  4:36270/139‑1 (MQ=255)
                       aTTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATcag                                                                                                           <  8:246358/139‑1 (MQ=255)
                       aTTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTCGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGCCTGTTAt                                                                                                              >  7:116893/1‑136 (MQ=255)
                          ttCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATcagca                                                                                                         <  8:256794/138‑1 (MQ=255)
                            cGGTGTCAATGTGTGTGTTGGTCCTCTTTAAATCATCTTTTCAATGATTGGGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGGCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGt                                                                                                       <  6:83326/138‑1 (MQ=255)
                              gtgtCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTC‑‑GCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCtt                                                                                                  >  1:240638/1‑139 (MQ=255)
                              gtgtCAATGTGTATGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTC‑‑GCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCt                                                                                                   >  3:120991/1‑138 (MQ=255)
                                   aaTGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACg                                                                                               <  5:251184/139‑1 (MQ=255)
                                   aaTGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACg                                                                                               <  4:159511/139‑1 (MQ=255)
                                         tttgttGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCtt                                                                                         >  7:80180/1‑139 (MQ=255)
                                               gttCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGCCGGTTTTATATTCCGCGCATCCCGAAACGCAGGTCCGGGGGTGGGGCCGGATGCCGGGGGTTGGGTGTTTTCAGCAGTTTGGTTCGGGGGTTGaaaat                                                                                   >  2:179191/1‑137 (MQ=255)
                                                              atcTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAg                                                                    <  4:250759/139‑1 (MQ=255)
                                                                  tttCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTgg                                                                >  2:211932/1‑138 (MQ=255)
                                                                        tGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGt                                                          >  5:160453/1‑139 (MQ=255)
                                                                             gtggtgGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCg                                                      <  7:111141/138‑1 (MQ=255)
                                                                                gtggCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTTAATTTATCGCTTGCCCAAGCTGGTTTGGTTCCGCTgg                                                  >  3:234833/1‑139 (MQ=255)
                                                                                    ccGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCg                                                                                                                               >  7:177907/1‑58 (MQ=255)
                                                                                    ccGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCg                                                                                                                               <  8:177907/58‑1 (MQ=255)
                                                                                       aCTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTC‑‑GCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGaaa                                         <  4:120991/139‑1 (MQ=255)
                                                                                       aCTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTC‑‑GCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGaaa                                         <  2:240638/139‑1 (MQ=255)
                                                                                                   tCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTgg                                                                                                                     >  6:245248/1‑53 (MQ=255)
                                                                                                   tCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTgg                                                                                                                     <  5:245248/53‑1 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCt                                                                                                                             >  1:344602/1‑43 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCt                                                                                                                             <  2:344602/43‑1 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTgg                                                                                                                     <  1:239114/51‑1 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTgg                                                                                                                     >  2:239114/1‑51 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAAtt                                                                                   >  5:59483/1‑85 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAAtt                                                                                   <  6:59483/85‑1 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGc                                                                   >  1:272216/1‑101 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGc                                                                   <  2:272216/101‑1 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGATTGAAATGTCATCGgag                              >  7:322919/1‑138 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGgag                              >  5:193533/1‑138 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGgag                              >  7:67385/1‑138 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGgag                              >  3:167122/1‑138 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGgag                              >  3:189695/1‑138 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGgag                              >  4:56486/1‑138 (MQ=255)
                                                                                                     aGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCGTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGgag                              >  5:88534/1‑138 (MQ=255)
                                                                                                       cGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAAt                                        >  3:198690/1‑126 (MQ=255)
                                                                                                       cGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAAt                                        <  4:198690/126‑1 (MQ=255)
                                                                                                       cGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGgaga                             >  3:248406/1‑137 (MQ=255)
                                                                                                       cGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGgaga                             >  7:181437/1‑137 (MQ=255)
                                                                                                       cGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGgaga                             >  6:109997/1‑137 (MQ=255)
                                                                                                          aTCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGGAGAAAGt                         >  7:318116/1‑138 (MQ=255)
                                                                                                          aTCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGATGGCGAATGAAATGTCATCGGAGAAAGt                         >  1:305014/1‑138 (MQ=255)
                                                                                                           tCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGTCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGGAGAAAGTGa                       <  6:44836/139‑1 (MQ=255)
                                                                                                                     cAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGATTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGGAGAAAGTGATAAAAAAAgg             >  6:47257/1‑139 (MQ=255)
                                                                                                                           aGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTAATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGGTGGCGAATGAAATGTCATCGGAGAAAGTGATAAAAAAATGCGCtt        >  1:232760/1‑138 (MQ=255)
                                                                                                                             tGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGGAGAAAGTGATAAAAAAAGGCGCTTTaa     >  1:59943/1‑139 (MQ=255)
                                                                                                                                  cTGGCCGCATCGCTGGTATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTACGCTGGCGAATGAAATGTCATCTGAGAAAGTGATAAAAAAAGGCGCTTTAATCg  >  5:265480/1‑137 (MQ=255)
                                                                                                                                  cTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGGAGAAAGTGATAAAAAAAGGCGCTTTAATCg  >  1:77872/1‑137 (MQ=255)
                                                                                                                                  cTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGGAGAAAGTGATAAAAAAAGGCGCTTTAATCg  >  7:318085/1‑137 (MQ=255)
                                                                                                                                  cTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGGAGAAAGTGATAAAAAAAGGCGCTTTAATCg  >  3:124187/1‑137 (MQ=255)
                                                                                                                                    |                                                                                                                                      
TGATGACTAGAGGAGTAAAAGGCATTTTCGGTGTCAATGTGTTTGTTGTTCCTCTTTTAATCATCTTTTCAATGATTGTGGTGGCCGACTCTTTTATATTCAGCGAATCACGAAACGCAGCTCAGTGGGTCTGGCCGCATCGCTGGGATTGGCTGTTATCAGCAGTTTCTTACGGGGCTTTAAATTTATCGCTTGCCCAAGCTGTTTTGGTTCCGCTGGCGAATGAAATGTCATCGGAGAAAGTGATAAAAAAAGGCGCTTTAATCG  >  CP006881/1401524‑1401790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑