| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 1,655,978 | Δ1 bp | 100% | coding (430/1596 nt) | U712_08515 → | Flagellar M‑ring protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,655,978 | 0 | G | . | 98.4% | 271.7 / ‑1.9 | 61 | coding (430/1596 nt) | U712_08515 | Flagellar M‑ring protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (33/27); total (34/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAA > CP006881/1655856‑1656111 | gATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCaaaa > 5:348071/1‑139 (MQ=255)gATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCaa > 1:310047/1‑137 (MQ=255)gATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCaa > 7:39287/1‑137 (MQ=255)gATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAAATTGATCAATGAAATGGAC‑GTATTAAAAATTCaa > 7:148586/1‑137 (MQ=255) cAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGAACAATGAAATGGAC‑GTATTATAAATTCAAAAGTa < 4:104616/139‑1 (MQ=255) aaTTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGAAATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTa < 6:342626/138‑1 (MQ=255) aTTGATTATTCGTTTTTTGGACAGAATGCCGGATTTGGTTTGACAGAAAATGAGTTTGATATGGGGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAACTGCAC‑TTAGTAAAAATTCAAAAGTAAt > 4:120456/1‑139 (MQ=255) ttattCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCaa > 4:163392/1‑127 (MQ=255) ttattCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCaa < 3:163392/127‑1 (MQ=255) ttCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAAt < 5:267945/138‑1 (MQ=255) tCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAAtct > 4:166454/1‑139 (MQ=255) aTGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCtgtg < 3:156707/139‑1 (MQ=255) cGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTg > 8:38362/1‑139 (MQ=255) ggCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGt < 2:13886/139‑1 (MQ=255) gCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGt > 1:63262/1‑139 (MQ=255) aaTGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTgc < 6:348071/139‑1 (MQ=255) aaTGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTgc < 3:86649/139‑1 (MQ=255) tGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTgcag > 8:335341/1‑139 (MQ=255) tttGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCAt < 6:288449/138‑1 (MQ=255) tttGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATc > 1:326648/1‑139 (MQ=255) tGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTg > 8:117780/1‑139 (MQ=255) tGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTg > 1:176876/1‑139 (MQ=255) ggTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCaa > 5:110550/1‑139 (MQ=255) tgaaagtgaaagCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATc > 4:143057/1‑139 (MQ=255) aagtgaaagCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGtt > 4:25405/1‑139 (MQ=255) agtgaaagCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCa < 7:142616/113‑1 (MQ=255) agtgaaagCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCa > 8:142616/1‑113 (MQ=255) cacaGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCaa > 5:275189/1‑139 (MQ=255) acagacagAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAAc > 5:352179/1‑139 (MQ=255) cagacagaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAAcc < 3:25405/139‑1 (MQ=255) agacagaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCTGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCg < 1:84525/139‑1 (MQ=255) acataACTGTCAAACTTGAACAATGAAATGGAC‑GTATTAAAAATACAAAATTAATGATTAATCTCCCGAAAGACGCTTTCTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTCCAGATTCAACCggg > 2:345849/1‑139 (MQ=255) acagaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTg < 5:11523/111‑1 (MQ=255) acagaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTg > 6:11523/1‑111 (MQ=255) acagaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGtttt < 5:59426/124‑1 (MQ=255) acagaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGtttt > 6:59426/1‑124 (MQ=255) acagaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCggg > 4:34628/1‑139 (MQ=255) acagaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCggg > 8:133841/1‑139 (MQ=255) agaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCt < 5:82637/75‑1 (MQ=255) agaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCt > 6:82637/1‑75 (MQ=255) agaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGtt < 8:236412/139‑1 (MQ=255) gaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTta > 7:137742/1‑139 (MQ=255) tCAAACTTGATCAATGAAATTGAC‑GTATTAAATATTCAAAAGTAATGATTATTCTCCCTAAAGACGCTGTGTTTGTCTGTGAAGAGCAATCTGCAGCATCTGCTTCAATGGTTTTGCAGATTCAACCGGGTTATacac < 1:345849/138‑1 (MQ=255) tCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACt > 6:284324/1‑139 (MQ=255) tCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACt > 4:96110/1‑139 (MQ=255) cTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATc < 8:146105/139‑1 (MQ=255) cTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATc < 2:45927/139‑1 (MQ=255) gATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCa > 8:167165/1‑137 (MQ=255) gATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCa < 5:287524/137‑1 (MQ=255) gATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCa > 2:263924/1‑137 (MQ=255) gATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCa < 7:38362/137‑1 (MQ=255) aTCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCaaa < 8:39287/138‑1 (MQ=255) tCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATc > 6:306923/1‑134 (MQ=255) tCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATc < 5:306923/134‑1 (MQ=255) tCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGc > 6:78616/1‑139 (MQ=255) tCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGc < 2:194846/139‑1 (MQ=255) tCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGc > 1:194846/1‑139 (MQ=255) tGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCaa > 8:36642/1‑138 (MQ=255) aaTGGAC‑GTATTAAAAATTCAAATGTAATGATTAATCTCCCGAAATACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCa < 3:120456/139‑1 (MQ=255) aaTGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCa < 3:166454/139‑1 (MQ=255) aTGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCaa < 8:137742/139‑1 (MQ=255) | GATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAA > CP006881/1655856‑1656111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |