Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,579,785 Δ1 bp 100% coding (73/132 nt) U712_18380 → Hypothetical Protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,579,7850A.97.0% 281.2 / 1.8 66coding (73/132 nt)U712_18380Hypothetical Protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (40/24);  total (41/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

AAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTATT  >  CP006881/3579650‑3579919
                                                                                                                                       |                                                                                                                                      
aagcaagcATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaa                                                                                                                                       <  7:123952/137‑1 (MQ=255)
    acagaTACAATAGACATCTCTAGTAGAGGAAATCAACAAATCAACGCTGTTAACCAGAAATTAATATCAAAAAAACGTATGAAGTCGTAATCAATCCGAAACCGCACTTTTTATTTCGTGTGTCTGCATGCaaaaaaca                                                                                                                                 <  8:4759/135‑3 (MQ=255)
     agcaTACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAACCCGCACTTTTTATTTCTGGTCTCTGAATGCaaaaaaca                                                                                                                                 >  3:22229/1‑136 (MQ=255)
     agcaTACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaaaaaca                                                                                                                                 >  8:234006/1‑136 (MQ=255)
                 aCATCTCTAGTAGAGGAAATCAACAAATCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTATTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                                   >  2:166351/1‑139 (MQ=255)
                 aCATCTCTAGTAGAGGAAATCAACAAAGCACCGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                                   >  5:321641/1‑139 (MQ=255)
                 aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                                   >  1:66013/1‑139 (MQ=255)
                 aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                                   >  5:298050/1‑139 (MQ=255)
                   agaacaagtatAGGAAACAAAAAAAGCAACCCTGTTACCACTAAATTAAAATAAAAAAAACGCATGAGGTCGGAAGCAATCGTAAACGGAAC‑TTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTgg                                                                                                                <  8:347818/133‑1 (MQ=255)
                   aTCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCTGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCgctgt                                                                                                                 >  7:4759/1‑135 (MQ=255)
                   aTCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTg                                                                                                                 >  5:36049/1‑139 (MQ=255)
                   aTCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTg                                                                                                                 >  5:31624/1‑139 (MQ=255)
                     ctctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTgg                                                                                                                >  6:197574/1‑138 (MQ=255)
                      tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                               >  7:72105/1‑138 (MQ=255)
                      tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                               >  7:53334/1‑138 (MQ=255)
                      tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                               >  7:49065/1‑138 (MQ=255)
                      tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                               >  4:106799/1‑138 (MQ=255)
                      tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                               >  2:68558/1‑138 (MQ=255)
                      tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                               >  7:314066/1‑138 (MQ=255)
                          gtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAt                                                                                                           <  2:66013/138‑1 (MQ=255)
                             gagGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtctc                                                                                                        >  7:152583/1‑138 (MQ=255)
                               ggAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaa                                                                                                      >  2:126277/1‑138 (MQ=255)
                               ggAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaa                                                                                                      >  4:181561/1‑138 (MQ=255)
                                gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAACCCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTccac                                                                                                     >  1:266206/1‑138 (MQ=255)
                                gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                     >  7:63838/1‑138 (MQ=255)
                                gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                     >  8:110775/1‑138 (MQ=255)
                                gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAAACCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                     >  5:37604/1‑138 (MQ=255)
                                  aaTCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAAt                                                                                                  >  3:24554/1‑139 (MQ=255)
                                    tCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATcc                                                                                                <  4:24554/139‑1 (MQ=255)
                                    tCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATcc                                                                                                <  7:26202/139‑1 (MQ=255)
                                    tCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATcc                                                                                                <  5:39489/139‑1 (MQ=255)
                                             cAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTGTTTATTTCGGGAGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGCAAAATCTCAACAATCCGGGTg                                                                                           >  2:118254/1‑135 (MQ=255)
                                                cGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtct                                                                                    >  3:23697/1‑139 (MQ=255)
                                                     ttACCCAGAAATGAATATCAAAATAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCTGATcttctt                                                                                >  2:109719/1‑138 (MQ=255)
                                                     ttACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATcttctt                                                                                >  6:276193/1‑138 (MQ=255)
                                                     ttACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGAATGCAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGATCAGATctactt                                                                                >  6:298859/1‑139 (MQ=255)
                                                             aaaTGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAtt                                                                       >  5:38460/1‑139 (MQ=255)
                                                             aaaTGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCCGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAtt                                                                       >  6:137392/1‑139 (MQ=255)
                                                              aaTGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAttt                                                                      <  6:9927/139‑1 (MQ=255)
                                                                  aaTATCATAAAAACGGATGTAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAtttttt                                                                   <  4:22229/138‑1 (MQ=255)
                                                                  aaTATCAAATAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGGGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAtttttt                                                                   <  5:92115/138‑1 (MQ=255)
                                                                  aaTATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAttttttt                                                                  >  4:138173/1‑139 (MQ=255)
                                                                  aaTATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATATCACCAATCCGGGTGCACATCTTCTTTAATAGAttttttt                                                                  >  6:310026/1‑139 (MQ=255)
                                                                   atatCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTg                                                                 >  8:76804/1‑139 (MQ=255)
                                                                    tatCAAAAAAACGGATGAAGTCGTAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGc                                                                <  3:17940/139‑1 (MQ=255)
                                                                      tCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgt                                                              <  8:49065/139‑1 (MQ=255)
                                                                       cAAAAAAACGGATGAAGTCGGAATCAAGCCGAAACCGCACTTTTTATTTCGGTTGTCTGCATGC‑AAAAAACACACCCGGATCGGCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgtc                                                             >  4:125951/1‑139 (MQ=255)
                                                                           aaaaCGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgtctgtc                                                         <  8:72105/139‑1 (MQ=255)
                                                                            aaaCGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgtctgtct                                                        >  5:249132/1‑139 (MQ=255)
                                                                                ggATGATGTCGGAATCAATCCGAAACCGAACTTTTTATTTCGGGTGTCTGCATGAAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTcc                                                     <  5:298859/139‑1 (MQ=255)
                                                                                     aaGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTc                                               <  3:138173/139‑1 (MQ=255)
                                                                                          ggAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATcc                                                                                                <  3:339071/85‑1 (MQ=255)
                                                                                          ggAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATcc                                                                                                >  4:339071/1‑85 (MQ=255)
                                                                                          ggAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCaaaaa                                          <  1:126277/139‑1 (MQ=255)
                                                                                          ggAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCaaaaa                                          <  6:38460/139‑1 (MQ=255)
                                                                                            aatcaatcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAAt                                         >  1:125496/1‑138 (MQ=255)
                                                                                               caatcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaa                                     <  5:197574/139‑1 (MQ=255)
                                                                                                 atcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaac                                    >  4:347313/1‑138 (MQ=255)
                                                                                                 atcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaac                                    >  3:49118/1‑138 (MQ=255)
                                                                                                      aaaCCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACt                              >  7:296142/1‑139 (MQ=255)
                                                                                                      aaaCCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACt                              >  5:249990/1‑139 (MQ=255)
                                                                                                         ccGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCg                           <  7:110775/139‑1 (MQ=255)
                                                                                                           gCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCa                         <  6:31624/139‑1 (MQ=255)
                                                                                                           gCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCa                         <  6:298050/139‑1 (MQ=255)
                                                                                                            cACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCAt                        >  7:54655/1‑139 (MQ=255)
                                                                                                                  ttATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCaa                   <  3:181561/138‑1 (MQ=255)
                                                                                                                      ttCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAgg               <  2:143381/138‑1 (MQ=255)
                                                                                                                        cGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGct             <  6:189592/138‑1 (MQ=255)
                                                                                                                          ggTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCtt          <  5:123157/139‑1 (MQ=255)
                                                                                                                            tgtCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTcc        <  8:314066/139‑1 (MQ=255)
                                                                                                                                  catgc‑aAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTAtt  >  2:145800/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
AAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTATT  >  CP006881/3579650‑3579919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑