Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,720,161 A→C 6.5% F448L (TTT→TTG)  U712_19185 ← Sucrose‑6‑phosphate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,720,1610AC 6.5% 226.7 / 8.2 77F448L (TTT→TTG) U712_19185Sucrose‑6‑phosphate hydrolase
Reads supporting (aligned to +/- strand):  ref base A (32/40);  new base C (3/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

CAGATGGCTCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGTCTCGCTTCTGTTCTTTTTCCGTCAAAGCTCTTTCGTTCCAGC  >  CP006881/3720025‑3720293
                                                                                                                                        |                                                                                                                                    
caGATGGCTCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTaaa                                                                                                                                    <  4:69976/139‑1 (MQ=255)
   taggCTCAGATTTCTCATAAGAAAATAGAAGGATTTTCTACATACGTGTCCAAATTCAGACATTCTTTTCAGATTTCCCGGTCGCACTGGCTGTTAATTCATGATTTCCCGTGAAAGGAAAATATCTTGCACTAATGAc                                                                                                                                 <  8:142129/137‑1 (MQ=255)
   aTGGCTCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGTTCGCACTGGCTGTTACTACATGAATTCACGGGAAAGTAAAATATCTTGCAAGAAAGAc                                                                                                                                 >  5:47252/1‑139 (MQ=255)
   aTGGCTCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAgcc                                                                                                                                 >  2:204830/1‑137 (MQ=255)
   aTGGCTCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGAc                                                                                                                                 >  1:170360/1‑139 (MQ=255)
   aTGGCTCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGAc                                                                                                                                 >  3:33588/1‑139 (MQ=255)
   aTGGCTCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGAc                                                                                                                                 >  4:111429/1‑139 (MQ=255)
   aTGGCTCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGAc                                                                                                                                 >  4:216722/1‑139 (MQ=255)
   aTGGCTCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGAc                                                                                                                                 >  5:285810/1‑139 (MQ=255)
   aTGGCTCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGAc                                                                                                                                 >  6:12115/1‑139 (MQ=255)
   aTGGCTCAGAATACACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTACGACATTCATTTCAGATTTCCAGCTCGCACTGGCTGTTACTTCATGATTGCCCGGGAAAGGATAATATCTTGAACTAAAGAc                                                                                                                                 >  1:219678/1‑139 (MQ=255)
       cTCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACctct                                                                                                                             >  8:85830/1‑139 (MQ=255)
        tCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGAGATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACctct                                                                                                                             >  2:52762/1‑138 (MQ=255)
        tCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTCAAGACCtctt                                                                                                                            >  5:36097/1‑139 (MQ=255)
               tCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGt                                                                                                                      <  1:292883/138‑1 (MQ=255)
                cacaTAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGtt                                                                                                                     <  4:143036/138‑1 (MQ=255)
                cacaTAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTa                                                                                                                    >  2:310787/1‑139 (MQ=255)
                 acaTAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTa                                                                                                                    <  2:31600/138‑1 (MQ=255)
                 acaTAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTAt                                                                                                                   >  8:281133/1‑139 (MQ=255)
                           aTAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTGTTCTCCGTTATTGATAAAGAt                                                                                                         <  1:310787/139‑1 (MQ=255)
                               aaGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTc                                                                                                      >  1:336033/1‑138 (MQ=255)
                                           cATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGAc                                                                                            <  1:216532/136‑1 (MQ=255)
                                           cATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGAc                                                                                            <  2:338452/136‑1 (MQ=255)
                                            aTAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGt                                                                                        <  2:345489/139‑1 (MQ=255)
                                              aaGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTCAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTc                                                                                       >  3:49734/1‑138 (MQ=255)
                                               aGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCg                                                                                      <  2:198067/138‑1 (MQ=255)
                                                 tgtCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTCAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCgag                                                                                    >  3:321222/1‑138 (MQ=255)
                                                 tgtCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCgaga                                                                                   <  3:111429/139‑1 (MQ=255)
                                                   tCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCTCTGGCTGTTACTTCATGATTTCCCGGGATAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGCTGCCGCGTCGAGaaa                                                                                 >  3:296130/1‑139 (MQ=255)
                                                   tCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTCAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGaaa                                                                                 <  2:103205/139‑1 (MQ=255)
                                                   tCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACTCGTCGAGaaa                                                                                 >  7:240986/1‑139 (MQ=255)
                                                   tCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGaaa                                                                                 >  5:255799/1‑139 (MQ=255)
                                                   tCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGaaa                                                                                 <  2:336033/139‑1 (MQ=255)
                                                   tCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGaaa                                                                                 >  7:39766/1‑139 (MQ=255)
                                                   tCCAAATTCCGAAATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGaaa                                                                                 <  1:52762/139‑1 (MQ=255)
                                                      aaaTTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACAt                                                                              <  6:285810/139‑1 (MQ=255)
                                                            cgtccttcattTCAGATTTCCCGGTCCCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAAAATCTTCCACTAAAGACCTCTAATCCGTTATTGATAATGATTTCCACAGATGACGCGTCGAGAAACATGTGTa                                                                         <  2:219678/133‑1 (MQ=255)
                                                            cGACATTCATTTCAGATTTGCCGGTCGCACTGGCTTTTACTTCATGATTTCTCTGTAAAGTAAATTATCTTGCACTAAAGATATATTATCCTTTTTTTATAAAGATTTCCACAGATGACGCGTCTAGAAACATGTGTa                                                                         <  6:313540/138‑1 (MQ=255)
                                                            cGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTa                                                                         <  7:281133/138‑1 (MQ=255)
                                                            cGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTa                                                                         <  2:170360/138‑1 (MQ=255)
                                                            cGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTa                                                                         <  1:4556/138‑1 (MQ=255)
                                                            cGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTa                                                                         <  8:39766/138‑1 (MQ=255)
                                                            cGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTAc                                                                        <  1:310288/139‑1 (MQ=255)
                                                             gACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGTAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACg                                                                       <  5:248573/139‑1 (MQ=255)
                                                             gACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTCAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACg                                                                       <  4:321222/139‑1 (MQ=255)
                                                              aCATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACgg                                                                      <  3:308599/139‑1 (MQ=255)
                                                                    attTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCa                                                                <  2:313697/139‑1 (MQ=255)
                                                                    attTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCa                                                                <  3:216722/139‑1 (MQ=255)
                                                                    attTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCa                                                                <  4:103922/139‑1 (MQ=255)
                                                                    attTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCa                                                                <  7:85830/139‑1 (MQ=255)
                                                                    attTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCa                                                                <  5:12115/139‑1 (MQ=255)
                                                                    attTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCa                                                                <  5:155914/139‑1 (MQ=255)
                                                                                ccGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGc                                                    >  6:183213/1‑139 (MQ=255)
                                                                                            gCTGGTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATATAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGtct                                          <  6:183279/137‑1 (MQ=255)
                                                                                             cTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGTCTCGc                                       <  8:240986/139‑1 (MQ=255)
                                                                                              tGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCa                                                                <  6:260728/113‑1 (MQ=255)
                                                                                              tGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCa                                                                >  5:260728/1‑113 (MQ=255)
                                                                                                  aCTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGTCTCGCTTCTg                                  >  6:132457/1‑139 (MQ=255)
                                                                                                      cATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCca                                                                                                    <  7:107477/69‑1 (MQ=255)
                                                                                                      cATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCca                                                                                                    >  8:107477/1‑69 (MQ=255)
                                                                                                      cATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTa                                                                         >  6:204307/1‑96 (MQ=255)
                                                                                                      cATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTa                                                                         >  5:290980/1‑96 (MQ=255)
                                                                                                      cATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTa                                                                         <  5:204307/96‑1 (MQ=255)
                                                                                                      cATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTa                                                                         <  6:290980/96‑1 (MQ=255)
                                                                                                      cATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGTCTCGCTTCTGTTCt                              >  4:45384/1‑139 (MQ=255)
                                                                                                      cATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCCTTATTGATACAGATTTCCACACATGACGCGTCGAGAAACATGTGTACGGTATGCCAATCCTTGCTGCGGCAATGTCTCGCTTCGGtgat                              >  7:303046/1‑136 (MQ=255)
                                                                                                      cATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCGTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGTCTCGCTTCTGTTCt                              >  7:236388/1‑139 (MQ=255)
                                                                                                              cccGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTa                                                                         >  7:44725/1‑88 (MQ=255)
                                                                                                              cccGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTa                                                                         <  8:44725/88‑1 (MQ=255)
                                                                                                              cccGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGTCTCGCTTCTGTTCTTTTTCCGt                      >  8:127682/1‑139 (MQ=255)
                                                                                                               ccGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGAGGACGCGTCGAGAATCATTTGTACGGTATGCAAATCCTTGATGCGGCAATGTCTCGCTTCTGTTCTTTTTCCGt                      <  6:47252/138‑1 (MQ=255)
                                                                                                                        gaaaATATCTTGCACTACAGACCTCTTCTACGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGTCTCGCTTCTGTTCTTTTTCCGTCAAAGCTCtt            <  4:33588/139‑1 (MQ=255)
                                                                                                                             tatCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGTCTCGCTTCTGTTCTTTTTCCGTCAAAGCTCTTTCGtt       <  5:183213/139‑1 (MQ=255)
                                                                                                                                  tGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGc                                                 >  8:196617/1‑92 (MQ=255)
                                                                                                                                  tGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGc                                                 <  7:196617/92‑1 (MQ=255)
                                                                                                                                  tGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGtct                                          >  7:71212/1‑99 (MQ=255)
                                                                                                                                  tGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGtct                                          <  8:71212/99‑1 (MQ=255)
                                                                                                                                  tGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGTCTCGCTTCTGTTCTTTTTCCGTCAAAGGTCTTTCGTTCCAGc  >  7:41676/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                    
CAGATGGCTCAGATTTCACATAAGAAAATAGAAGGATTTTCTACATAAGTGTCCAAATTCCGACATTCATTTCAGATTTCCCGGTCGCACTGGCTGTTACTTCATGATTTCCCGGGAAAGGAAAATATCTTGCACTAAAGACCTCTTCTCCGTTATTGATAAAGATTTCCACAGATGACGCGTCGAGAAACATGTGTACGGTATGCAAATCCTTGATGCGGCAATGTCTCGCTTCTGTTCTTTTTCCGTCAAAGCTCTTTCGTTCCAGC  >  CP006881/3720025‑3720293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑