Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,722,386 A→G 5.5% S167S (AGT→AGC)  U712_19190 ← PTS system sucrose‑specific EIIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,722,3860AG 5.5% 265.9 / 4.4 91S167S (AGT→AGC) U712_19190PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base A (40/45);  new base G (2/3);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

ATCAAATCCGAACAGATGCATATAATCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCATTAATAGGCCGCTGGC  >  CP006881/3722254‑3722523
                                                                                                                                    |                                                                                                                                         
atCAAATCCGAACAGATGCATATAATCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGaca                                                                                                                                     <  5:306598/139‑1 (MQ=255)
    aaTCCGAACAGATGCATATAATCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGa                                                                                                                                 >  2:23804/1‑139 (MQ=255)
          aaCAGATGCATATAATCAGGGGTTGCTTCCGCCAAACCCCAGGGATTCAAAAGCGTCGGATGAAGCATGATACATCCGATGACCGGGCCCAAGAAAGGGTTGGTTCCAAAAGCTTTTGAAGCAGTGACACCGATTaaa                                                                                                                            >  7:346902/1‑138 (MQ=255)
               aTGCATATAATCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCgg                                                                                                                       >  7:38257/1‑138 (MQ=255)
                       aaTCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGAGTAAAATCGGCAAGaaaa                                                                                                               >  1:1034/1‑138 (MQ=255)
                            ggggTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATg                                                                                                         >  4:294479/1‑139 (MQ=255)
                             gggTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGCCCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGc                                                                                                        >  2:83997/1‑139 (MQ=255)
                             gggTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGc                                                                                                        >  5:148927/1‑139 (MQ=255)
                             gggTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGc                                                                                                        >  1:228706/1‑139 (MQ=255)
                             gggTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGc                                                                                                        <  1:227387/139‑1 (MQ=255)
                             gggTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGc                                                                                                        >  1:76207/1‑139 (MQ=255)
                             gggTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGc                                                                                                        >  6:82105/1‑139 (MQ=255)
                             gggTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGc                                                                                                        >  6:41394/1‑139 (MQ=255)
                             gggTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGc                                                                                                        >  2:227387/1‑139 (MQ=255)
                             gggTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATGAAAATCGGCAAGAAAATAAATGc                                                                                                        >  2:78769/1‑139 (MQ=255)
                             gggTTGCTTCCGCCAAACCCCATGGATACAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGc                                                                                                        >  4:159222/1‑139 (MQ=255)
                               gTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCg                                                                                                      <  5:18183/139‑1 (MQ=255)
                               gTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCg                                                                                                      <  1:23804/139‑1 (MQ=255)
                               gTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCg                                                                                                      <  5:163402/139‑1 (MQ=255)
                                  gCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGCTACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGACGCAc                                                                                                   >  1:294128/1‑139 (MQ=255)
                                   cTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCATCCTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACt                                                                                                  >  5:194982/1‑139 (MQ=255)
                                        gCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTgaga                                                                                             <  3:173796/139‑1 (MQ=255)
                                           aaaCCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACa                                                                                          <  3:177270/139‑1 (MQ=255)
                                                 cATTGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGa                                                                                    <  2:230152/139‑1 (MQ=255)
                                                 cATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGa                                                                                    <  6:302202/139‑1 (MQ=255)
                                                 cATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTACAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGa                                                                                    <  2:76207/139‑1 (MQ=255)
                                                   tGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCa                                                                                                                                           >  8:68987/1‑82 (MQ=255)
                                                   tGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCa                                                                                                                                           <  7:68987/82‑1 (MQ=255)
                                                   tGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAaaataaa                                                                                                           >  6:11638/1‑114 (MQ=255)
                                                   tGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAaaataaa                                                                                                           <  5:11638/114‑1 (MQ=255)
                                                    ggATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGagc                                                                                  <  2:339037/138‑1 (MQ=255)
                                                         cAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCCCTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTgc                                                                            >  8:80499/1‑139 (MQ=255)
                                                             aGCGGCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAATATGTCGAGCAGCTgcagca                                                                        <  8:346902/139‑1 (MQ=255)
                                                               cGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCagcagc                                                                      >  1:21777/1‑139 (MQ=255)
                                                                 tCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGc                                                                                                                      >  2:72393/1‑89 (MQ=255)
                                                                 tCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGc                                                                                                                      <  1:72393/89‑1 (MQ=255)
                                                                   ggATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCa                                                                   <  6:148927/138‑1 (MQ=255)
                                                                   ggAAGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAg                                                                  <  4:228730/139‑1 (MQ=255)
                                                                      tGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAAt                                                               <  2:120336/139‑1 (MQ=255)
                                                                            aTGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGc                                                         <  8:38257/139‑1 (MQ=255)
                                                                                  ccTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATc                                                    <  3:154343/138‑1 (MQ=255)
                                                                                   cTCCGCTGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTGTTGACGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGACGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATcc                                                   >  2:258283/1‑138 (MQ=255)
                                                                                     ccGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAAt                                                >  7:4465/1‑139 (MQ=255)
                                                                                        aTGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGaa                                             <  4:140674/139‑1 (MQ=255)
                                                                                        aTGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGa                                              >  2:242875/1‑138 (MQ=255)
                                                                                        aTGACAGCGCCCCAGTAAGGGTTGCCTCCAAACTCTTTTGACGCACGGACACCGATTAACATCGGCAAGAAAATAAAAGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTAGCTCATCCAATGaa                                             <  6:23463/139‑1 (MQ=255)
                                                                                             cGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCa                                        <  3:294479/139‑1 (MQ=255)
                                                                                             cGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCa                                        >  7:72115/1‑139 (MQ=255)
                                                                                             cGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCa                                        <  2:294128/139‑1 (MQ=255)
                                                                                               cTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCAtt                                      >  3:150166/1‑139 (MQ=255)
                                                                                               cTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAACTGCCGCACTTGAGCACATGTCGAGCAGCTGCAGCAGCGCAGCATCCTTGCTCATCCAATGAAACGCAtt                                      >  5:71663/1‑139 (MQ=255)
                                                                                               cTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCa                                        >  6:293440/1‑137 (MQ=255)
                                                                                               cTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCa                                        <  5:293440/137‑1 (MQ=255)
                                                                                               cTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCAtt                                      >  1:242854/1‑139 (MQ=255)
                                                                                               cTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCAAATGAAACGCAtt                                      >  8:314891/1‑139 (MQ=255)
                                                                                                tCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCAtt                                      >  4:247917/1‑138 (MQ=255)
                                                                                                 cccAAGTAAGGGGTGCTTCCTAACTCTTTTGAAGCACTGACACCAATTAAAATAGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGa                                    <  8:295219/139‑1 (MQ=255)
                                                                                                  ccAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGAt                                   <  2:21777/139‑1 (MQ=255)
                                                                                                      gTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCTATTAAAATCGGCAAGAAAATAAAAGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATa                               <  1:53714/139‑1 (MQ=255)
                                                                                                      gTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGTTTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATa                               <  3:6457/139‑1 (MQ=255)
                                                                                                      gTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATa                               <  2:228706/139‑1 (MQ=255)
                                                                                                      gTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATa                               <  5:82105/139‑1 (MQ=255)
                                                                                                      gTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATa                               <  8:4465/139‑1 (MQ=255)
                                                                                                          gggTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTaaa                                                                                                                            <  5:178109/42‑1 (MQ=255)
                                                                                                          gggTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTaaa                                                                                                                            >  6:178109/1‑42 (MQ=255)
                                                                                                          gggTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGagc                                                                                  <  2:40020/84‑1 (MQ=255)
                                                                                                          gggTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGagc                                                                                  >  1:40020/1‑84 (MQ=255)
                                                                                                          gggTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCa                                                                   >  2:348344/1‑99 (MQ=255)
                                                                                                          gggTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCa                                                                   <  1:348344/99‑1 (MQ=255)
                                                                                                          gggTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATAccc                            >  7:64166/1‑138 (MQ=255)
                                                                                                          gggTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATAccc                            >  2:285716/1‑138 (MQ=255)
                                                                                                            gTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCAAATGAAACGCATTGATCATAcaaaa                          >  3:38736/1‑134 (MQ=255)
                                                                                                             ttGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCaata                        <  3:159222/139‑1 (MQ=255)
                                                                                                              tgctgcCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCagcaa                       >  2:209316/1‑135 (MQ=255)
                                                                                                              tgattCCAAACTCTTTTGAAGCACTGACACAGATTAAAATCGTAAAGAAAATAAATGACTAACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCaata                        <  6:23507/135‑1 (MQ=255)
                                                                                                              tGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTgaga                                                                                             <  2:331965/69‑1 (MQ=255)
                                                                                                              tGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTgaga                                                                                             >  1:331965/1‑69 (MQ=255)
                                                                                                               gCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATa                               >  4:78250/1‑130 (MQ=255)
                                                                                                               gCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATa                               <  3:78250/130‑1 (MQ=255)
                                                                                                               gCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCaataat                      >  1:116744/1‑139 (MQ=255)
                                                                                                                cTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCg                                                                                                      >  6:11772/1‑58 (MQ=255)
                                                                                                                cTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCg                                                                                                      <  5:11772/58‑1 (MQ=255)
                                                                                                                   ccAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATcc                    <  4:150166/137‑1 (MQ=255)
                                                                                                                     aaaCTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCa                  <  6:117486/137‑1 (MQ=255)
                                                                                                                     aaaCTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCa                  >  5:117486/1‑137 (MQ=255)
                                                                                                                               gAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCATTAATAGGCCg       <  1:242875/138‑1 (MQ=255)
                                                                                                                               gAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCATTAATAGGCCg       <  3:247917/138‑1 (MQ=255)
                                                                                                                                   cACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATa                               <  6:309144/110‑1 (MQ=255)
                                                                                                                                   cACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATa                               >  5:309144/1‑110 (MQ=255)
                                                                                                                                   cACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCATTAATAGGCCGCTGGc  <  5:41394/139‑1 (MQ=255)
                                                                                                                                   cACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCATTAATAGGCCGCTGGc  <  1:258283/139‑1 (MQ=255)
                                                                                                                                   cACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCATTAATAGGCCGCTGGc  <  8:64166/139‑1 (MQ=255)
                                                                                                                                   cACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCATTAATAGGCCGCTGGc  <  3:296811/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                         
ATCAAATCCGAACAGATGCATATAATCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCATTAATAGGCCGCTGGC  >  CP006881/3722254‑3722523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑