Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,723,888	A→G	100%	T234T (ACA→ACG)	U712_19195 →	putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,888	0	A	G	100.0%	220.0 / ‑6.6	62	T234T (ACA→ACG)	U712_19195	putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (34/27);  minor base T (0/1);  total (34/28)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTG  >  CP006881/3723754‑3724022                                                                                                                                       |                                                                                                                                       ttCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTc                                                                                                                                     <  6:159803/138‑1 (MQ=255) ttCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCg                                                                                                                                    >  5:218968/1‑139 (MQ=255)        tCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCt                                                                                                                             >  8:271797/1‑139 (MQ=255)               ctttATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACcgc                                                                                                                       <  1:178977/138‑1 (MQ=255)               ctttATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACcgc                                                                                                                       >  2:178977/1‑138 (MQ=255)                  taTTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCggg                                                                                                                    <  3:347564/137‑1 (MQ=255)                                  cacaGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCt                                                                                                   <  7:271797/139‑1 (MQ=255)                                      gCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                                                                                               <  7:100582/139‑1 (MQ=255)                                      gCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                                                                                               <  6:103961/139‑1 (MQ=255)                                             cAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGtactac                                                                                        <  4:278203/139‑1 (MQ=255)                                                 atGTGCACATTCACCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTCACCGCGGGAATAGTTATGATGGGCTGCATGTCCTacacac                                                                                    >  6:47876/1‑137 (MQ=255)                                                  tgtgCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGTAACTTAATCCCCGTTACTCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACt                                                                                   <  5:204839/139‑1 (MQ=255)                                                   gtgCACATTCACCATCTCGCTTTTGATCGAGCTCCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGTGAATAGTTATGATGGGCTGGATGTACTACACACt                                                                                   <  5:47876/138‑1 (MQ=255)                                                         aTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATcct                                                                            >  8:304970/1‑138 (MQ=255)                                                                  ctcGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGatcaata                                                                    >  3:331758/1‑138 (MQ=255)                                                                     gcTTTTGATCGAGCACCCTGATACAGGGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATaaaaa                                                                <  6:180397/138‑1 (MQ=255)                                                                         ttGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTACTCCCCGTTACGCTCGGCAATCTGACCGCGGTAATAGTTAAGATGGGCTGGATGTACTACACACTGAATCCTGATCaaaaaaaaaa                                                              <  5:57040/137‑1 (MQ=255)                                                                                 gCACCCTGATACTGTGACAGTTATGGGAGCAGTCAGAAACTTAATCCCCGTTCCGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACAACACACTGAATCCTTATCAATAAAAACACTTCCAgaga                                                    <  2:136112/139‑1 (MQ=255)                                                                                 gCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTgg                                                                                                 >  6:234432/1‑94 (MQ=255)                                                                                 gCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTgg                                                                                                 <  5:234432/94‑1 (MQ=255)                                                                                       taaTACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTc                                              >  4:337023/3‑139 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGCCCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                      >  7:240296/1‑139 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                                                                  >  3:143852/1‑63 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                                                                  >  7:248481/1‑63 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                                                                  <  4:143852/63‑1 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                                                                  <  8:248481/63‑1 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTa                                                                                            <  5:35848/85‑1 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTa                                                                                            >  6:35848/1‑85 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                      >  5:346570/1‑139 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                      >  7:62922/1‑139 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                      >  7:129322/1‑139 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                      >  3:8986/1‑139 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                      >  8:36623/1‑139 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                      >  8:308485/1‑139 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                      >  2:84238/1‑139 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCAAGAGATCACTCCCTGCAAg                                      >  8:55850/1‑139 (MQ=255)                                                                                               tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAACCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                      >  1:197011/1‑139 (MQ=255)                                                                                                   actgttGGGAGCAGTCAGAAAATTAATCCCGGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTTATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                  <  4:331758/134‑1 (MQ=255)                                                                                                   actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCATTAAAAAAACTTCCAGAGATCCCTCCCTGCAAGTCa                                   >  8:132104/5‑138 (MQ=255)                                                                                                   actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                  <  5:120226/135‑1 (MQ=255)                                                                                                   actgaTGGGAGCAGTCAGAAACTAAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCa                                   >  2:325994/5‑138 (MQ=255)                                                                                                       aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCg                              >  7:160320/1‑139 (MQ=255)                                                                                                           gAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatgatg                                                                                                       <  3:245226/62‑1 (MQ=255)                                                                                                           gAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatgatg                                                                                                       >  4:245226/1‑62 (MQ=255)                                                                                                               aGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCTCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAAGAAAAAAACTTCCAGAGATCACTCCCTGCAAGACAAAGCGGTAATTcc                      >  2:188196/1‑139 (MQ=255)                                                                                                                 tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATTGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgc                    >  6:84440/1‑138 (MQ=255)                                                                                                                 tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTa                                                                                         <  6:18246/70‑1 (MQ=255)                                                                                                                 tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc                      >  6:27160/1‑137 (MQ=255)                                                                                                                 tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc                      >  5:59101/1‑137 (MQ=255)                                                                                                                 tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc                      >  6:5470/1‑137 (MQ=255)                                                                                                                 tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc                      >  8:241606/1‑137 (MQ=255)                                                                                                                  cAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAAAAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                   <  7:304970/139‑1 (MQ=255)                                                                                                                    gAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGGGGTAATAGTTATTATGGGCTGGATCTACTACAAACTGAATCCTTATCAATAAAAACACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgagag                 <  6:218968/139‑1 (MQ=255)                                                                                                                    gAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAACCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgagag                 <  2:197011/139‑1 (MQ=255)                                                                                                                            atcccggTTACGCTCGGAAATCTGACCGCGGTATTATTTATTATGGGCTGGATGTAATAAACACTTAATCCTGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGaa         <  8:129322/133‑1 (MQ=255)                                                                                                                               cccGTTACGCTCGGCAATCTGACCGCGGTAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc      <  8:62922/139‑1 (MQ=255)                                                                                                                               cccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc      >  4:110599/1‑139 (MQ=255)                                                                                                                                 cGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGc    <  3:337023/139‑1 (MQ=255)                                                                                                                                 cGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGc    >  8:160012/1‑139 (MQ=255)                                                                                                                                 cGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGc    <  7:308485/139‑1 (MQ=255)                                                                                                                                  gTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCt   >  5:132731/1‑139 (MQ=255)                                                                                                                                   ttACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGTGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTg  <  7:55850/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTG  >  CP006881/3723754‑3724022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑