Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,724,446 G→A 100% Y127Y (TAC→TAT)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,4460GA100.0% 334.5 / NA 98Y127Y (TAC→TAT) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/50);  total (48/50)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGC  >  CP006881/3724311‑3724580
                                                                                                                                       |                                                                                                                                      
cTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATa                                                                                                                                      <  8:236578/138‑1 (MQ=255)
 ttCAGCGCTGAATACAGGCTCTCCGCAGCCATCTTCGCCGTATGGATGGGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATAtt                                                                                                                                    <  2:24903/139‑1 (MQ=255)
   cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGCGACACGCCCAATGTCTCGTTTACATTTCCGATCGCCCACAGGCCGATCTCATATTCt                                                                                                                                  >  7:241739/1‑139 (MQ=255)
   cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCt                                                                                                                                  >  1:146656/1‑139 (MQ=255)
    aGCGCTGAATAGATGCTCTCCGCATCCATCTTCGCTGTATGGATGTGAAGTGCAATATAACCCGCTTCATCTTCAGGCAGAGAGACGCCCAATTTCTCTTTTACATGTCCGATCGCCCACAGGCAGATCTCATATTCt                                                                                                                                  <  2:232189/138‑1 (MQ=255)
    aGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCt                                                                                                                                  <  8:88759/138‑1 (MQ=255)
           aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTtata                                                                                                                          >  6:305384/1‑139 (MQ=255)
           aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTta                                                                                                                            >  4:123159/1‑137 (MQ=255)
           aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTta                                                                                                                            <  3:123159/137‑1 (MQ=255)
           aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGAGCTCATATTCTTTTTta                                                                                                                            >  3:199330/1‑137 (MQ=255)
           aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTGTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTta                                                                                                                            >  4:271542/1‑137 (MQ=255)
           aaTACATGCTCTCCGCATCCAGCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCCGGCAGAGACACGCCCAATGTCTCTTTTACATGTGCGATCGCCCACAGGCCGATCTCATATTCTTTTTta                                                                                                                            <  1:107614/137‑1 (MQ=255)
              aCATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  2:132678/139‑1 (MQ=255)
               cATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGTCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  7:227003/138‑1 (MQ=255)
               cATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  6:173632/138‑1 (MQ=255)
               cATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  8:326856/138‑1 (MQ=255)
               cATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  5:130841/138‑1 (MQ=255)
               cATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  1:74392/138‑1 (MQ=255)
               cATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAgc                                                                                                                      <  1:176940/139‑1 (MQ=255)
                  gCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAgcgcc                                                                                                                   >  1:123470/1‑138 (MQ=255)
                     ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCcttt                                                                                                                >  1:221187/1‑139 (MQ=255)
                     ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                                                                                 >  8:115685/1‑138 (MQ=255)
                     ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                                                                                 >  8:11508/1‑138 (MQ=255)
                       ccGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATAtt                                                                                                                                    <  7:340678/117‑1 (MQ=255)
                       ccGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATAtt                                                                                                                                    >  8:340678/1‑117 (MQ=255)
                        cGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTAtc                                                                                                             >  8:307805/1‑139 (MQ=255)
                        cGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTAtc                                                                                                             >  6:123774/1‑139 (MQ=255)
                        cGCATCCATCTTCCCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTTTAGAGCGCCTTTAtc                                                                                                             >  8:195093/1‑139 (MQ=255)
                             cagtcTTCGCCGTATGGAGGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATg                                                                                                        <  1:259161/136‑1 (MQ=255)
                              catcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgc                                                                                                       >  1:179987/1‑139 (MQ=255)
                              catcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATg                                                                                                        <  7:115685/138‑1 (MQ=255)
                              catcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATg                                                                                                        <  2:146656/138‑1 (MQ=255)
                              catcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATg                                                                                                        <  3:46508/138‑1 (MQ=255)
                               atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                                                                      <  5:176517/139‑1 (MQ=255)
                               atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                                                                      <  1:105153/139‑1 (MQ=255)
                               atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                                                                      <  1:14971/139‑1 (MQ=255)
                                tcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       >  6:252859/1‑121 (MQ=255)
                                tcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  5:252859/121‑1 (MQ=255)
                                tcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgcag                                                                                                     >  8:282079/1‑139 (MQ=255)
                                    cGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgcag                                                                                                     <  5:73321/135‑1 (MQ=255)
                                         tATGGATGTGAGGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTAt                                                                                             >  1:314146/1‑138 (MQ=255)
                                           tGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATg                                                                                                        >  2:296409/1‑125 (MQ=255)
                                           tGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATg                                                                                                        <  1:296409/125‑1 (MQ=255)
                                           tGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCt                                                                                          >  1:110916/1‑139 (MQ=255)
                                           tGGATGTGAAGGGCAATAAAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGAGACTATATCTCATGCAGAAATTTAtctg                                                                                          >  2:259161/1‑136 (MQ=255)
                                             gATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGc                                                                                        <  5:218426/139‑1 (MQ=255)
                                                 tgAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTAAAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGcac                                                                                      >  1:235297/1‑137 (MQ=255)
                                                   aaGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  7:280040/102‑1 (MQ=255)
                                                   aaGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       >  8:280040/1‑102 (MQ=255)
                                                   aaGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAag                                                                                   >  1:246701/1‑138 (MQ=255)
                                                      ggCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAagcagc                                                                               <  5:14483/139‑1 (MQ=255)
                                                       gCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                                                                              <  7:282079/139‑1 (MQ=255)
                                                        cAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                                                                              >  5:94512/1‑138 (MQ=255)
                                                         aaTATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       >  5:231954/1‑96 (MQ=255)
                                                         aaTATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  6:231954/96‑1 (MQ=255)
                                                          atatAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATCTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCACCCCAt                                                                           >  1:60495/1‑139 (MQ=255)
                                                           tataACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCAtt                                                                          <  1:27956/139‑1 (MQ=255)
                                                             taACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTAAAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCAtttt                                                                        <  2:235297/139‑1 (MQ=255)
                                                                cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       >  7:286693/1‑89 (MQ=255)
                                                                cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  8:286693/89‑1 (MQ=255)
                                                                     ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  8:10634/84‑1 (MQ=255)
                                                                     ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       >  7:10634/1‑84 (MQ=255)
                                                                     ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAgcgcct                                                                                                                  <  8:298150/89‑3 (MQ=255)
                                                                     ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAgcgcct                                                                                                                  >  7:298150/1‑87 (MQ=255)
                                                                     ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                >  6:92870/1‑139 (MQ=255)
                                                                     ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                >  8:12885/1‑139 (MQ=255)
                                                                     ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                >  2:321225/1‑139 (MQ=255)
                                                                     ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCACCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                >  8:171795/1‑139 (MQ=255)
                                                                     ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTAAATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                >  7:94533/1‑139 (MQ=255)
                                                                              aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCt                                                                                                                                  <  4:102155/64‑1 (MQ=255)
                                                                              aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCt                                                                                                                                  >  3:102155/1‑64 (MQ=255)
                                                                              aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCa                                                       <  1:321225/139‑1 (MQ=255)
                                                                                 cagagACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTTTAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCAGAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAg                                                    <  7:195093/138‑1 (MQ=255)
                                                                                    agaCACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCAGTTTGAATCCTTTCGATCGCAAAGGa                                                  >  5:255465/1‑138 (MQ=255)
                                                                                       cacGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACaaa                                              <  5:92870/139‑1 (MQ=255)
                                                                                       cacGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCACCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACaaa                                              <  7:171795/139‑1 (MQ=255)
                                                                                           cccAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCCTGCAGCAATTTATTCTGCCCAAGCAGCCCATTTTGATTCCTTTCGATCGCAAAGGACAAGTGGt                                          >  7:102371/1‑139 (MQ=255)
                                                                                               aTGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGa                                      <  5:305384/139‑1 (MQ=255)
                                                                                                tGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       >  1:130386/1‑57 (MQ=255)
                                                                                                tGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  2:130386/57‑1 (MQ=255)
                                                                                                      ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCATCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                 <  6:27843/137‑1 (MQ=255)
                                                                                                      ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                 <  7:307805/137‑1 (MQ=255)
                                                                                                      ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                 <  4:199330/137‑1 (MQ=255)
                                                                                                      ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                 <  2:179987/137‑1 (MQ=255)
                                                                                                      ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                 <  7:131884/137‑1 (MQ=255)
                                                                                                          aCATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       <  6:37389/47‑1 (MQ=255)
                                                                                                          aCATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                                                                       >  5:37389/1‑47 (MQ=255)
                                                                                                                ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                                                                              <  3:137551/82‑1 (MQ=255)
                                                                                                                ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                                                                              >  4:137551/1‑82 (MQ=255)
                                                                                                                ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACATATGGTCGGAAAGCGCGATATGAAtgt                     >  1:134787/1‑139 (MQ=255)
                                                                                                                ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAtgt                     >  5:131032/1‑139 (MQ=255)
                                                                                                                ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAtgt                     >  8:150570/1‑139 (MQ=255)
                                                                                                                  gATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAgcgcct                                                                                                                  <  4:328833/44‑3 (MQ=255)
                                                                                                                  gATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAgcgcct                                                                                                                  >  3:328833/1‑42 (MQ=255)
                                                                                                                         cacaGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCt              >  8:255678/1‑137 (MQ=255)
                                                                                                                                  aTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAg                                   >  4:225409/1‑107 (MQ=255)
                                                                                                                                  aTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAg                                   <  3:225409/107‑1 (MQ=255)
                                                                                                                                   tctcATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgccgc  >  4:348836/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGC  >  CP006881/3724311‑3724580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑