Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,724,623 A→T 100% I68I (ATT→ATA)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,6230AT100.0% 126.5 / ‑6.6 40I68I (ATT→ATA) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (20/19);  minor base G (1/0);  total (21/19)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCAT  >  CP006881/3724494‑3724757
                                                                                                                                 |                                                                                                                                      
cagaaGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCGCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctc                                                                                                                                <  6:262135/135‑1 (MQ=255)
cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGCCCTCTCCGTCCGCATAGCTGATGAGATCCGCAGCTATTTCTATATGctcc                                                                                                                               >  5:198834/1‑139 (MQ=255)
cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctcc                                                                                                                               >  1:37984/1‑139 (MQ=255)
cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGc                                                                                                                                  <  4:309475/136‑1 (MQ=255)
      cagcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGc                                                                                                                         <  8:111442/139‑1 (MQ=255)
       agcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCa                                                                                                                        <  7:255678/139‑1 (MQ=255)
        gcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGAGGATATCCTCAGCTATTTCGAGATGCTCCTCCGGCAg                                                                                                                       >  2:253592/1‑139 (MQ=255)
          ccATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAgtg                                                                                                                     >  5:123059/1‑139 (MQ=255)
               ttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTg                                                                                                                 <  6:123059/138‑1 (MQ=255)
                   aTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGa                                                                                                            <  6:122552/139‑1 (MQ=255)
                   aTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGa                                                                                                            >  6:153500/1‑139 (MQ=255)
                   aTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGa                                                                                                            <  6:128647/139‑1 (MQ=255)
                         tCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTACATCCGCATAGCTGATGATATCCTCAACTATTACTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGtt                                                                                                      >  4:110318/1‑139 (MQ=255)
                            aTCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGtttga                                                                                                   <  8:160493/139‑3 (MQ=255)
                            aTCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGGGTTTGCAGGATTTGtttg                                                                                                    >  3:263827/1‑137 (MQ=255)
                               gCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAAc                                                                                                 <  4:285474/138‑1 (MQ=255)
                                  aaGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACtttt                                                                                             <  7:150570/139‑1 (MQ=255)
                                               cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtcgtcgcg                                                                                >  3:310472/3‑137 (MQ=255)
                                               cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtcgtcgcg                                                                                >  7:22806/3‑137 (MQ=255)
                                                ggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtcgtcgcg                                                                                <  6:198834/137‑3 (MQ=255)
                                                 gAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCa                                                                              <  2:123470/139‑1 (MQ=255)
                                                        cgATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGTTTTGTTTGAACTTTTCATTATCGTCGCGCCCGACa                                                                         >  3:176191/1‑137 (MQ=255)
                                                                aTGTGATCGCTCAGCGGCGCCGCGCGCTCTCCTTCCGCATAGCTGATGATTTCCTCAGCTATTTCTATATGCTCCTCCGGCCGTGTTTGCAGGATTTGTTTGAAGTTTTCATTCTCGTCGCGCACGACAAAGATCtttt                                                               >  7:231949/1‑139 (MQ=255)
                                                                aTGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCtttt                                                               <  6:255465/139‑1 (MQ=255)
                                                                aTGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCtttt                                                               <  4:310472/139‑1 (MQ=255)
                                                                     aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                          <  2:134787/139‑1 (MQ=255)
                                                                     aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                          <  8:241739/139‑1 (MQ=255)
                                                                     aTCGCTCAGCGGCGCCGCGAGCTCTCCGTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                          >  7:294900/1‑139 (MQ=255)
                                                                         cTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATACTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGGTTTGTTTGAACTTTTCATTCTCGTCGCGCCCGACAAAGATGTTTTCCACTTTAt                                                      >  5:217506/1‑139 (MQ=255)
                                                                            aGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCa                                                   <  2:37984/139‑1 (MQ=255)
                                                                             gcggctCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCAt                                                  <  6:265616/139‑1 (MQ=255)
                                                                                                       tAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCttttttt                        >  6:232057/1‑139 (MQ=255)
                                                                                                         gCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTg                       >  3:6852/1‑138 (MQ=255)
                                                                                                             atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                          <  6:272769/99‑1 (MQ=255)
                                                                                                             atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                          >  5:272769/1‑99 (MQ=255)
                                                                                                                    ccTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCTTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATTAGATCATTTTTCTTTTTTTGAAAAGCGATTcc           >  7:60846/1‑139 (MQ=255)
                                                                                                                    ccTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTcc           >  2:86213/1‑139 (MQ=255)
                                                                                                                    ccTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTcc           >  5:122128/1‑139 (MQ=255)
                                                                                                                        aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTATGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATAATTTTTCTTTTTTTGAAAAGAGATTcacggt       >  1:137538/1‑134 (MQ=255)
                                                                                                                             tttCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTTTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCAATTTATTCATAGGGATGAGATAATTTTTCTTTTTTTGAAAAGCGATTCCAGGTCCCAt  >  7:298792/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                      
CACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCAT  >  CP006881/3724494‑3724757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑