Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,724,902	A→C	100%	A89A (GCT→GCG)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,724,902	0	A	C	100.0%	120.6 / NA	37	A89A (GCT→GCG)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/20);  total (17/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTAT‑CTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGC  >  CP006881/3724768‑3725036                                                                                                                                        |                                                                                                                                       tCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                                                                                     <  6:122128/138‑1 (MQ=255) tCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                                                                                     <  2:129167/138‑1 (MQ=255)      atcatcCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTCTAGATTTTCAAGAGGTTAT‑CTCTCCCGGCAATTTGCTTTTGACTGGCATTTTACCTCCTTTTGACCTCCATGTAAAGATGCGGGCCCGCtctctc                                                                                                                                <  8:298792/138‑1 (MQ=255)      atcatcCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCtctctc                                                                                                                                <  7:200576/138‑1 (MQ=255)      atcatcCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTcc                                                                                                                               >  5:248601/1‑139 (MQ=255)                tttATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                                                                                     <  1:136733/123‑1 (MQ=255)                tttATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                                                                                     >  2:136733/1‑123 (MQ=255)                  tattaAAGCCGCATTGATGTTTAAAACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCCTGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGa                                                                                                                    <  1:87425/138‑1 (MQ=255)                   attaAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGAt                                                                                                                   >  7:170421/1‑138 (MQ=255)                        aGCCGCATTGTTGTTTAATACTTTATAGAATTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCGATGTAAAGTTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTg                                                                                                             <  8:299902/139‑1 (MQ=255)                         gCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGCCTGGGATTTTCCATCCTTTAGACCGCCATGTAAAGATCCGGGCCCGCTCTCTCCTTCTTTGGCGATTCTTTg                                                                                                             >  7:153947/1‑138 (MQ=255)                         gCCGCATTGTTGATTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACGTCCTTTTGACCGACATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTg                                                                                                             >  4:140215/1‑138 (MQ=255)                             cATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGc                                                                                                        >  8:155547/1‑139 (MQ=255)                             cATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGc                                                                                                        >  3:181944/1‑139 (MQ=255)                             cATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGc                                                                                                        <  6:146440/139‑1 (MQ=255)                                  ttgttTAATACTTTATAGATTTTCAAGAGG‑TATGCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACAGCCATGTAAATATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCa                                                                                                   <  2:137538/139‑1 (MQ=255)                                    gttTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCaa                                                                                                  >  1:47710/1‑138 (MQ=255)                                       taatACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCTCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACa                                                                                               >  1:311721/4‑138 (MQ=255)                                            cTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACAtcttct                                                                                         >  1:5284/1‑139 (MQ=255)                                               tataGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTTCAAACATCTTCtttt                                                                                      >  6:328915/1‑139 (MQ=255)                                               tataGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCa                                                                                                   <  4:83389/126‑1 (MQ=255)                                               tataGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCa                                                                                                   >  3:83389/1‑126 (MQ=255)                                                    aTTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATaat                                                                                 >  8:295033/1‑138 (MQ=255)                                                      tttCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAgc                                                                               >  7:266139/1‑139 (MQ=255)                                                          aaGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCgcgg                                                                           <  2:47710/139‑1 (MQ=255)                                                          aaGAGGGAAT‑CTCTCCCGCCAATTGGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGcgcg                                                                            <  1:324611/138‑1 (MQ=255)                                                                         cccGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCtttc                                                             >  5:81992/1‑138 (MQ=255)                                                                                aTTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGctttt                                                      <  6:248601/139‑1 (MQ=255)                                                                                     cTTTTGACTGGGATTTTACCTCCTTTTGACCTCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTttcttc                                                  <  2:311721/138‑1 (MQ=255)                                                                                            cTGGGCTTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca                                          <  1:253592/139‑1 (MQ=255)                                                                                            cTGGGATTTTAGCTCCTTTTGACAGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca                                          <  8:153947/139‑1 (MQ=255)                                                                                            cTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca                                          <  5:183995/139‑1 (MQ=255)                                                                                               ggATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAg                                       >  5:346316/1‑139 (MQ=255)                                                                                                        ctcctTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGcc                              <  3:92246/139‑1 (MQ=255)                                                                                                                               aTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTCTAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAACACGAAACGAGTcct       <  8:170421/139‑2 (MQ=255)                                                                                                                                  cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTAAACAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg    <  4:181944/139‑1 (MQ=255)                                                                                                                                    ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTCGCTTTATGCGTTTATTCCGCATTAACAGCCCTCCCCCAATCATAAAACGAAACGAGTCCAGCGGc  >  2:4189/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       TCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTAT‑CTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGC  >  CP006881/3724768‑3725036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑