Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,932,408	G→A	5.4%	W139* (TGG→TAG)	U712_20270 →	Gluconokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,932,408	0	G	A	5.4%	233.5 / 2.3	75	W139* (TGG→TAG)	U712_20270	Gluconokinase
Reads supporting (aligned to +/- strand):  ref base G (40/29);  new base A (2/2);  total (42/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.32e-01

TACATGGGCCGACAACAGAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCATTGGCATCAGCGACAGGCATGATGAACCTG  >  CP006881/3932283‑3932544                                                                                                                              |                                                                                                                                         tACATGGGCCGACAACAGAAGTGAAGGCTGGGCGCATAGGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATga                                                                                                                              >  5:223357/1‑138 (MQ=255)  aCATGGGCCGACAACAGAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATga                                                                                                                              >  8:217289/1‑137 (MQ=255)  aCATGGGCCGACAACAGAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATga                                                                                                                              >  7:333996/1‑137 (MQ=255)      gggCCGACAACAGAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGACTGGATCACGATTgaaagaa                                                                                                                         >  8:144784/1‑138 (MQ=255)      gggCCGACAACAGAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATCAGGTGTACAAACGGACGGGAACACCGATTCATCCTATGGCACCGTTAAGTAAAATTGCATGGATCACGAAagaaagaa                                                                                                                         >  7:264665/1‑138 (MQ=255)        gCCGACAACAGAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATgaaagaa                                                                                                                         >  5:42868/1‑136 (MQ=255)        gCCGACAACAGAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATgaaagaa                                                                                                                         >  7:166032/1‑136 (MQ=255)        gCCGACAACAGAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATgaaagaa                                                                                                                         >  7:311633/1‑136 (MQ=255)        gCCGACAACAGAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTAGATCACGaa                                                                                                                                 <  4:176495/128‑1 (MQ=255)        gCCGACAACAGAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTAGATCACGaa                                                                                                                                 >  3:176495/1‑128 (MQ=255)             caacaGAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATc                                                                                                                 >  1:344620/1‑139 (MQ=255)                  gAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAAt                                                                                                                  >  4:76010/1‑133 (MQ=255)                  gAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAAt                                                                                                                  <  3:76010/133‑1 (MQ=255)                   aaGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTc                                                                                                            >  4:243279/1‑138 (MQ=255)                        aaGGCTGGGCGCATAAGATCAAAGAGGAATTGACCGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTAGATCACGAATGAAAGAAAAGAAATCGCTTCCAAgg                                                                                                       >  7:249195/1‑138 (MQ=255)                        aaGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAgg                                                                                                       >  2:5602/1‑138 (MQ=255)                        aaGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAgg                                                                                                       >  7:212111/1‑138 (MQ=255)                        aaGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATAGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAgg                                                                                                       >  7:35401/1‑138 (MQ=255)                               ggCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCaaaaaaa                                                                                               >  2:347594/1‑139 (MQ=255)                                gcgcATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCaaaaaaa                                                                                               >  8:159842/1‑138 (MQ=255)                                gcgcATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAat                                                                                              <  5:309229/139‑1 (MQ=255)                                                   aaTTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATa                                                                           <  7:321010/139‑1 (MQ=255)                                                   aaTTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATa                                                                           >  2:22469/1‑139 (MQ=255)                                                   aaTTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATa                                                                           >  7:70943/1‑139 (MQ=255)                                                   aaTTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATa                                                                           >  6:209690/1‑139 (MQ=255)                                                    aTTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATa                                                                           <  1:22469/138‑1 (MQ=255)                                                             aCATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTaaa                                                                 >  8:9795/1‑139 (MQ=255)                                                             aCATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTaaa                                                                 >  6:72513/1‑139 (MQ=255)                                                             aCATGACGCGTACAAACGTACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGGTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTaaa                                                                 <  7:99749/139‑1 (MQ=255)                                                                tGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAg                                                              <  7:9795/139‑1 (MQ=255)                                                                  aGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGc                                                             <  8:212111/138‑1 (MQ=255)                                                                  aGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTAGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGc                                                             <  8:249195/138‑1 (MQ=255)                                                                       tACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAatatat                                                                                          >  4:97568/1‑104 (MQ=255)                                                                       tACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAatatat                                                                                          <  3:97568/104‑1 (MQ=255)                                                                       tACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACAt                                                                        <  3:47846/122‑1 (MQ=255)                                                                       tACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTaaa                                                                 >  4:57214/1‑129 (MQ=255)                                                                       tACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTaaa                                                                 <  3:57214/129‑1 (MQ=255)                                                                       tACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTc                                                        >  5:75053/1‑138 (MQ=255)                                                                       tACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTc                                                        >  8:30112/1‑138 (MQ=255)                                                                       tACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTc                                                        >  6:305461/1‑138 (MQ=255)                                                                       tACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTc                                                        >  2:253751/1‑138 (MQ=255)                                                                       tACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTc                                                        >  8:287550/1‑138 (MQ=255)                                                                       tACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCa                                                       <  1:347594/139‑1 (MQ=255)                                                                       tACAAACGGACGGGAACACCGATTCATACGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACAt                                                                        >  4:47846/1‑122 (MQ=255)                                                                         cAACCGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAAt                                                     <  8:154617/139‑1 (MQ=255)                                                                         cAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAAt                                                     <  7:159842/139‑1 (MQ=255)                                                                         cAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAAt                                                     <  8:105596/139‑1 (MQ=255)                                                                         cAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAAt                                                     <  3:243279/139‑1 (MQ=255)                                                                          aaaCGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATg                                                    <  1:253751/139‑1 (MQ=255)                                                                            acggacggGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAAt                                                     >  7:155630/1‑136 (MQ=255)                                                                            acggacggGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAAt                                                     <  8:155630/136‑1 (MQ=255)                                                                             cggacggGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGt                                                 <  6:42868/139‑1 (MQ=255)                                                                             cggacggGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGt                                                 <  2:215247/139‑1 (MQ=255)                                                                             cggacggGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGt                                                 <  8:224138/139‑1 (MQ=255)                                                                              ggacggGAACACCGATTCATCCGATGGCCCCGTTAAGTAAAATTGCCTGGATCACGAAGGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCTTCAAAGAATACATTTTTAACCAGCTGTTCAATGAGTa                                                >  1:238823/1‑139 (MQ=255)                                                                                 cggGAACACCGATTCATCCGATGTCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATg                                              <  8:59051/138‑1 (MQ=255)                                                                                  gggAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAATGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTGCAATGAGTATGTc                                            <  1:5602/139‑1 (MQ=255)                                                                                          cGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATa                                                                           >  3:269848/1‑100 (MQ=255)                                                                                          cGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATa                                                                           <  4:269848/100‑1 (MQ=255)                                                                                            aTTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGa                                       <  5:131657/134‑1 (MQ=255)                                                                                            aTTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGa                                       >  6:131657/1‑134 (MQ=255)                                                                                               cATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCa                                <  5:72513/138‑1 (MQ=255)                                                                                                    gATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCATTGGCa                          >  5:313464/1‑139 (MQ=255)                                                                                                          aCCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCATTGGCATCAGc                     >  1:31353/1‑138 (MQ=255)                                                                                                              ttAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCATTGGCATCAGCGACAg                >  8:151198/1‑139 (MQ=255)                                                                                                              ttAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCATTGGCATCAGCGACAg                >  3:54656/1‑139 (MQ=255)                                                                                                              ttAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGAATACTCATTGGCATCAGCGACAg                >  4:243484/1‑139 (MQ=255)                                                                                                              ttAAGTAAAATCGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCATTGGCATCAGCGACAg                >  1:52097/1‑139 (MQ=255)                                                                                                                aaGTAAAATTGCCTTGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAATAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCATTTGCATCAGCGACAgg               <  3:97015/138‑1 (MQ=255)                                                                                                                     aaaTTGCCTGTACCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCATTGGCATCAGCGACAGGCatgat         <  1:166510/139‑1 (MQ=255)                                                                                                                     aaaTTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCATTGGCATCAGCGACAGGCatgat         <  7:151198/139‑1 (MQ=255)                                                                                                                     aaaTTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCATTGGCATCAGCGACAGGCatga          <  6:313464/138‑1 (MQ=255)                                                                                                                      aaTTGCCTTTATCTCGAATTAAAGAAATGAAATCGCTTCCAAGGCAAAAAAATATTTCGGCATCATAGAATACATTTTTAAACTGCTGTTCAATGAGTATGTCATCGATTACTCATTGGCATCAGCGACAGGCatgatg        <  8:35401/139‑1 (MQ=255)                                                                                                                           ccTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCATTGGCATCAGCGACAGGCATGATGAGCCt   >  1:110267/1‑139 (MQ=255)                                                                                                                            cTGTATCACTAATGTAAGAAAAGATATGGGTTACAACTCTATAATATATATGGGCATAAATTAATATATTTTTTATCTGGTGTTCAATGTGTTTGTCATGGTTTAGTCATTGGGATCAGGGACAGGCATGATGAACCTg  <  8:264665/139‑1 (MQ=255)                                                                                                                              |                                                                                                                                         TACATGGGCCGACAACAGAAGTGAAGGCTGGGCGCATAAGATCAAAGAGGAATTGAACGGACATGAGGTGTACAAACGGACGGGAACACCGATTCATCCGATGGCACCGTTAAGTAAAATTGCCTGGATCACGAATGAAAGAAAAGAAATCGCTTCCAAGGCAAAAAAATATATCGGCATCAAAGAATACATTTTTAAACAGCTGTTCAATGAGTATGTCATCGATTACTCATTGGCATCAGCGACAGGCATGATGAACCTG  >  CP006881/3932283‑3932544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑