BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,783	G→T	5.0%	intergenic (+68/‑88)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,783	0	G	T	5.0%	18079.7 / 124.6	5547	intergenic (+68/‑88)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base G (1645/3446); new base T (348/77); total (2012/3535)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5709 total aligned reads displayed.

TAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTT  >  pSIJ663_pHT315_araE_xy/3645‑3922

 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                             <  4:52138/138‑1 (MQ=255)

 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                             <  7:119768/138‑1 (MQ=255)

 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                             <  7:340449/138‑1 (MQ=255)

        aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGATTTTTTAAATTTTGAAAATTAAAAATTACAAAAGTTAATTGAGATAAATTATtatatataaa                                                                                                                                      >  6:338092/1‑136 (MQ=255)

         ttACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAAATTTGTAAGTTACACGGTAATAAAGTGAATTGAGATAAATTATtatatataaata                                                                                                                                    >  4:174859/1‑135 (MQ=255)

                tGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTGGTAATTTTGACGTTTCTTAATGGAATGGTGATAAATTATTTTATATAATAATGACAGc                                                                                                                             >  1:29339/1‑139 (MQ=255)

                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                        <  3:32314/139‑1 (MQ=255)

                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                        <  5:137964/139‑1 (MQ=255)

                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                        <  6:190679/139‑1 (MQ=255)

                     aaGTATTGTATATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                        <  8:195854/139‑1 (MQ=255)

                      aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                                                                       <  8:53048/139‑1 (MQ=255)

                      aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                                                                       <  8:11493/139‑1 (MQ=255)

                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                      <  5:274571/139‑1 (MQ=255)

                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCACTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                      <  7:88343/139‑1 (MQ=255)

                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATATTTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                                                     <  5:11325/138‑1 (MQ=255)

                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                                                     <  1:146691/138‑1 (MQ=255)

                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                                                     <  2:81560/138‑1 (MQ=255)

                          ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                                                                   <  5:163343/139‑1 (MQ=255)

                               tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                                                              <  1:243340/139‑1 (MQ=255)

                               tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAAATTTCACGTTACTAAAAGGGATGGAGAGAAATTATTATATATACTACAGACACCTTACAAGAAGCTaa                                                                                                               >  8:273315/1‑138 (MQ=255)

                                 tttttAATAGTTATCTATTATTTAACGGTAGTAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                                                                             <  7:164535/138‑1 (MQ=255)

                                 tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                                                              <  1:192936/137‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGATATAATTCTATGAGTCGCTTTTTTAAATTTTGAAAGTTACACGTTACTACAGGGAATGTAGATAAATTATTATATATACTACTGACAGCTTCCACGAAGCTAAagag                                                                                                           <  5:347163/138‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTCTGAGTCGCTTTTTTAAATTTGGAAAGATACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                           <  5:202426/138‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATAGTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                           <  1:12243/138‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                           <  6:128508/138‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                           <  4:108775/138‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                           <  5:97586/138‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                           <  5:75646/138‑1 (MQ=255)

                                           ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAATTTTAACGTTTCTAAAGGGGATGGAGATAAATTAATTTATATAATACTGACAGCTTCCAAGAAGCGAAATAGTTCCCTCgc                                                                                                  >  8:245308/1‑139 (MQ=255)

                                             aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGTAAGTTTAACGTTTCTAAAGGGAATGGTGATTAATTATTTGTTTTACTACTGAAAGCTTACAAGAAGCGAAAGAGGTCCCTGgccg                                                                                                >  6:313434/1‑137 (MQ=255)

                                                tattatTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTGTAAGTTTCACGTTTATAAAGGGAATGGTGTTTAATTTTTTGTTATAATACTGAAAGCGTCCAAGAAGCGAAATATGTGCCTAGGGccga                                                                                             >  8:323108/1‑137 (MQ=255)

                                                     tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTGGTAATTTTAAAGTTAATAAAAGGTATGGAGATAAATTTTTTTATATAATACTGACACACTACAAGAAGCAAAAAAAGTCCCTAACGCCGACaggg                                                                                        >  6:275321/1‑139 (MQ=255)

                                                     tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                                                         <  1:216446/138‑1 (MQ=255)

                                                               ggaAATAATTCTATGAGTCGCGTTTTTAAATTTTTTAATTTTAAAGTTAATTAATGGTATTTTGTAAAATTATTATATATTATAATGACAGCTTACAACAAGCTAAAGATGTCCCTAGCGCCGACGGGGAATTTggggg                                                                              >  2:25518/1‑135 (MQ=255)

                                                                  acagattctaTGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                           <  3:349668‑M1/135‑8 (MQ=255)

                                                                  aataatTCTATGAGTCGCTTTTTTAAATTTGGAAATTTACACGTTACTAAAGGGAATGGATATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                           <  3:25209‑M1/139‑8 (MQ=255)

                                                                  aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                           <  7:183862‑M1/139‑8 (MQ=255)

                                                                  aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTACCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                           <  3:227850‑M1/139‑8 (MQ=255)

                                                                  aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                           <  2:56279‑M1/139‑8 (MQ=255)

                                                                  aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                           <  4:147884‑M1/139‑8 (MQ=255)

                                                                  aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                           <  7:209092‑M1/139‑8 (MQ=255)

                                                                    taatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                                                                         <  3:158332‑M1/139‑10 (MQ=255)

                                                                      atTCTATGAGGAGCTTTTTTAAATTTGGAAAGTAACACGTTACTAAAGGGAATGGAGATATATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                                                                       <  6:219186‑M1/139‑12 (MQ=255)

                                                                         atttgtgtcgcttTTTTAAATTTCGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                                     <  1:69911‑M1/132‑14 (MQ=255)

                                                                            tGAGTCGCTTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                 <  1:263952‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                 <  5:325669‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                 <  4:280102‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                 <  7:51883‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                 <  7:27719‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                 <  3:76519‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGCTTTTTAAAAATTGGAAAGTTACACGTTACTAAAGGGAATGGAGAAAAATTATAACATAAACAACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                 <  8:143944‑M1/139‑18 (MQ=255)

                                                                             tagTCTCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGATTGGAGATAATTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                 <  1:100508‑M1/137‑18 (MQ=255)

                                                                              agccGCTGTTTTAAATTAGCAAATTAACACTGTACTAAACGGAATGGAGATAAATTATTAGATATACTCCTGACAGCTACCAACAAGCTAAAGAGGTCCCTAGCGCCTCCGTGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGaa                                                          <  4:34307/136‑1 (MQ=255)

                                                                              aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                               <  7:71568‑M1/139‑20 (MQ=255)

                                                                              aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                               <  6:96134‑M1/139‑20 (MQ=255)

                                                                              aGTCGATTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACCGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                               <  8:74103‑M1/139‑20 (MQ=255)

                                                                                     tttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGTATATATTTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTCGCGCCTAAGGGGAATTTGgggtacattgaaaaaggaagagtat                                                         <  5:248766‑M1/138‑26 (MQ=255)

                                                                                      ttttAAATTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                         <  2:198423‑M1/137‑26 (MQ=255)

                                                                                      ttttAAATTTGGAAAGTTACACGTTACTAAAGGGACTGGAGAAAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                         <  8:221289‑M1/137‑26 (MQ=255)

                                                                                      ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                         <  5:298386‑M1/137‑26 (MQ=255)

                                                                                      ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                         <  1:48494‑M1/137‑26 (MQ=255)

                                                                                          aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                                    <  3:55297‑M1/138‑31 (MQ=255)

                                                                                                         cccgtTACTAAATGGAATGGAGATAAATTAATAGCTATACTACTGACACCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                    <  5:226709‑M1/137‑47 (MQ=255)

                                                                                                         cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                    <  6:163238‑M1/139‑47 (MQ=255)

                                                                                                         cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                    <  8:297437‑M1/139‑47 (MQ=255)

                                                                                                           cGTTACAAAAGCGAATGGAGATAAATTATTAGATATACCACTGACAGCATCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcg                                  <  1:340515‑M1/139‑49 (MQ=255)

                                                                                                               aCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccc                               <  7:232088‑M1/138‑52 (MQ=255)

                                                                                                               aCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                 <  3:107358‑M1/104‑18 (MQ=255)

                                                                                                                cTAAAGGTAATGGAGATAAATTATTAGATATACTACTGCCAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                              <  5:53744‑M1/138‑53 (MQ=255)

                                                                                                                cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                              >  2:281886‑M1/1‑86 (MQ=255)

                                                                                                                cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                              >  8:343536‑M1/1‑86 (MQ=255)

                                                                                                                     gggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcagcatttccgtgtcgccgttattcc                        >  3:13405‑M1/1‑81 (MQ=255)

                                                                                                                        aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                     >  1:318259‑M1/1‑78 (MQ=255)

                                                                                                                        aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                     >  4:78141‑M1/1‑78 (MQ=255)

                                                                                                                        aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                     >  2:343120‑M1/1‑78 (MQ=255)

                                                                                                                        aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                     >  5:183695‑M1/1‑78 (MQ=255)

                                                                                                                        aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                     >  7:256385‑M1/1‑78 (MQ=255)

                                                                                                                        aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                     >  7:299666‑M1/1‑78 (MQ=255)

                                                                                                                        aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcactt                     >  3:301024‑M1/1‑78 (MQ=255)

                                                                                                                        aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtc                                   <  3:273144‑M1/125‑48 (MQ=255)

                                                                                                                        aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                               <  1:17154‑M1/97‑20 (MQ=255)

                                                                                                                        aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaagaagagtatgagtattcaacatttccgtgtcgcacttattccctt                     >  6:40599‑M1/1‑78 (MQ=255)

                                                                                                                          tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgataaaggaagagtatgagtattcaacatgtccgtgtcgcgcttattccctgtt                   >  2:227701‑M1/1‑76 (MQ=255)

                                                                                                                          tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                   >  2:1139‑M1/1‑76 (MQ=255)

                                                                                                                          tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                   >  2:314009‑M1/1‑76 (MQ=255)

                                                                                                                          tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                   >  6:67331‑M1/1‑76 (MQ=255)

                                                                                                                          tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                 <  8:168422‑M1/93‑18 (MQ=255)

                                                                                                                             agaTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttg                >  4:253734‑M1/1‑73 (MQ=255)

                                                                                                                             agaTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttg                >  7:146151‑M1/1‑73 (MQ=255)

                                                                                                                              gaTAAATTATTAGATATCCTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgc               <  7:318252‑M1/139‑68 (MQ=255)

                                                                                                                               aTAAATTATTAGATATACTCCTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTCCGGGGAATTTGgggtactttgaaaaaggaagcgtatgagtatacaacatttccttgtcgcccttattcccttttttgcg              >  6:256610‑M1/1‑71 (MQ=255)

                                                                                                                               aTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcg              >  3:203818‑M1/1‑71 (MQ=255)

                                                                                                                                 aaaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcgg             >  4:201972‑M1/1‑69 (MQ=255)

                                                                                                                                  aaatattagatATACTCCTCACAGCTCCCACGACGCTAAAGACGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggca           <  3:184631‑M1/136‑72 (MQ=255)

                                                                                                                                  aaatattagatATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggca           <  2:172184‑M1/136‑72 (MQ=255)

                                                                                                                                       ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                    <  1:89204‑M1/109‑47 (MQ=255)

                                                                                                                                       ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                 <  4:227728‑M1/80‑18 (MQ=255)

                                                                                                                                       ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                           <  2:144865‑M1/70‑8 (MQ=255)

                                                                                                                                         aGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgcc    >  4:309589‑M1/1‑61 (MQ=255)

TAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTT  >  pSIJ663_pHT315_araE_xy/3645‑3922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`