Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 193,074 T→A 8.8% intergenic (‑104/+6) U712_00870 ← / ← U712_00875 N‑acetylmuramic acid 6‑phosphate etherase/putative N‑acetyltransferase YbbJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881193,0740TA 8.8% 165.7 / 5.1 55intergenic (‑104/+6)U712_00870/U712_00875N‑acetylmuramic acid 6‑phosphate etherase/putative N‑acetyltransferase YbbJ
Reads supporting (aligned to +/- strand):  ref base T (32/18);  new base A (2/3);  total (34/21)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

TTGTAAGACGGTGAAGGTTTAATGGTTCTGACATGAGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGTTTTGCGCGTATGTTCAGATGATTGACGGCCAAG  >  CP006881/192937‑193203
                                                                                                                                         |                                                                                                                                 
ttGTAAGACGGTGAAGGTTTAATGGTTCTGACATGAGGATGCCCGCTGTTTTTAATTGATATATCTCAATATTCAGTATATTGTTTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACtt                                                                                                                                  <  3:301848/139‑1 (MQ=255)
ttGTAAGACGGTGAAGGTTTAATGGTTCTGACATGAGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACtt                                                                                                                                  <  6:236729/139‑1 (MQ=255)
          gTGAAGGTTTAATGGTTCTGACATGAGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATaa                                                                                                                        >  1:87324/1‑139 (MQ=255)
                  ttAATGGTTCTGACATGAGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAatgat                                                                                                                >  3:258525/1‑139 (MQ=255)
                    aaTGGTTCTGACATGAGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAatgatg                                                                                                               >  2:307708/1‑138 (MQ=255)
                     aTGGTTCTGACATGAGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAAtgatga                                                                                                              <  3:261720/138‑1 (MQ=255)
                     aTGGTGCTGACATGAGGATGCCCCCTGTTTTAAACTGATATATCTCAATAATCAGTATATTCATTTCTGTTATGATATATCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACATAAGACTATAAGAAAtgatga                                                                                                              <  6:73893/138‑1 (MQ=255)
                        gTTCTGACATGAGGATGCCCCCTGTTTTAAAATGATATATCTCATTATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAgg                                                                                                           <  3:119511/138‑1 (MQ=255)
                        gTTCTGACATGAGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAgg                                                                                                           <  6:272539/138‑1 (MQ=255)
                           cTGACATGAGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAATTGATGAAGGATc                                                                                                        >  2:128929/1‑138 (MQ=255)
                           cTGACATGAGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAATGATc                                                                                                        >  7:117093/1‑138 (MQ=255)
                              aCATGAGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACt                                                                                                    <  5:212646/139‑1 (MQ=255)
                                   aGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTtct                                                                                               >  7:319190/1‑139 (MQ=255)
                                   aGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTtct                                                                                               >  5:210569/1‑139 (MQ=255)
                                        gCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAg                                                                                          >  5:132914/1‑139 (MQ=255)
                                             cTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGcc                                                                                     <  3:24179/139‑1 (MQ=255)
                                              tGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGccc                                                                                    >  3:46397/1‑139 (MQ=255)
                                                   tAAAATGATATATCTAAATATTCAGTATATTCATTTCTGTTATTATATTTCAACTATTTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGACGGATCAACTGTTCTCCAAGCGGCCCGAtt                                                                                <  4:210079/138‑1 (MQ=255)
                                                          atatctCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAATACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGcc                                                                         <  8:183228/138‑1 (MQ=255)
                                                          atatatCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCg                                                                        >  2:109586/1‑139 (MQ=255)
                                                               tctcAATATTCATTATATTAATTTTTTTTATTATATTTCAACTTTGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCt                                                                    <  4:258525/138‑1 (MQ=255)
                                                               tctcAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCtt                                                                   >  7:47800/1‑139 (MQ=255)
                                                                   aaTATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTAt                                                                >  5:312825/1‑138 (MQ=255)
                                                                     tatTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATc                                                               >  6:94210/1‑137 (MQ=255)
                                                                          aGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAAc                                                        >  3:266657/1‑139 (MQ=255)
                                                                          aGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAAc                                                        >  3:199035/1‑139 (MQ=255)
                                                                            tataTTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAAccc                                                      >  4:107345/1‑139 (MQ=255)
                                                                                  cATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCttt                                                 <  3:107345/138‑1 (MQ=255)
                                                                                  cATTTCTGTTATGATATTTCAACAATTTTTTTATATTTTTGAATTTTTATTTCACTTAAGACTATAAGAATTGATGAAGCATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCttt                                                 <  6:210569/138‑1 (MQ=255)
                                                                                      tctcTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTATTCata                                            >  8:16283/1‑139 (MQ=255)
                                                                                      tCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGAtt                                                                                <  4:230091/103‑1 (MQ=255)
                                                                                      tCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGAtt                                                                                >  3:230091/1‑103 (MQ=255)
                                                                                      tCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATGAAGGAACCCGCTTTTCata                                            >  5:198587/1‑139 (MQ=255)
                                                                                      tCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCata                                            >  3:25078/1‑139 (MQ=255)
                                                                                      tCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCata                                            >  2:18481/1‑139 (MQ=255)
                                                                                      tCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTGTATCAAGGAACCCGCTTTTCat                                             >  2:141834/1‑138 (MQ=255)
                                                                                      tCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGACCTTTATCAAGGAACCCGCTTTTCata                                            >  5:331038/1‑139 (MQ=255)
                                                                                      tCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTAAAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATACTTCGCCGACCTTTATCAAGGAACCCGCTTTTCata                                            >  5:277511/1‑139 (MQ=255)
                                                                                       cTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCata                                            <  3:251673/138‑1 (MQ=255)
                                                                                          ttATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGTAACCCGCTTTTCAtata                                          >  6:255728/1‑137 (MQ=255)
                                                                                          ttATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCAtata                                          >  2:223084/1‑137 (MQ=255)
                                                                                            aTGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAg                                       <  6:132914/138‑1 (MQ=255)
                                                                                                  tttCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACATAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGtttt                                >  1:194693/1‑139 (MQ=255)
                                                                                                  tttCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACATAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGtttt                                >  1:199501/1‑139 (MQ=255)
                                                                                                    tCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCAtata                                          >  4:195825/1‑127 (MQ=255)
                                                                                                    tCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCAtata                                          <  3:195825/127‑1 (MQ=255)
                                                                                                    tCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACATAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGTTTTg                               <  2:199501/138‑1 (MQ=255)
                                                                                                    tCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACATAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGTTTTg                               <  2:194693/138‑1 (MQ=255)
                                                                                                      aaCTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGTTTTgcgc                            <  4:266657/139‑1 (MQ=255)
                                                                                                      aaCTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGTTTTgcgc                            <  1:307708/139‑1 (MQ=255)
                                                                                                           tGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGTTTTGCGCGTATg                       >  6:345730/1‑139 (MQ=255)
                                                                                                           tGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGTTTTGCGCGTATg                       >  8:77415/1‑139 (MQ=255)
                                                                                                           tGTTTTTAAATTTTTGAAATTTTATCTAACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCAGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGTTTTGCGCGTATg                       >  8:293115/1‑139 (MQ=255)
                                                                                                             tttttAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATAAAGGAACCCGCTTTTCATATAAAGGTGTTTTGCGCGTATGt                      >  5:244984/1‑138 (MQ=255)
                                                                                                               tttaaattttttaaatttTATTTAAATTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGTTTTGCGCGTATGTTc                    <  2:87324/138‑1 (MQ=255)
                                                                                                                ttAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGTTTTGCGCGTATGtt                     >  2:203193/1‑136 (MQ=255)
                                                                                                                                 tATTTAACTTATGAGTTTAAGAAATGATGATGGATAAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGTTTTGCGCGTATGTTCAGATGATTGACGGCCAAg  <  4:25078/138‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                 
TTGTAAGACGGTGAAGGTTTAATGGTTCTGACATGAGGATGCCCCCTGTTTTAAAATGATATATCTCAATATTCAGTATATTCATTTCTGTTATGATATTTCAACTATGTTTTTAAATTTTTGAAATTTTATTTCACTTAAGACTATAAGAAATGATGAAGGATCAACTGTTCTCCAAGCGGCCCGATTCTTCGCCGTCCTTTATCAAGGAACCCGCTTTTCATATAAAGGTGTTTTGCGCGTATGTTCAGATGATTGACGGCCAAG  >  CP006881/192937‑193203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑