Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,951,400 A→T 18.5% I224K (ATA→AAA)  U712_09660 ← D‑alanyl‑D‑alanine carboxypeptidase dacC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,951,4000AT18.5% 66.3 / 2.9 41I224K (ATA→AAA) U712_09660D‑alanyl‑D‑alanine carboxypeptidase dacC
Reads supporting (aligned to +/- strand):  ref base A (14/19);  new base T (6/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCAT  >  CP006881/1951273‑1951535
                                                                                                                                |                                                                                                                                        
cTTTGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTCTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGtt                                                                                                                               <  2:337407/139‑1 (MQ=255)
   tGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATAGGTTTTc                                                                                                                            >  2:60834/1‑139 (MQ=255)
        tGTTTGCGTCATCCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTT‑GGCTCGGTGTTTTTCGTTTTATAATCGGTTTTCggt                                                                                                                         >  1:14501/1‑136 (MQ=255)
              cGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGagacaca                                                                                                                 <  4:255016/139‑3 (MQ=255)
               gTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGagacagac                                                                                                                >  7:64008/1‑139 (MQ=255)
                     cGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCa                                                                                                          <  1:60834/139‑1 (MQ=255)
                        tACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATAGTTCGTTTTCGGTATATTATGGGTTTTGGGGGCGGCAGCCACgggcggc                                                                                                       >  4:113189/1‑134 (MQ=255)
                                         tAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTTTATAAACGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCtttttt                                                                                      >  7:100266/1‑138 (MQ=255)
                                         tAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCa                                                                                                          <  4:132695/119‑1 (MQ=255)
                                         tAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATAGGTTTTCGGAGAGACAGACACGGCa                                                                                                          >  3:132695/1‑119 (MQ=255)
                                           aTCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCttt                                                                                         <  6:127632/134‑1 (MQ=255)
                                           aTCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCttt                                                                                         >  5:127632/1‑134 (MQ=255)
                                           aTCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTT‑TCATGGTTGGTTTTGGTTTATTTATAGGGTTTGGGAGTGACAGCCACGGCCGGCTCTTCCCCGTGGTTTTGA‑t                                                                                   >  4:33045/1‑139 (MQ=255)
                                           aTCGGGTTTGTCCCATGCTCTCTTTCGTTGGTGAGGTCTTTCTCTGAACCGGCAGCAGGCGTTTTA‑GCAGCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑t                                                                                   <  8:64008/139‑1 (MQ=255)
                                              gtgtTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGGTT‑TCATAGTTGTTTTTAGGTTTTTATTAGGTTTTGGGTGAGACAGCCACGGCCGGCGGTTGCCCGTGGTTTTGG‑TTTg                                                                                >  4:223485/1‑139 (MQ=255)
                                                        cATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGGTCGTTATCGTTATATAATAGGTTTTCGGAGAGCCCGCCACGGCAGGCTGTTGCCCTTCTTTTTTA‑TTTTGGGTGGCCt                                                                       >  7:84636/1‑138 (MQ=255)
                                                         aTGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATAGTTATTTATCGTTATATAATCGGTTTTCGGAGAGGAAGACAACGCAGGCTCTTCCCCGGATTTTTTATTTTGGGGTgtgtat                                                                      >  7:94095/1‑134 (MQ=255)
                                                         aTGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTA‑GCCTCGGTATTTTTCGTTTTATAATGGGGTTTCGGGGAGACAGCCCCGGCCGGCGGGTCCCCGTCGTTTTGA‑TTTTGGGTGGccgc                                                                      >  6:60312/1‑136 (MQ=255)
                                                          tGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTT‑GGATCGTTCTTTATAGTTATATAATAGGTTTTAGGAGAGACAGACACGGCACGCTCTTACCCTTTGTTTTTA‑TTTTGGGTGTCCTcaa                                                                    >  7:241869/1‑139 (MQ=255)
                                                               tctTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTA‑GGATAGTTGGTTTTGGGTATTTAATAGGTTTTGGGGGTGACAGGCACCGCAGGCGCGTCCCCGGCTTTTTTA‑TTTGTGGTGGCCGCAACAAt                                                                >  6:22124/1‑138 (MQ=255)
                                                                   tCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATcaca                                                            <  3:311044/138‑1 (MQ=255)
                                                                      cttGGGAGGTCTTTCTCTGCACCGGCAGCAGGCGTTTTA‑GCATCGTTCTTTAGCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGc                                                        <  8:33183/138‑1 (MQ=255)
                                                                                       tgcagcGGCACCAGTCGTTTTA‑TCTTCTTTGATTATCTTTTTATAAACGTGTATCGGAGATACATACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGGGACCTCAACAATCACAGTGCCTGCATCATAGTCt                                          <  7:167743/131‑1 (MQ=255)
                                                                                         aaCCGGCAGCAGTCGTTTTA‑GCATCGTTCGTTATCGTTTTATAAAAGGTTTTAGGAGAGAAAGAAACGGCAGGCGCTTACCCGTCGTTTTGA‑TTTGGGGTGTCCTCAACCATCACAGAGGCGGCATCAAAGTATTAGtt                                     >  2:255665/1‑139 (MQ=255)
                                                                                             ggCAGCAGGAGTTTTA‑GCATCGTTCTTTATCTTTATATAATCGTTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGga                                 <  2:299931/139‑1 (MQ=255)
                                                                                               ccgcagTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGgaga                               <  8:100266/137‑1 (MQ=255)
                                                                                               cagcagT‑GTTTTATGCATCGTTCTTTATCGTTTTATAATCGGTTTGCGGAGACACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGgaga                               <  3:300639/139‑1 (MQ=255)
                                                                                                cgcagTCTTTTTA‑GCATCGTTCTTTATCTTTATATAATGGGTTTCCGGCCAGCCCCACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGAc                              <  5:46816/138‑1 (MQ=255)
                                                                                                   ctgcGTTTTA‑GCATGGTTCTTTTTCGTTAAATATTCGGTTTTCGGAGACACATCCACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGgaga                               <  2:78421/132‑1 (MQ=255)
                                                                                                     tCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGCCACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGt                         <  4:185793/139‑1 (MQ=255)
                                                                                                          ttA‑GCATCGTTCTTTATCGTTATTTAAGGGGTTTTCGGAGCCACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTaga                     <  5:85433/138‑1 (MQ=255)
                                                                                                                  cGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGctgtctc             >  1:78421/1‑133 (MQ=255)
                                                                                                                    ttCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTg           >  4:69508/1‑139 (MQ=255)
                                                                                                                    ttCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTATTCGTTTGGAGACGCTGTTAGATCGGAGATTg           >  7:201210/1‑139 (MQ=255)
                                                                                                                        ttATCTTTATATACTCGGTTTCCGCACACACCCCCACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGgcg        <  1:301219/138‑1 (MQ=255)
                                                                                                                         tATCGTTATATAATCGGTTTTCGGAGCGACAGCCACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGgcg        <  3:264998/137‑1 (MQ=255)
                                                                                                                         aaTCGTTATATAATCGGTTTTCGTAGAGACAGCCACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGgcg        <  6:5685/136‑1 (MQ=255)
                                                                                                                             gTTATATAATCGTTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCat  <  8:241869/139‑1 (MQ=255)
                                                                                                                              ttATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATcaca                                                            <  3:151236/80‑1 (MQ=255)
                                                                                                                              ttATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATcaca                                                            >  4:151236/1‑80 (MQ=255)
                                                                                                                              ttATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCat  >  8:61777/1‑138 (MQ=255)
                                                                                                                                |                                                                                                                                        
CTTTGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCAT  >  CP006881/1951273‑1951535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑