Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,951,425 A→C 28.1% S216A (TCT→GCT)  U712_09660 ← D‑alanyl‑D‑alanine carboxypeptidase dacC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,951,4250AC28.1% 37.1 / 2.6 40S216A (TCT→GCT) U712_09660D‑alanyl‑D‑alanine carboxypeptidase dacC
Reads supporting (aligned to +/- strand):  ref base A (11/17);  new base C (5/6);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCATAATATGTGTATTCATCGCTCCAA  >  CP006881/1951288‑1951558
                                                                                                                                          |                                                                                                                                      
gTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGagacagac                                                                                                                                       >  7:64008/1‑139 (MQ=255)
      cGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCa                                                                                                                                 <  1:60834/139‑1 (MQ=255)
         tACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATAGTTCGTTTTCGGTATATTATGGGTTTTGGGGGCGGCAGCCACgggcggc                                                                                                                              >  4:113189/1‑134 (MQ=255)
                          tAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTTTATAAACGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCtttttt                                                                                                             >  7:100266/1‑138 (MQ=255)
                          tAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCa                                                                                                                                 <  4:132695/119‑1 (MQ=255)
                          tAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATAGGTTTTCGGAGAGACAGACACGGCa                                                                                                                                 >  3:132695/1‑119 (MQ=255)
                            aTCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCttt                                                                                                                <  6:127632/134‑1 (MQ=255)
                            aTCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCttt                                                                                                                >  5:127632/1‑134 (MQ=255)
                            aTCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTT‑TCATGGTTGGTTTTGGTTTATTTATAGGGTTTGGGAGTGACAGCCACGGCCGGCTCTTCCCCGTGGTTTTGA‑t                                                                                                          >  4:33045/1‑139 (MQ=255)
                            aTCGGGTTTGTCCCATGCTCTCTTTCGTTGGTGAGGTCTTTCTCTGAACCGGCAGCAGGCGTTTTA‑GCAGCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑t                                                                                                          <  8:64008/139‑1 (MQ=255)
                               gtgtTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGGTT‑TCATAGTTGTTTTTAGGTTTTTATTAGGTTTTGGGTGAGACAGCCACGGCCGGCGGTTGCCCGTGGTTTTGG‑TTTg                                                                                                       >  4:223485/1‑139 (MQ=255)
                                         cATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGGTCGTTATCGTTATATAATAGGTTTTCGGAGAGCCCGCCACGGCAGGCTGTTGCCCTTCTTTTTTA‑TTTTGGGTGGCCt                                                                                              >  7:84636/1‑138 (MQ=255)
                                          aTGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATAGTTATTTATCGTTATATAATCGGTTTTCGGAGAGGAAGACAACGCAGGCTCTTCCCCGGATTTTTTATTTTGGGGTgtgtat                                                                                             >  7:94095/1‑134 (MQ=255)
                                          aTGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTA‑GCCTCGGTATTTTTCGTTTTATAATGGGGTTTCGGGGAGACAGCCCCGGCCGGCGGGTCCCCGTCGTTTTGA‑TTTTGGGTGGccgc                                                                                             >  6:60312/1‑136 (MQ=255)
                                           tGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTT‑GGATCGTTCTTTATAGTTATATAATAGGTTTTAGGAGAGACAGACACGGCACGCTCTTACCCTTTGTTTTTA‑TTTTGGGTGTCCTcaa                                                                                           >  7:241869/1‑139 (MQ=255)
                                                tctTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTA‑GGATAGTTGGTTTTGGGTATTTAATAGGTTTTGGGGGTGACAGGCACCGCAGGCGCGTCCCCGGCTTTTTTA‑TTTGTGGTGGCCGCAACAAt                                                                                       >  6:22124/1‑138 (MQ=255)
                                                    tCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATcaca                                                                                   <  3:311044/138‑1 (MQ=255)
                                                       cttGGGAGGTCTTTCTCTGCACCGGCAGCAGGCGTTTTA‑GCATCGTTCTTTAGCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGc                                                                               <  8:33183/138‑1 (MQ=255)
                                                                        tgcagcGGCACCAGTCGTTTTA‑TCTTCTTTGATTATCTTTTTATAAACGTGTATCGGAGATACATACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGGGACCTCAACAATCACAGTGCCTGCATCATAGTCt                                                                 <  7:167743/131‑1 (MQ=255)
                                                                          aaCCGGCAGCAGTCGTTTTA‑GCATCGTTCGTTATCGTTTTATAAAAGGTTTTAGGAGAGAAAGAAACGGCAGGCGCTTACCCGTCGTTTTGA‑TTTGGGGTGTCCTCAACCATCACAGAGGCGGCATCAAAGTATTAGtt                                                            >  2:255665/1‑139 (MQ=255)
                                                                              ggCAGCAGGAGTTTTA‑GCATCGTTCTTTATCTTTATATAATCGTTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGga                                                        <  2:299931/139‑1 (MQ=255)
                                                                                ccgcagTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGgaga                                                      <  8:100266/137‑1 (MQ=255)
                                                                                cagcagT‑GTTTTATGCATCGTTCTTTATCGTTTTATAATCGGTTTGCGGAGACACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGgaga                                                      <  3:300639/139‑1 (MQ=255)
                                                                                 cgcagTCTTTTTA‑GCATCGTTCTTTATCTTTATATAATGGGTTTCCGGCCAGCCCCACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGAc                                                     <  5:46816/138‑1 (MQ=255)
                                                                                    ctgcGTTTTA‑GCATGGTTCTTTTTCGTTAAATATTCGGTTTTCGGAGACACATCCACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGgaga                                                      <  2:78421/132‑1 (MQ=255)
                                                                                      tCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGCCACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGt                                                <  4:185793/139‑1 (MQ=255)
                                                                                           ttA‑GCATCGTTCTTTATCGTTATTTAAGGGGTTTTCGGAGCCACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTaga                                            <  5:85433/138‑1 (MQ=255)
                                                                                                   cGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGctgtctc                                    >  1:78421/1‑133 (MQ=255)
                                                                                                     ttCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTg                                  >  4:69508/1‑139 (MQ=255)
                                                                                                     ttCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTATTCGTTTGGAGACGCTGTTAGATCGGAGATTg                                  >  7:201210/1‑139 (MQ=255)
                                                                                                         ttATCTTTATATACTCGGTTTCCGCACACACCCCCACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGgcg                               <  1:301219/138‑1 (MQ=255)
                                                                                                          tATCGTTATATAATCGGTTTTCGGAGCGACAGCCACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGgcg                               <  3:264998/137‑1 (MQ=255)
                                                                                                          aaTCGTTATATAATCGGTTTTCGTAGAGACAGCCACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGgcg                               <  6:5685/136‑1 (MQ=255)
                                                                                                              gTTATATAATCGTTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCat                         <  8:241869/139‑1 (MQ=255)
                                                                                                               ttATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATcaca                                                                                   >  4:151236/1‑80 (MQ=255)
                                                                                                               ttATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATcaca                                                                                   <  3:151236/80‑1 (MQ=255)
                                                                                                               ttATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCat                         >  8:61777/1‑138 (MQ=255)
                                                                                                                    taATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCATAatat                    <  6:1172/138‑1 (MQ=255)
                                                                                                                              tCGGAGAGACAGCCACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCATAATATGTGTATTCAt          <  3:69508/138‑1 (MQ=255)
                                                                                                                              tCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCATAATATGTGTATTCAt          <  3:223485/138‑1 (MQ=255)
                                                                                                                              tCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCATAATATGTGTATTCAt          <  3:113189/138‑1 (MQ=255)
                                                                                                                                  ctagacacacACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCATAATATGTGTATTCATCGCTc     <  5:193016/137‑1 (MQ=255)
                                                                                                                                     gacacacaCGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCATAATATGTGTATTCATCGCTCCaa  <  1:255665/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
GTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTA‑GCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGA‑TTTGGGGTGACCTCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTGGCGCGCCATAATATGTGTATTCATCGCTCCAA  >  CP006881/1951288‑1951558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑