| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 2,061,119 | T→A | 6.8% | R915S (AGA→AGT) | U712_10210 ← | 2‑oxoglutarate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,061,119 | 0 | T | A | 6.8% | 179.9 / 4.5 | 59 | R915S (AGA→AGT) | U712_10210 | 2‑oxoglutarate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (26/29); new base A (2/2); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01 | |||||||||||
CTAATTCAGGTACCTTAATTTCCGCCATTTTTTCATTTCCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGCTCTTCCTGCACCCAAACGATTTCTTTCAGGTTCGGAAGTTTTGCA > CP006881/2060986‑2061254 | cTAATTCAGGTACCTTAATTTCCGCCATTTTTTCATTTCCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGtcttct < 1:239932/136‑1 (MQ=255)cTAATTCAGGTACCTTAATTTCCGCCATTTTTTCATTTCCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGtcttct < 5:63457/136‑1 (MQ=255)cTAATTCAGGTACCTTAATTTCCGCCATTTTTTCATTTCCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGtcttct < 3:276162/136‑1 (MQ=255) tAATTCAGGTACCTTAATATCCGCATTTTTTTCATTTCCCCCTTAGTTTTTGAGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTTCAGGGCTGGATCGTCTTCTTCGt < 6:175600/139‑1 (MQ=255) aTTCAGGTACCTTAATTTCCGCCATTTTTTCATTTCCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGt < 6:26564/137‑1 (MQ=255) ttCAGGTACCTTAATTTCCGCCATTTTTTCATTTCCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCa < 1:129181/139‑1 (MQ=255) ttCAGGTACCTTAATTTCCGCCATTTTTTCATTTCCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCa < 5:56929/139‑1 (MQ=255) ttCAGGTACCTTAATTTACGCCATTTTTTCATTTCCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCa < 8:138518/139‑1 (MQ=255) aGGTACCTTAATTTCCGCCATTTTTTCATTTCCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGAGCGTCTTCTTCGTCCAat < 3:134217/138‑1 (MQ=255) ccTTAATTTCCGCCATTTTTTCATTTCCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTg < 1:116225/139‑1 (MQ=255) cGCCTTTTTTTCATTTGCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACt < 6:114778/139‑1 (MQ=255) cGCCATTTTTTCATTTCCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACt < 8:261096/139‑1 (MQ=255) tttttGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAAt < 7:305847/139‑1 (MQ=255) cGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACggg < 4:170350/138‑1 (MQ=255) cGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACggg < 3:90230/138‑1 (MQ=255) cGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGc < 4:330135/139‑1 (MQ=255) cGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGc < 7:309496/139‑1 (MQ=255) cGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGc < 3:232750/139‑1 (MQ=255) cGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGc < 1:260981/139‑1 (MQ=255) cGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGc < 3:315738/139‑1 (MQ=255) aGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCa < 4:125875/138‑1 (MQ=255) gacaCAAAACGTTCCATGTCTTAATTATGACCTGTCGGATCTCCCTCAGCAGGGCTCGATCGCCTTCTTCGACCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAg < 6:283532/136‑1 (MQ=255) gATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTCATATAACCCCAAg < 5:89067/139‑1 (MQ=255) aCAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGccc > 3:72569/1‑139 (MQ=255) aaTACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGc > 4:146771/1‑119 (MQ=255) aaTACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGc < 3:146771/119‑1 (MQ=255) aaTACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGcccc > 1:154487/1‑138 (MQ=255) aaTACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTACCTCTGGGGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGcccc > 1:175682/1‑138 (MQ=255) aaTACGTTCCAGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGcccc > 6:7858/1‑138 (MQ=255) tACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGaa > 6:251292/1‑78 (MQ=255) tACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGaa < 5:251292/78‑1 (MQ=255) tACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCAt > 5:89379/1‑139 (MQ=255) tACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCAt > 4:97170/1‑139 (MQ=255) tACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTAAAATACGGGCTGAGATAACCCCAAGCCCCCag > 3:183294/1‑138 (MQ=255) tACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCGGTCAAATACGGGCTGATATAACCCCAAGCCCCCAt > 8:176031/1‑139 (MQ=255) tACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTACAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATTTAACCCCAACCCCCCAt > 7:326917/1‑139 (MQ=255) ttCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTc > 6:157990/1‑139 (MQ=255) tCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCt > 6:325575/1‑139 (MQ=255) tCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCACAAGCCCCCATGTTcc > 5:283532/1‑138 (MQ=255) tCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTACTTCGTCCAATATATTg < 4:98300/71‑1 (MQ=255) tCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTACTTCGTCCAATATATTg < 4:93201/71‑1 (MQ=255) tCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTACTTCGTCCAATATATTg > 3:93201/1‑71 (MQ=255) tCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTACTTCGTCCAATATATTg > 3:98300/1‑71 (MQ=255) tGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACggg < 1:316606/108‑1 (MQ=255) tGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACggg > 2:316606/1‑108 (MQ=255) cTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGct < 1:251155/138‑1 (MQ=255) cTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGct < 2:154487/138‑1 (MQ=255) cTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTACCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGct < 2:175682/138‑1 (MQ=255) ttttATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAAAGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATATCCCCAAGCCCCCATGTTCTGCGGCTCTTc > 1:26676/1‑139 (MQ=255) atttATGAACTGTCGTATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCAAATATTTTGAACCCTTCCTCCCTATGGTGAACTCTCTGACAAATACGCGCTGATATAACCCCAAGCCCACATGGTCTGCGGCTCTTc > 4:90709/2‑139 (MQ=255) tATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGCTCTTCCt < 4:198229/138‑1 (MQ=255) tCGGATCTCCCTCAGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGAGCAATCTCTGTCAAATACGGGCTGATATAACCCCCAGCCACCATGTTCTGCGGCTCTTCCTGCACCCaaa < 7:132015/138‑1 (MQ=255) ggCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGCTCTTCCTGCACCCAAACGATTTCTTTCAGGTTCg > 2:66441/1‑138 (MQ=255) ggCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGCTCTTCCTGCACCCAAACGATTTCTTTCAGGTTCg > 3:87783/1‑138 (MQ=255) ggCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGCTCTTCCTGCACCCAAACGATTTCTTTCAGGTTCg > 3:59716/1‑138 (MQ=255) gATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAg > 4:260180/1‑82 (MQ=255) gATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAg < 3:260180/82‑1 (MQ=255) gATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGCTCTTCCTGCACCCAAACGATTTCTTTCAGGTTCGGAAGtt > 7:6606/1‑139 (MQ=255) gATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGCTCTTCCTGCACCCAAACGATTTCTTTCAGGTTCGGAAGtt > 6:170082/1‑139 (MQ=255) gATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGCTCTTCCTGCACCCAAACGATTTCTTTCAGGTTCGGAAGtt > 6:57509/1‑139 (MQ=255) gATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGCTCTTCCTGCACCCAAACGAGTTCTTTCAGGTTCGGAAGtt > 1:54445/1‑139 (MQ=255) gATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGCTCTTCCTGCACCCAAACCATTTCTTTCAGGTTCGGAAGtt > 5:157636/1‑139 (MQ=255) tcttctTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGatat > 3:155802/1‑68 (MQ=255) tcttctTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGatat < 4:155802/68‑1 (MQ=255) tcttctTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACGCCAAGCCCCCATGTTCTGCGGCTCTTCCTGCACCCAAACGATTTCTTTCAGGTTCGGAAGTTTTGCa < 5:72344/139‑1 (MQ=255) tcttctTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGc < 1:288527/94‑1 (MQ=255) tcttctTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGc > 2:288527/1‑94 (MQ=255) tcttctTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGCTCTTCCTGCACCCAAACGATTTCTTTCAGGTTCGGAAGTTTTGc > 5:91682/1‑138 (MQ=255) | CTAATTCAGGTACCTTAATTTCCGCCATTTTTTCATTTCCCCCTTAGTTTTTGCGAGTCAAGCTATCAGATACAATACGTTCCTGTTCTTTTTTATGAACTGTCGGATCTCCCTCTGCAGGGCTGGATCGTCTTCTTCGTCCAATATATTGAACGCTTACTCCCTCTGGTGCAATCTCTGTCAAATACGGGCTGATATAACCCCAAGCCCCCATGTTCTGCGGCTCTTCCTGCACCCAAACGATTTCTTTCAGGTTCGGAAGTTTTGCA > CP006881/2060986‑2061254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |