Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	195,500	G→A	5.8%	E24K (GAA→AAA)	U712_00890 →	Anti‑sigma‑W factor rsiW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	195,500	0	G	A	5.8%	223.0 / 2.2	69	E24K (GAA→AAA)	U712_00890	Anti‑sigma‑W factor rsiW
Reads supporting (aligned to +/- strand):  ref base G (38/27);  new base A (2/2);  total (40/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

GCGGATTCACAGAGGCAGAGAGGCTCTTAGAAAACAATTAAGGGATCTTTAAGTGGGGTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGCCCCCGCGGATTTTACCGCTAATGTCAT  >  CP006881/195366‑195633                                                                                                                                       |                                                                                                                                      gcgGATTCACAGAGGCAGAGAGGCTCTTAGAAAACAATTAAGGGATCTTTAAGTGGGGTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAaca                                                                                                                                   <  8:153040/139‑1 (MQ=255) gcgGATTCACAGAGGCAGAGAGGCTCTTAGAAAACAATTAAGGGATCTTTAAGTGGGGTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAaca                                                                                                                                   <  3:30811/139‑1 (MQ=255)    gATTCACAGAGGCAGAGAGGCTCTTAGAAAACAATTAAGGGATCTTTAAGTGGGGTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGt                                                                                                                                <  2:178765/139‑1 (MQ=255)            gagGCAGAGAGGCTCTTAGAAAACAATTAAGGGATCTTTAAGTGGGGTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATg                                                                                                                        <  3:132682/139‑1 (MQ=255)              ggCAGAGAGGCTCTTAGAAAACAATTAAGGGATCTTTAAGTGGGGTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGaa                                                                                                                      <  4:350443/139‑1 (MQ=255)               gCAGAGAGGCTCTTAGAAAACAATTAAGGGATCTTTAAGTGGGGTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAAc                                                                                                                     <  3:176175/139‑1 (MQ=255)                     agGCTCTTAGAAAACAATTAAGGGATCTTTAAGTGGGGTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTg                                                                                                                >  3:131709/1‑138 (MQ=255)                             aGAAAACAATTAAGGGATCTTTAAGTGGGGTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGc                                                                                                       >  3:231809/1‑139 (MQ=255)                                                    aGTGGGGTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCAttttt                                                                                 <  3:101233/138‑1 (MQ=255)                                                        gggTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTAc                                                                               >  7:260451/1‑136 (MQ=255)                                                        gggTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTAc                                                                               >  1:93995/1‑136 (MQ=255)                                                        gggTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATAAGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTAc                                                                               >  5:184503/1‑136 (MQ=255)                                                         ggTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTAc                                                                               >  3:284471/1‑135 (MQ=255)                                                          gTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATg                                                                                                                        >  5:335082/1‑93 (MQ=255)                                                          gTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATg                                                                                                                        <  6:335082/93‑1 (MQ=255)                                                          gTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGAt                                                                          >  4:68869/1‑139 (MQ=255)                                                             atgaAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGga                                                                       <  5:280998/139‑1 (MQ=255)                                                                        tGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAg                                                            <  4:295789/139‑1 (MQ=255)                                                                          tCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGga                                                                       >  4:140822/1‑126 (MQ=255)                                                                          tCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGga                                                                       <  3:140822/126‑1 (MQ=255)                                                                               aaCAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTc                                                       <  2:93995/137‑1 (MQ=255)                                                                                aCAATTTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTa                                                    <  6:301069/139‑1 (MQ=255)                                                                                aCAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGt                                                     <  4:284471/138‑1 (MQ=255)                                                                                    aTTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCTTACg                                                  >  2:28040/1‑137 (MQ=255)                                                                                    aTTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACg                                                  >  3:83268/1‑137 (MQ=255)                                                                                    aTTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACg                                                  >  7:4230/1‑137 (MQ=255)                                                                                    aTTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATAAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACg                                                  >  4:58516/1‑137 (MQ=255)                                                                                    aTTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATAAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACg                                                  >  4:63404/1‑137 (MQ=255)                                                                                     ttGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAg                                               <  3:136749/139‑1 (MQ=255)                                                                                     ttGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAg                                               <  8:190621/139‑1 (MQ=255)                                                                                       gtgCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGc                                              <  2:8968/138‑1 (MQ=255)                                                                                        tgCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGca                                             <  4:152098/138‑1 (MQ=255)                                                                                             cgtaagcatatgcatCTTTATGGACATACCCTTCCAAAAGCTGAACAAGTACTAAATGAACCTCTCGAGACATGCGAGAACTGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCGCGTACGGAGCACATCGc                                       <  5:216994/134‑1 (MQ=255)                                                                                               tatgcatatgcaTCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCAt                                     >  2:223794/1‑139 (MQ=255)                                                                                                      atgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGaa                                                                    >  7:44720/1‑101 (MQ=255)                                                                                                      atgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGaa                                                                    <  8:44720/101‑1 (MQ=255)                                                                                                       ttcatcatGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACAAGCGAGAAATGCAGAAAGCATTTTTACGAGCTTGAGTAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAg                              <  1:29093/136‑1 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACTCGTATTAAATGATCATCTGGAGACATGCGAGAAATGCAGAAAGCTTTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCCCATCGCATGTCGAAGc                             >  7:99681/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAgcgc                                                         >  2:122933/1‑111 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAgcgc                                                         <  1:122933/111‑1 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATATCTCTCTTACGGAGCACATCGCATGTCGAAGc                             >  3:140032/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGc                                       >  4:121915/1‑129 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGc                                       <  3:121915/129‑1 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAg                              <  4:131709/138‑1 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  6:6691/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  6:54940/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  5:179157/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  6:157375/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  7:310920/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  4:67959/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  3:54796/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  3:51313/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  3:272936/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  2:35045/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  8:297945/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  1:4984/1‑139 (MQ=255)                                                                                                       tgcatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGc                             >  1:309164/1‑139 (MQ=255)                                                                                                       tgaatCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATCCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGCAGCACATCGCATGTCGAAGc                             >  3:33796/4‑139 (MQ=255)                                                                                                               gATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGCCCCcg                        <  7:297945/136‑1 (MQ=255)                                                                                                                     gaTATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGCCCCCGCGGATTTTa               <  2:4984/139‑1 (MQ=255)                                                                                                                      atatCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGCCCCCGCGGATTTTAc              >  1:342620/1‑139 (MQ=255)                                                                                                                        atCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGgag                                                                      <  1:197312/81‑1 (MQ=255)                                                                                                                        atCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGgag                                                                      >  2:197312/1‑81 (MQ=255)                                                                                                                          ccTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGCCCCCGCGGATTTTACCGc           >  3:205489/1‑138 (MQ=255)                                                                                                                              ccAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGCCCCCGCGGAGTTTCCAGCTAATg      >  2:105344/1‑139 (MQ=255)                                                                                                                                aaaaGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGCCCCCGCGGATTTTACCGCTAATGt     >  4:313343/1‑138 (MQ=255)                                                                                                                                 aaaGATAAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGCCCCCGCGGATTTTACCGCTAATGTCa   <  3:63404/139‑1 (MQ=255)                                                                                                                                 aaaGATAAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGCCCCCGCGGATTTTACCGCTAATGTCa   <  3:58516/139‑1 (MQ=255)                                                                                                                                  aaGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGCCCCCGCGGATTTTACCGCTAATGTCAt  >  5:151931/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                      GCGGATTCACAGAGGCAGAGAGGCTCTTAGAAAACAATTAAGGGATCTTTAAGTGGGGTGATGAAATGAGCTGTCCTGAACAAATTGTGCAGCTTATGCATATGCATCTTGATGGAGATATCCTTCCAAAAGATGAACACGTATTAAATGAACATCTGGAGACATGCGAGAAATGCAGAAAGCATTTTTACGAGATGGAGAAATCCATAGCGCTCGTACGGAGCACATCGCATGTCGAAGCCCCCGCGGATTTTACCGCTAATGTCAT  >  CP006881/195366‑195633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑