Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,179,036 T→G 7.7% A185A (GCT→GCG)  U712_10905 → UPF0750 membrane protein ypjC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,179,0360TG 7.7% 192.4 / 6.6 65A185A (GCT→GCG) U712_10905UPF0750 membrane protein ypjC
Reads supporting (aligned to +/- strand):  ref base T (38/21);  new base G (3/2);  total (42/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

GTTTGGTCAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCATTTTGAAAGGACAGG  >  CP006881/2178900‑2179172
                                                                                                                                        |                                                                                                                                        
gTTTGGTCAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGc                                                                                                                                        <  4:226925/139‑1 (MQ=255)
                       aTTCCGATGGGCAGAACGATGTTTGCTTGCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAgg                                                                                                                 <  3:336342/139‑1 (MQ=255)
                        ttCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGGGGCAAGATTAATCGATTTTATTCAgg                                                                                                                 >  2:319607/1‑138 (MQ=255)
                        ttCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAgg                                                                                                                 >  5:117917/1‑138 (MQ=255)
                        ttCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAgg                                                                                                                 >  3:264023/1‑138 (MQ=255)
                        ttCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAgg                                                                                                                 >  1:221486/1‑138 (MQ=255)
                                     aaCGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATTCCCTGGTGGCTGTTTTTGTAGCTGCAAGATTTATTGATTTTATTGAGGAAGGCGGATACgct                                                                                                   >  1:305599/1‑139 (MQ=255)
                                     aaCGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACgct                                                                                                   >  1:108110/1‑139 (MQ=255)
                                     aaCGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACgct                                                                                                   >  7:19033/1‑139 (MQ=255)
                                          tGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGAtt                                                                                                                          <  1:330633/111‑1 (MQ=255)
                                          tGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGAtt                                                                                                                          >  2:330633/1‑111 (MQ=255)
                                                   tCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACgctgct                                                                                                >  1:42944/1‑128 (MQ=255)
                                                   tCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACgctgct                                                                                                <  2:42944/128‑1 (MQ=255)
                                                      aTGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGc                                                                                                            >  1:186835/1‑113 (MQ=255)
                                                      aTGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGc                                                                                                            <  2:186835/113‑1 (MQ=255)
                                                                  tcatTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGa                                                                       >  5:288379/1‑138 (MQ=255)
                                                                  tcatTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGa                                                                       >  3:126404/1‑138 (MQ=255)
                                                                             ttGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTCTATTTGTAGCTGCAAGATTAATATATTTTATTAAGGAAGTCTGAAACGCTGCTAAAGGCGCTACGATTATCTATTCGAAAAACGAGCTGa                                                           >  3:192374/1‑139 (MQ=255)
                                                                              tGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGAt                                                               <  2:288807/134‑1 (MQ=255)
                                                                              tGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGAt                                                               >  1:288807/1‑134 (MQ=255)
                                                                                       aTCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGaaa                                                   <  1:207998/137‑1 (MQ=255)
                                                                                        tctctcCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGAtt                                                                                                                          <  3:262069/65‑1 (MQ=255)
                                                                                        tctctcCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGAtt                                                                                                                          >  4:262069/1‑65 (MQ=255)
                                                                                               tACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAATATTCtt                                          >  6:324069/1‑138 (MQ=255)
                                                                                               tACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCtt                                          >  3:318221/1‑138 (MQ=255)
                                                                                                 cAAAGAAGCGATGTATACCCTTGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGCAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTg                                         <  4:248359/137‑1 (MQ=255)
                                                                                                 cAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTgaa                                       >  5:12411/1‑139 (MQ=255)
                                                                                                      aagCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAAt                                  >  5:251789/1‑139 (MQ=255)
                                                                                                      aagCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAAt                                  >  6:150788/1‑139 (MQ=255)
                                                                                                      aagCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAAt                                  >  7:137743/1‑139 (MQ=255)
                                                                                                      aagCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAAt                                  >  2:204818/1‑139 (MQ=255)
                                                                                                         cGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATgg                                >  4:104191/1‑138 (MQ=255)
                                                                                                           aTGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGaaaaa                                                                   >  8:269374/1‑101 (MQ=255)
                                                                                                           aTGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGaaaaa                                                                   <  5:237641/101‑1 (MQ=255)
                                                                                                           aTGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGaaaaa                                                                   >  6:237641/1‑101 (MQ=255)
                                                                                                           aTGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGaaaaa                                                                   <  7:269374/101‑1 (MQ=255)
                                                                                                           aTGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAAc                             <  6:251789/139‑1 (MQ=255)
                                                                                                                taCCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGc                        >  3:263545/1‑139 (MQ=255)
                                                                                                                taCCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGc                        >  8:115138/1‑139 (MQ=255)
                                                                                                                   ccTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGAGGATACGCTGCTAAAGGCGCTATGTTTATCTCTTCGAAATACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAgtgtt                      <  4:264023/138‑5 (MQ=255)
                                                                                                                      ggtggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACg                                                                                                     >  3:211497/1‑56 (MQ=255)
                                                                                                                      ggtggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACg                                                                                                     <  4:211497/56‑1 (MQ=255)
                                                                                                                      ggtggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGa                           <  1:16620/130‑1 (MQ=255)
                                                                                                                      ggtggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGa                           >  2:16620/1‑130 (MQ=255)
                                                                                                                      ggtggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCAc                   >  5:53262/1‑138 (MQ=255)
                                                                                                                      ggtggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCAc                   >  6:183856/1‑138 (MQ=255)
                                                                                                                      ggtggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCAc                   >  7:192460/1‑138 (MQ=255)
                                                                                                                      ggtggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCAc                   >  6:254451/1‑138 (MQ=255)
                                                                                                                       gtggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCa                 >  2:24161/1‑139 (MQ=255)
                                                                                                                        ttgatGTATTTGTAGCTTAAAGATTAATTGATTTTATTCAGGAAGGCTGATACTCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCAt                <  4:263545/135‑1 (MQ=255)
                                                                                                                        tggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGgcg                                                                                         >  8:250864/1‑66 (MQ=255)
                                                                                                                        tggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGgcg                                                                                         <  7:250864/66‑1 (MQ=255)
                                                                                                                        tggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCAt                >  8:341154/1‑139 (MQ=255)
                                                                                                                        tggCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCAt                >  4:53224/1‑139 (MQ=255)
                                                                                                                          gCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCAttt              >  2:91097/1‑139 (MQ=255)
                                                                                                                            tGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTTAAGAAATGGAACGAGGCGTGAcccattt             >  1:177271/1‑133 (MQ=255)
                                                                                                                             gTATTTGTACCAGCAAGAGTAATTGATGTTAGTCAGGACGGCGGATACGCTGCGAAAGGCGCTCCGAGTATCTCTTCGAAAAACGATCTGAGTCAGAAAAAGATTCTACAAGAAATGGAACGAGGCGTCACCAGTTTg            >  2:224741/1‑138 (MQ=255)
                                                                                                                              tATTTGTAGCTGCAAGATTAATTGATTATATTCAGGAAGGCGGATACGCTGCTAACCGCGCTACGAATATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCATTTTGa           <  2:305599/138‑1 (MQ=255)
                                                                                                                                   gTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCATTTTGAAAGGa      <  4:318221/138‑1 (MQ=255)
                                                                                                                                    tAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTAt                                                                               >  2:66297/1‑64 (MQ=255)
                                                                                                                                    tAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTAt                                                                               <  1:66297/64‑1 (MQ=255)
                                                                                                                                      gCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTACAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCATTTTGAAAGGACAg   <  6:12411/138‑1 (MQ=255)
                                                                                                                                      gCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCATTTTGAAAGGACAgg  >  3:310414/1‑139 (MQ=255)
                                                                                                                                      gCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCATTTTGAAAGGACAg   <  7:115138/138‑1 (MQ=255)
                                                                                                                                      gCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCATTTTGAAAGGACAg   <  2:184446/138‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GTTTGGTCAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCATTTTGAAAGGACAGG  >  CP006881/2178900‑2179172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑