| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 2,289,100 | G→T | 100% | P184T (CCG→ACG) | U712_11585 ← | putative oxidoreductase yqjQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,289,100 | 0 | G | T | 96.3% | 72.9 / ‑5.1 | 26 | P184T (CCG→ACG) | U712_11585 | putative oxidoreductase yqjQ |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (11/14); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGGGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTCCCCGCTTGAGAGG > CP006881/2288966‑2289237 | gATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGccc > 5:286490/1‑139 (MQ=255)gATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGcc < 1:103652/138‑1 (MQ=255)gATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGcc < 3:69308/138‑1 (MQ=255)gATCTCCCGCTTTTTCGTAAAAATTGCAGCTGAAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGcc < 5:133498/138‑1 (MQ=255) ccGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGG‑AAAAAAGTCCGTCTGAATCGTGCCCgg < 2:144852/134‑1 (MQ=255) cAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAACTGcc > 8:222590/1‑139 (MQ=255) tGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTc > 4:280361/1‑138 (MQ=255) ccACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTcc < 4:299377/137‑1 (MQ=255) tcatcaGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCTATCCGCa < 3:280361/139‑1 (MQ=255) tCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTcc < 1:216702/123‑1 (MQ=255) tCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTcc > 2:216702/1‑123 (MQ=255) aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg < 6:145656/139‑1 (MQ=255) aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGGGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg < 8:42032/139‑1 (MQ=255) ccAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAAcc > 8:68107/1‑138 (MQ=255) caccGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATAAACAATATAAAAAAAAAAAGTCTGAATAATTCCCGCGTTGACTGTTGTCACATAAATGCCGGTTACCGAAATCTACAACCGCAAAGCGTTTGAGTAACCt > 8:37456/4‑138 (MQ=255) cAGCGGCCGACATTTTTGGGTTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATAGTGCCCCGGTTGCCTGTTGTCACATAAAAGCCCGTTCCCGAAAGCTCCATCCGCAAAGCGATTCTGTAACCt > 2:217165/1‑138 (MQ=255) gCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAATAAAAGGCCGGCGGCATGGTGCCCGGGTTGACTGTTGTCCCATAAATGCCGGGTCCCGCAAGCTCCATCCGCAAGGCGTTTGTGTAACCTAACACg > 6:258930/1‑139 (MQ=255) cGACTTTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGGCGGTCTGAATCGTGCCCGGGTTGACTGTTTTGACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTATCCTAACACGGc < 7:56494/139‑1 (MQ=255) gCGTAGTCCCGGCCTTTATCAGCAATGTAAAAATCGTCCGTCGGAATCGTGCCCGGGTTGACTGTTGGCAAATAAATGCCGGTGCCCTAAAGCTCCATCCGCAAAGCGTTTGGGTAACCTAACACGGCATGTTTGGTcg < 1:217165/139‑1 (MQ=255) ccccGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCGCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATa < 3:31136/139‑1 (MQ=255) ccccGCCTTTATCAGCAATGGAAAAAAAGTCCGGCTGAATCGTGCCCGGGGTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATa < 7:153391/139‑1 (MQ=255) cccGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATa < 7:68107/138‑1 (MQ=255) cGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAg < 7:37456/139‑1 (MQ=255) cAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCgg > 3:17182/1‑139 (MQ=255) aaTGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTACCCTAACCCGGCAGGTTTGGTCGCGGAATACAGGCTAGACTTCGgt > 2:274760/1‑139 (MQ=255) gAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTgg > 2:29641/1‑139 (MQ=255) gggCCCGGGTGTACTGGTGTCACATAAATGCCGGTCACCGAAAGCGCCATCGGAAAAGCGTTTGAGTAACCTACCACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTCCCCGCTTGAGAgg < 4:17182/139‑1 (MQ=255) | GATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGGGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTCCCCGCTTGAGAGG > CP006881/2288966‑2289237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |