| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 230,497 | A→G | 11.0% | H323R (CAC→CGC) | U712_01065 → | Fatty‑acid peroxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 230,497 | 0 | A | G | 11.0% | 88.3 / 5.1 | 38 | H323R (CAC→CGC) | U712_01065 | Fatty‑acid peroxygenase |
| Reads supporting (aligned to +/- strand): ref base A (22/10); new base G (2/2); total (25/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01 | |||||||||||
TGTTTGTGCAGGAGGTCCGCAGATATTATCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAGGGGAAGGCATTA > CP006881/230362‑230634 | tGTTTGTGCAGGAGGTCCGCAGATATTATCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTTAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACgc > 8:78224/1‑138 (MQ=255) tgtgCAGGAGGTCCGCAGATATTATCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCt > 5:72832/1‑139 (MQ=255) ttatCCGTTCGGCCCGTTTTTAGGGGCGCTTGGCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGACCCACGACCCTCGTCTATGGGATCATCCCGAt > 2:88160/1‑139 (MQ=255) ttatCCGTTCGGCCCGTTTTTAGGGGCGATTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGACGGGCACAGCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGAt < 7:228574/139‑1 (MQ=255) cGTTCGGCCCGTTTTTCGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGACCGACCCACGACCCTCGTCTATGGGATCATCCCGATGAAtt < 3:86373/139‑1 (MQ=255) cgcTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCg > 5:177292/1‑137 (MQ=255) tGTTTGCAAAAACTTTGATTTTAAGACGGGCACATGGGTGCTGCTTTATTTATATGCAACCACCCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGtt < 1:237246/138‑1 (MQ=255) gAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGatat > 6:295788/1‑138 (MQ=255) aaTAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGCGCGGGAAGAAAATCTGTTTGATATGa > 7:297015/1‑139 (MQ=255) aaTAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGa < 6:72832/139‑1 (MQ=255) aGTTTAAGAAGGGCACATCGGTGTTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAgg > 7:181386/1‑139 (MQ=255) aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCGCGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCg > 3:314433/1‑106 (MQ=255) aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCGCGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCg > 3:319398/1‑106 (MQ=255) aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCGCGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCg < 4:314433/106‑1 (MQ=255) aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCGCGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCg < 4:319398/106‑1 (MQ=255) aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAgg > 2:212153/1‑139 (MQ=255) aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAgg > 6:146286/1‑139 (MQ=255) agaagGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCggggg > 3:337322/1‑139 (MQ=255) gggCACATCGGTGCTGCTTGATTTATATGGAACGAACCCCTACCCTCGTCTATGGGATCATCACGAGTAATTCCGGCCGGAACGATTTGCGGAGAGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCAc > 6:137829/1‑139 (MQ=255) ggCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACg > 2:318441/1‑139 (MQ=255) ggCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACg > 4:261583/1‑139 (MQ=255) cacaTCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACTcc > 1:199574/1‑139 (MQ=255) ccGGTGCGGCTTGATTATTTTGCACCCCCCCCCGCCCCTCGTCTATGGGAGCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCgaga < 4:307957/138‑1 (MQ=255) ctgctTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCg > 8:87427/1‑83 (MQ=255) ctgctTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCg < 7:87427/83‑1 (MQ=255) ctTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCAcc > 6:313139/1‑139 (MQ=255) gATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCg < 5:146286/137‑1 (MQ=255) tttATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGt > 1:330292/1‑139 (MQ=255) tataTGGAACGACCCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTc < 1:212153/138‑1 (MQ=255) atGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCACGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAgg > 4:258428/1‑139 (MQ=255) atGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAgg > 6:114423/1‑139 (MQ=255) atGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAgg > 7:94923/1‑139 (MQ=255) aacgaacCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAgggg > 4:139399/1‑137 (MQ=255) aacgaacCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAgggg > 7:60290/1‑137 (MQ=255) acgaacCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAGGGGa < 8:60290/137‑1 (MQ=255) cgaacCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAggg > 2:60433/1‑134 (MQ=255) cgaacCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAggg < 1:60433/134‑1 (MQ=255) cACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAGGGGAAGGCATTa < 8:297015/139‑1 (MQ=255) | TGTTTGTGCAGGAGGTCCGCAGATATTATCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAGGGGAAGGCATTA > CP006881/230362‑230634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |