Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,928,823 A→C 17.2% F124V (TTT→GTT)  U712_15080 ← Bacitracin export permease protein BceB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,928,8230AC17.2% 73.3 / 3.4 39F124V (TTT→GTT) U712_15080Bacitracin export permease protein BceB
Reads supporting (aligned to +/- strand):  ref base A (17/15);  new base C (3/4);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCATGATCAAAAGGTAAATGCCGCAGAACACAATGACCGTTTGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACAGCCCGATTTCTTTACTCCGTCTCTTAATAAAAA  >  CP006881/2928686‑2928960
                                                                                                                                           |                                                                                                                                         
gatcatgatcaAAAGGTAAATGCCGCAGAACACAATGACCGTTTGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAAc                                                                                                                                          >  1:35860/1‑139 (MQ=255)
     tgatcaAAAGGTAAATGCCGCAGAACACAATGACCGTTTGGACCAAGGACTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGATCAGAAT‑CATCAAGACAAGCTTCGATATCGAAACCCAGGc                                                                                                                                     >  7:88214/1‑139 (MQ=255)
      gatcaAAAGGTAAATGCCGCAGAACACAATGACCGTTTGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAACCCCGGcc                                                                                                                                    >  3:26658/1‑139 (MQ=255)
                  aaTGCCGCAGAACACAATGACCGTTTGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATc                                                                                                                        >  8:60565/1‑139 (MQ=255)
                      ccGCAGAACACAATGACCGTTTGGACCAAGGCCTGCTGAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACTATTTAGAACAGAAT‑CATCAAGACAAGCTGCGATAGCGAAACCCCGGCCGCTACCCCGGTCGCTa                                                                                                                    <  3:305344/139‑1 (MQ=255)
                         cAGAACACAATGACCGTTTGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAAt                                                                                                                  >  8:305870/1‑138 (MQ=255)
                         cAGAACACAATGACCGTTTGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAAt                                                                                                                  >  3:85890/1‑138 (MQ=255)
                         cAGAACACAATGACCGTTTGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATg                                                                                                                 <  7:60565/139‑1 (MQ=255)
                         cAGAACACAATGACCGTTTGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATg                                                                                                                 <  1:142967/139‑1 (MQ=255)
                            aaCACAATGACCGTTTGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAAc                                                                                                              <  2:229374/139‑1 (MQ=255)
                                 aaTGACCGTTTGGACCAAGGCCTGCTCAGAAAATTCAAGTTTCGCTGCCGATTTG‑CATGCGCCAATTTT‑AACAGGATACTTCAAGCAAACATACGATTGCGAAACCCGGGCCGGTCCCCCGAGCGGTAAGAAACGGaa                                                                                                          >  4:139353/1‑138 (MQ=255)
                                 aaTGACCGTTTGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGaaa                                                                                                         >  4:77598/1‑139 (MQ=255)
                                   tGACCGTTTGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATa                                                                                                       <  7:305870/139‑1 (MQ=255)
                                   tGACCGTTTGGACCAAGGCCTGCAAATAAAAATGCAGTTTCGCAACCGCTTTGCCAT‑CGACAATTTTTAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATa                                                                                                       <  7:113172/139‑1 (MQ=255)
                                       cggttGGCCCAATGGCCGCCCACAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGc                                                                                                   <  3:139353/136‑1 (MQ=255)
                                         tttGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCAt                                                                                                 <  7:162405/139‑1 (MQ=255)
                                         tttGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCAt                                                                                                 >  4:156314/1‑139 (MQ=255)
                                         tttGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCAt                                                                                                 >  8:162405/1‑139 (MQ=255)
                                           tGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATg                                                                                                >  7:269870/1‑138 (MQ=255)
                                             gACCAAGTCTTGATCATAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CCAAAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGAc                                                                                              <  8:269870/138‑1 (MQ=255)
                                                    gCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTg                                                                                      <  6:127439/139‑1 (MQ=255)
                                                    gCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CCACAATTTTGAACAGAAT‑CATCAAGACAAGCTGCCATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTg                                                                                      <  6:132816/139‑1 (MQ=255)
                                                       tGCTCAGAAAAATGGAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCAc                                                                                   >  7:281356/1‑139 (MQ=255)
                                                        gCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGCACCGAAATACAGCATGACATTTTCTGCACt                                                                                  >  1:288832/1‑139 (MQ=255)
                                                        gCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACt                                                                                  >  3:54455/1‑139 (MQ=255)
                                                              aaaaaTGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGAt                                                                            >  4:231085/1‑139 (MQ=255)
                                                                        tttCGCATCCGCTTTGAAAT‑CGACATTTTTGAACATAAT‑AATCAATACAAGCTTAGATATCTAAAAACCTGCCGATACACCGATCGATAATGAACCGAAATACATCATGACATTTTCTGCACTTAAGATGCGaatattt                                                                  <  8:281356/139‑6 (MQ=255)
                                                                          tCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAAttt                                                                 >  2:208275/1‑138 (MQ=255)
                                                                             cacccGGTTTCACAC‑CGACAATTTTGAACACAAA‑CAAACACACAAGCATCGATATCGAAACCCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATg                                                             <  4:40242/136‑1 (MQ=255)
                                                                                  gCTTTCACAT‑CCCAAATTTTGAACATAAT‑CAACACCACAAGCTTCGATATCGAAACCCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCg                                                        <  6:198078/139‑1 (MQ=255)
                                                                                                            gAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACAGccc                               <  3:231085/139‑1 (MQ=255)
                                                                                                             aaT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACAGccc                               >  3:45293/1‑138 (MQ=255)
                                                                                                              at‑catcAAGACACGCGTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACAGCCCGa                             <  3:213564/139‑1 (MQ=255)
                                                                                                              at‑catcAAGACAAGCTTCTATATCCAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACAGCCCGa                             <  4:85890/139‑1 (MQ=255)
                                                                                                              at‑catcAAGACAAGCTTCGATATCGAAACCCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACAGCCCGa                             <  4:45293/139‑1 (MQ=255)
                                                                                                              at‑catcAACACAAGCTTCGATATCGAAAACCCGGCAGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACTGCCCGa                             <  3:51543/139‑1 (MQ=255)
                                                                                                                        aCAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACAGCCCGATTTCTTTAc                    >  5:237420/1‑139 (MQ=255)
                                                                                                                            gCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACAGCCCGATTTCTTTACTCCg                >  1:42799/1‑139 (MQ=255)
                                                                                                                                      cGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACAGCCCGATTTCTTTACTCCGTCTCTtaat       >  1:268542/1‑138 (MQ=255)
                                                                                                                                        aaaaCCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGAc                                                                                              <  7:111897/49‑1 (MQ=255)
                                                                                                                                        aaaaCCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGAc                                                                                              >  8:111897/1‑49 (MQ=255)
                                                                                                                                          aaCCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACAGCCCGATTTCTTTACTCCGTCTCTTAATaaaaa  <  4:54455/139‑1 (MQ=255)
                                                                                                                                          aaCCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACAGCCCGATTTCTTTACTCCGTCTCTTAATaaaaa  <  4:26658/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
GATCATGATCAAAAGGTAAATGCCGCAGAACACAATGACCGTTTGGACCAAGGCCTGCTCAGAAAAATGCAGTTTCGCATCCGCTTTGACAT‑CGACAATTTTGAACAGAAT‑CATCAAGACAAGCTTCGATATCGAAAACCCGGCCGCTACCCCGATCGCTAATGAACCGAAATACAGCATGACATTTTCTGCACTTAAGATGCGAAAAATTTTATGCTTCGTCATTCCAATTAATTGAAACAGCCCGATTTCTTTACTCCGTCTCTTAATAAAAA  >  CP006881/2928686‑2928960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑